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Stydy on Expression Regulation and Regulatory Functions of miRNA-205 in Pig Oocyte in vitro Maturation

CHEN Chao,LI Wan-hong, SUN Li-na,CHEN Shu-xiong,CHEN Lu,LI Chun-jin,ZHOU Xu   

  1. College of Animal Science, Jilin University, Changchun 130062,China)
  • Received:2013-12-16 Online:2014-04-20 Published:2014-05-27

Abstract: The research aimed to assess the expression profile and regulatory functions of miRNA-205 in pig oocyte in vitro maturation. The potential target of miRNA-205 was predicted by biology software including TargetScan and microRNA.org. The expression profile of miRNA-205 and its target gene were detected by Real-time PCR in pig cumulus-oocyte complexes(COCs) in vitro maturation 0, 24 and 48 h. The expression of miRNA-205 in pig COCs maturation gradually increased, 0 h lowest, 48 h maximum. The expression of miRNA-205 was significantly reduced in mature solution added EFG, FSH and LH compared with which in a basic mature solution, the expression of PTX3 and cumulus proliferation-related genes including TNFAIP6 and HAS2 were significantly increased. The expression of PTX3 was negative correlation with that of miRNA-205 in porcine oocyte maturation. From these results could be concluded that the miRNA-205 conserved sequence specifically bound to 3′-UTR region of PTX3 gene might be participated in the regulation of oocyte maturation.

Key words: miRNA-205; cumulus-oocyte complexes; Real-time PCR; porcine oocyte maturation