藏猪促红细胞生成素的表达与低氧适应分析

Abstract

Abstract: In order to explore the relationship between the expression of erythropoietin (EPO) in kidney tissue of Tibetan pig and hypoxia adaptation, the Western blotting technique was used to detect EPO protein and the kidney tissue which was from Tibetan pig and Large Yorkshire in different altitudes was used in this study. The high altitude Tibetan pigs and Large Yorkshire pigs were from Linzhi of Tibet, and the low altitude were from Beijing and Hefei of Anhui. The results showed that the EPO protein of ZZ，ZB，YZ，YH in A,B and C three groups of experiments was in different expression. Through comparative and analysis, the EPO protein expression was significant difference between high altitude Large Yorkshire pigs and the high altitude Tibetan pigs (P<0.05), and it was extremely significant difference between high altitude Large Yorkshire pigs and the low altitude of Tibetan pigs and Large Yorkshire pigs in group A (P<0.01)；The EPO protein expression was no significant difference between high altitude Tibetan pigs and the low altitude Tibetan pigs (P>0.05), and it was significant difference between high altitude Tibetan pigs and the high and low altitude Large Yorkshire pigs in group B (P<0.05)；The EPO protein expression was significant difference between Tibetan pigs (P<0.05), and it was extremely significant difference between low altitude Tibetan pigs and the high and low altitude Large Yorkshire pigs in group C (P<0.01). When Large Yorkshire pigs were in the high altitude emergency state, the EPO protein expression of Large Yorkshire was higher than Tibetan pigs. When Yorkshire pigs had lived for a long time in the highlands, the EPO protein expression was lower than Tibetan pigs, but higher than the low altitude Yorkshire pigs. The stable expression of EPO was an essential factor of Tibetan pigs to adapt to high altitude hypoxia environment. And the high expression of EPO protein in high altitude Large Yorkshire’s kidney tissue was the physical adjustment process which could help Yorkshire pigs adapt to the plateau low oxygen environment.

Key words: Tibetan pig; Large Yorkshire pig; Western blotting; kidney tissue; EPO protein; difference; hypoxic adaptation

WU Hong,DEJI Bazhuo,ZHANG Jian,WANG Ya-fei,ZHANG Hao,CHAMBA Yangzom. EPO Protein Expression and Hypoxic Adaptation Analysis in Tibetan Pig[J]. .

References

1 刘金凤,商鹏,旦增旺久,等.藏猪促红细胞生成素（EPO）基因多态性分析［J］.湖北农业科学,2012,51(12):2590～2601.
2 许海霞.高海拔和低海拔部分家畜促红细胞生成素（EPO）基因遗传多态性研究［D］.兰州:甘肃农业大学,2012.
3 江炎庭,苟潇,李明丽，等. 高原民族和动物低氧适应血液生理特征及相关基因研究进展［J］.中国畜牧兽医, 2012, 39(10): 149～153.
4 汪家政,范明.蛋白质技术手册［M］.北京:科学出版社,2000.
5 郑庆棠.红细胞生成素受体研究新进展［J］.汕头大学医学院学报,1994,1:132～134.
6 段爱华.促红细胞生成素的研究进展［J］.中国全科医学,2008,11(2A):264～267.
7 Baker J E. Erythropoietin mimics ischemic preconditioning［J］.Vascul Pharmacol, 2005, 42（5～6）:233～241.
8 Chen C, Sytkowski A J. Erythropoietin activates two distinct signaling pathways required for the initiation and the elongation of c-myc［J］. J Biol Chem, 2001, 276（42）：38518～38526.
9 Guyton A C. Textbook of medical physiology［J］.The American Journal of the Medical Science,1967,242（2）:136.
10 Hall J E, Guyton A C. Textbook of medical physiology［M］.New York: Saunders, 2011.
11 Jacobson L O,Goldwasser E, Feied W, et al. The role of the kidney in erythropoiesis［J］.Nature,1957,179(2):633～634.
12 Jelkmann W. Molecular biology of erythropoietin［J］.Intern Med，2004,43(8):649～659.
13 Kaplan S M, Rothmann S A, Gordon A S, et al. Extrarenal sites of erythrogenin production［J］.Proc Soc Exp Biol Med,1973,143(2):310～313.
14 Lai P H, Everett R, Wang F F, et al. Structural characterization of human erythropoietin［J］. J Biol Chem，1986, 261(7):3116～3121.
15 Maiese K, Li F, Chong Z Z.New avenues of exploration for erythropoietin［J］.JAMA, 2005,293(1):90～95.
16 Semenza G L, Nejfelt M K, Chi S M, et al. Hypoxia-inducible nuclear factors bind to an enhancer element located 3′ to the human erythropoietin gene［J］. Proc Natl Acad Sci USA,1991,88(13):5680～5684.
17 Siren A L, Fratelli M, Brines M, et al. Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress［J］. Proc Natl Acad Sci USA,2001,98(7):4044～4049.
18 Zanjani E D, Poster J, Burlington H, et al. Liver as the primary site of erythropoietin formation in the fetus［J］.J Lab Clin Med,1977,89(3):640～644.

[1]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[2]	DONG Wanyu, ZHANG Jumin, BIAN Sushu, ZHOU Yingshan, DU Jing, YU Haoran, ZHONG Wei, SONG Houhui, WANG Xiaodu. Comparative Analysis of Environmental Factors in Fattening House Under Two Ventilation Modes in Hangzhou Large-scale Pig Farm [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 421-428.
[3]	NIE Jingru, MA Li, YAN Dawei, DENG Jun, ZHANG Hao, ZHANG Bo, LIU Jinqiao, DONG Xinxing. Analysis of Differentially Expressed Genes and Regulation Pathways of Intramuscular Fat Deposition in Large Diqing Tibetan Pigs at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2855-2868.
[4]	XUE Jiajia, QIN Lihong, CAO Yang, GAO Yi, LIU Lixiang, LI Xu, XIAO Cheng, WANG Jing, ZHANG Guoliang. Cloning of Ephrin A5 Gene and Its Expressing in Different Tissues in Red Steppe [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1967-1975.
[5]	MA Li, NIE Jingru, LU Shaoxiong, LIU Shiyi, WANG Lin, LI Jintian, YAN Dawei, DONG Xinxing. Comparison of the Taste Activity Value of Full Spectrum Free Amino Acid in Muscle of Diqing Tibetan Pig and Wild Boar×Diqing Tibetan Pig [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1275-1283.
[6]	LI Qianwen, YANG Zhen, YANG Yanan, WANG Zhengwen, WEN Yuliang, CAI Yuan, ZHAO Shengguo. Effects of Environmental Temperature in Winter and Summer on Physiological Characteristics and Related Gene Expression of Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 873-881.
[7]	ZHAO Xialing, XIAO Qi, QIAN Wenxian, WEN Dongxu, YAN Mingshuai, LYU Lixin, NIU Jiaqiang, HE Kongwang. Isolation,Identification and Biological Characteristics of Staphylococcus chromogenes from Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 3014-3021.
[8]	MENG Jun, WANG Jianwen, KONG Qisen, ZENG Yaqi, LI Linling, ZHANG Yaang, REN Wanlu, WANG Chuankun, YAO Runchen, YAO Xinkui. Effect of Training on Body Angle in 1 km Trotter of Yili Horse [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 814-821.
[9]	ZHAO Hang, SONG Shanshan, WANG Jianke, LIN Peng. Bioinformatics Analysis of Feline importin Gene and Its Dynamic Expression in F81 Cells Infected with Canine Parvovirus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3861-3869.
[10]	WANG Zhenmei, DUAN Mengqi, LU Fenghui, WANG Shixin, QIANGJIUZHUOMA, TAN Zhankun, ZHANG Jian, SHANG Peng. Polymorphism and Tissue Expression Patterns of CKMT2 Gene in Tibetan and Yorkshire Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3305-3313.
[11]	KONG Zimeng, JIANG Bo, CAI Yunhong, HE Houjun, LI Yongqing, JIN Huan. Cloning,Protein Expression and Purification of Chicken Complement Receptor 2 Gene and Preparation and Identification of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 201-208.
[12]	XIAO Cheng, JIN Haiguo, WEI Tian, GAO Yi, YU Yongsheng, ZHANG Lichun, MA Huihai, CAO Yang. Cloning and Expression Analysis of SPARCL1 Gene Coding Region in Small-tail Han Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3466-3474.
[13]	FAN Yiping, WANG Yanfang, TAO Cong. Expression and Methylation Analysis of UCP3 Gene in Adipose Tissue of Bama and Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3627-3634.
[14]	LIU Lixiang, GAO Yi, LYU Yang, XUE Jiajia, HU Zhongchang, ZHANG Guoliang. Cloning of Acot2 Gene and Its Expression Difference of Different Tissues in Red Steppe [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3154-3162.
[15]	AN Qingming, ZHOU Huitong, WANG Xing, WU Zhenyang, LUO Yuzhu, JON G. Hickford. Association Analysis of UCP1 Gene Variation with Growth and Carcass Traits in Ovine [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3289-3298.

EPO Protein Expression and Hypoxic Adaptation Analysis in Tibetan Pig

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments