三穗麻鸭SOCS1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2018.03.004

Abstract

Abstract:

In order to analyze the molecular characteristics of suppressors of cytokine signaling (SOCS) gene in Sansui duck,and predict the biological function of SOCS1 gene encoded protein,the SOCS1 gene was amplified,cloned and sequenced,the secondary structure,conserved domain analysis,transmembrane domain,signal peptide and tertiary structure of SOCS1 protein were predicted using bioinformatic methods.The results showed that the length of SOCS1 gene was 624 bp,encoding 207 amino acids.The SOCS1 gene of Sansui duck was shared a nucleotide identity of 97.6%,92.6%,68.3%,65.0%,64.8%,64.5%,63.5% and 58.2%,and an amino acid identity of 99.5%,96.6%,69.2%,64.0%,64.0%,67.9%,65.4% and 50.8% with those of Anser cygnoides,Gallus gallus,Xenopus laevis,Sus scrofa,Bos taurus,Homo sapiens,Rattus norvegicus and Ctenopharyngodon idella,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between Sansui duck and Anser cygnoides.The secondary structure of the encoding protein was based with random coil,alpha helix,beta turn and extended strand region,with a central SH2 domain and a SOCS box.There was no the transmembrane domains and the signal peptide,and with the curved spiral tertiary structure.These results provided a theoretical basis for the study of the biological function of SOCS1 gene encoding protein in Sansui duck.

Key words: Sansui duck; SOCS1 gene; encoding protein; molecular characterization

CLC Number:

Q785

LONG Lishu, HUA Min, WAN Run, OU Deyuan, GOU Wanli, WEN Ming. Cloning and Sequence Analysis of SOCS1 Gene in Sansui Duck[J]. , 2018, 45(3): 590-597.

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Cloning and Sequence Analysis of SOCS1 Gene in Sansui Duck

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