小鼠乳腺发育泌乳过程中L型氨基酸转运载体1的表达研究

Abstract

Abstract: To investigate the underlying relationship between amino acid transporter expression and mammary gland development, the expression of LAT1 and 4F2hc in virgin, pregnancy, lactation and involution of mouse mammary gland was detected by RT-PCR and laser confocal microscope. The results showed that LAT1 and 4F2hc located on the membrane of ductal epithelial cell, myoepithelial cell and adipocyte in virgin mammary gland. The expression of LAT1 and 4F2hc was lower because duct development was slow in this period. In pregnancy, LAT1 and 4F2hc located on the basolateral of ductal epithelial cell. The expression of LAT1 and 4F2hc went up as ductal epithelial cell proliferation increased. In lactation, LAT1 and 4F2hc located on the basolateral of acinar epithelial cells. The expression of LAT1 and 4F2hc was the highest because of milk protein synthesis. In involution, acinar structure collapsed. The expression of LAT1 and 4F2hc decreased. All these results revealed that LAT1/4F2hc was an important amino acid transporter in mouse mammary gland. The expression of LAT1 and 4F2hc was related to amino acid requirement in mammary gland development and lactation.

Key words: mammary gland development; amino acid transportation; LAT1; 4F2hc; expression

LIN Ye, HU Hong-liu, HOU Xiao-ming. Study on Expression of L-type Amino Acid Transporter 1 in Development and Lactation of Mouse Mammary Gland[J]. .

References

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Study on Expression of L-type Amino Acid Transporter 1 in Development and Lactation of Mouse Mammary Gland

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