Abstract: In order to clone sequence of the coding region of the goat c-Myc gene， this study designed specific primers by refering to c-Myc genes coding sequence reported in GenBank including sheep，cattle，pigs，horses，etc.，and then amplified c-Myc coding sequence from embryonic skin tissue of goats by using RT-PCR technique and had sequence analysis. The result indicated that CDS of goat c-Myc gene had 1320 bp length including the stop codon. For only 10 differences comparing to sheep c-Myc gene nucleotide sequence （GenBank accession number：Z68501.1)，homology reached 99.17% and homology of deduced amino acid sequence reached 99.09%. Analysis of homology with other species showed that homology of goat c-Myc gene coding region nucleotide sequence was 91.2%，92.0%，90.2%，86.9%，86.0% with pigs，wolves，humans，rats，mice，and homology of deduced amino acid sequence was 93.6%，96.1%，92.3%，91.1%，89.6%. Using bioinformatics software analysis showed that goat c-Myc protein was localized in the nucleus and contained a HLH domain. This study further researched the function of c-Myc gene and laid the foundation of investigating the effect in the process of goat somatic cell reprogramming.

Key words: c-Myc gene; gene cloning; sequence analysis