马/驴源马链球菌马亚种新疆分离株小鼠感染模型的建立及验证

doi:10.16431/j.cnki.1671-7236.2025.03.035

Abstract

Abstract: 【Objective】 This study was aimed to explore the conditions and methods of the infection mice model induced by Streptococcus equi subsp. equi from horse/donkey,and lay a foundation for the development of new drug of horse/donkey strangle and related research.【Method】 100 Kunming mice were selected and infected with different concentrations of hourse source Streptococcus equi Xinjiang strain (SEE-M) and donkey source Streptococcus equi Xinjiang strain (LXY019),the minimum complete lethal dose (MLD) was determined.Then 30 Kunming mice were randomly divided into 3 groups (Model Ⅰ:SEE-M strain infection;Model Ⅱ:LXY019 strain infection;Control:Normal saline),10 mice in each group.Mice were infected with the corresponding strain of 80% MLD to establish the model of strangle infection,and the death of mice was observed.By detecting the organ coefficient,visceral bacterial load,serum inflammatory factors,and histopathology of mice,the construction of the mouse infection model was verified.【Result】 The results showed that the MLD of mice infected with horse/donkey derived Streptococcus equi subsp.equi was 1.875×10⁹ and 8.750×10⁸ CFU,respectively.The horse/donkey strangle model was established by infecting mice with 80% MLD horse/donkey Streptococcus equi subsp. equi.The mortality rates were 60% and 70% respectively,and the body weight showed a downward trend.The experimental results of mice infection model showed that compared with control group,the liver,lung and kidney organ indexes of mice in model Ⅰ and model Ⅱ groups were significantly increased (P＜0.05).All organs of the mice in model Ⅰ and model Ⅱ groups showed Streptococcus equi subsp.equi infection positive,and the bacterial load in liver,spleen,lung and kidney was significantly higher than that in heart and brain (P＜0.05).The levels of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) in serum of mice in model Ⅰ and model Ⅱ groups were extremely significantly increased (P＜0.01).Pathological section results showed that the organs of mice in model Ⅰ and model Ⅱ groups showed obvious pathological changes such as inflammatory cell infiltration,bleeding,structural destruction,and necrosis and shedding of visceral cells.【Conclusion】 Infection of mice with 1.5×10⁹ CFU of Streptococcus equi subsp.equi from horses and 7×10⁸ CFU of Streptococcus equi subsp.equi from donkeys could successfully establish equine strangle animal model. All the organs of infected mice were infected by pathogenic bacteria,especially lungs,livers,spleens and kidneys.The results laid a foundation for the prevention and treatment of equine strangle and the development of targeted drugs.

Key words: Streptococcus equi subsp.equi; equine strangle; animal model; cytokine

CLC Number:

S852.61

LIU Dandan, LI Jinrong, GAO Hanya, LI Shuyi, PENG Fei, XU Liyina, WANG Xin, HAO Baoshan, MAI Zhanhai, WUSIMAN Adelijiang, ABULA Saifuding, LIU Jianhua, ZHANG Wei. Establishment and Verification of Mice Infection Model of Horse/Donkey Source Streptococcus equi subsp.equi in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1342-1351.

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Establishment and Verification of Mice Infection Model of Horse/Donkey Source Streptococcus equi subsp.equi in Xinjiang

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