胰岛素样生长因子-Ⅰ、肝细胞生长因子、转化生长因子-β1、干扰素-γ对奶牛乳腺上皮细胞增殖活性的影响

Abstract

Abstract: This experiment was conducted to investigate the regulation of insulin-like growth factor-Ⅰ（IGF-Ⅰ）, hepatocyte growth factor （HGF）, transforming growth factor-β1 （TGF-β1） and interferon-γ （IFN-γ） in the growth of bovine mammary epithelial cells in vitro.The bovine mammary epithelial cells were isolated by explant culture technique and differential adhesion method and identified by immunochemistry.The effect of cytokines regulating the growth of bovine mammary epithelial cells in different concentrations was measured by MTT.The results showed that when the concentrations of IGF-Ⅰ and HGF were 10 to 200 and 0.1 to 100 ng/mL, respectively, the growth of bovine mammary epithelial cells increased. While when the concentrations of TGF-β1 and IFN-γ were 2.5 to 100 and 5 to 160 ng/mL, respectively, the growth of bovine mammary epithelial cells decreased in a dose-dependent manner.These results indicated that IGF-Ⅰ and HGF could upregulate the proliferation of bovine mammary epithelial cells in vitro in a dose-dependent manner.TGF-β1 and IFN-γ could inhibit the proliferation of bovine mammary epithelial cells in vitro.

Key words: cytokine; mammary epithelial cells; primary culture; cell proliferation

LI Ying-ying, XIA Xiao-jing, WU Yun-di, HE Bo-ping, YAN Xin-wu, GAO Yuan-yuan, WANG Yu-ping, LEI Lian-cheng. Effects of IGF-Ⅰ, HGF, TGF-β1, IFN-γ on Proliferation of Bovine Mammary Epithelial Cells[J]. .

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Effects of IGF-Ⅰ, HGF, TGF-β1, IFN-γ on Proliferation of Bovine Mammary Epithelial Cells

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