Q热贝氏柯克斯体间接ELISA检测方法的建立

Abstract

Abstract: In this study, the whole bacteria protein of the Coxiella burnetii attenuated strain phase Ⅱ was used as the antigen and then the indirect ELISA to detect Q fever Coxiella burnetii was established. Through optimizing the concentration of the coated phase Ⅱ antigen, the concentration of the second antibody and the reaction condition, we confirmed that the critical value of this method was 0.44. 393 clinical bovine sera were detected by this method and IDEXX Q fever (Coxiella burnetii) Antibody Test Kit. The results showed that the sera positive detection rates were 11.45% and 6.10%, respectively. Compared with the results of the two detection methods, the coincidence was 94.66%. The results demonstrated that sensitivity and specificity of the established indirect ELISA detection method were better and it could be used for the clinical diagnosis of the antibody of Q fever Coxiella burnetii.

Key words: Q fever; Coxiella burnetii; indirect ELISA; phase Ⅱ antigen

WU Wen-jun,JIA Guang-le,LIAO Juan-hong2,MEI Lin,HAN Xue-qing,LIN Xiang-mei,ZHANG Ying. Establishment of Indirect ELISA Method to Detect Q Fever Coxiella burnetii[J]. .

References

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Establishment of Indirect ELISA Method to Detect Q Fever Coxiella burnetii

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