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Bioinformatics Analysis of MSTN Gene in Sansui Duck

YI Heng-jie1,2,3,LI Hui1,2,3,YANG Sheng-lin1,2,3,ZHAO Zhong-hai1,2,3,PENG Bang-xing1,2,3   

  1. (1.College of Animal Science, Guizhou University, Guiyang 550025,China;2.Key Laboratory of Animal Genetics, Breeding and Reproduction,Guizhou Province, Guiyang 550025,China;3.Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025,China)
  • Received:2013-10-18 Online:2014-04-20 Published:2014-05-27

Abstract: In this study, the coding region of MSTN gene was amplified by PCR and sequenced by direct sequencing. Some characters of the MSTN gene encoding protein, including the physico-chemical properties, singal peptide, ransmembrane structure, secondary structure, high structure and functional domains, were analyzed by bioinformatics tools. The results showed that MSTN protein of Sansui duck was a kind of labile protein with high hydrophobicity, which was probably being singnal peptide. The protein contained 22 α-helics,26 β-sheets,26 T-turns,20 irregular coils and 1 transmembrane domain, and it had the main feature of TGF-β superfamily. The result provided an important reference for its further research.

Key words: Sansui duck; MSTN gene; bioinformatics