三穗麻鸭Cofilin2基因序列分析及其组织表达研究

doi:10.16431/j.cnki.1671-7236.2018.04.003

Abstract

Abstract:

In order to analyze the characteristics of Cofilin2 gene and its expression in tissues of Sansui ducks,the specific primers were designed according to the sequence of Gullus Cofilin2 gene in GenBank,and amplified for Cofilin2 gene in Sansui ducks,then the Cofilin2 gene characteristic was analyzed using bioinformatic softwares,and the mRNA level of Cofilin2 gene in different tissues of Sansui ducks were detected with Real-time quantitative PCR.The results showed that the length of Cofilin2 gene was 498 bp in Sansui ducks,which encoded 166 amino acids.The identities of Cofilin2 gene in Sansui ducks were 94.4%,91.6%,91.6%,87.6%,77.7%,70.5% and 69.5% shared with that of Gallus,Macaca mulatta,Bos taurus,Mus musculus, Salmo salar,Homo sapiens and Sus scrofa,respectively.The phylogenetic tree analysis result showed that Cofilin2 gene maintained a highly conservative among different species.The secondary sructure of this encoding protein was consistes of alpha helix,extended strand,beta turn and random corn,and the curved spiral in tertiary structure.Real-time quantitative PCR detection results showed that there were different degrees expression of Cofilin2 gene in heart,liver,spleen,lung,kidney,brain,thymus,duodenum,leg muscle and bursa of Fabricius,the expression of Cofilin2 gene was the highest in heart,and that was the lowest in bursa of Fabricius.The results provided some basic data for the molecular mechanism of Sansui duck diseases.

Key words: Sansui ducks; Cofilin2 gene; cloning; Real-time quantitative PCR

CLC Number:

Q786

HUA Min, HE Xinwei, WAN Run, CHENG Zhentao, ZHOU Bijun, WEN Ming. Sequence Analysis of Cofilin2 Gene and Its Expression in Different Tissues of Sansui Ducks[J]. , 2018, 45(4): 858-865.

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Sequence Analysis of Cofilin2 Gene and Its Expression in Different Tissues of Sansui Ducks

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