miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响

doi:10.16431/j.cnki.1671-7236.2015.07.009

Abstract

Abstract: MicroRNAs (miRNAs) are a group of small,non-coding RNA molecules about 22 nucleotides to regulate a wide variety of important biological processes,including cell proliferation,differentiation,apoptosis as well as the progression of tumors.Liposome transfection was used to detect the expression of miR-142-3p in human mammary epithelial cells,the effects of miR-142-3p on the cell proliferation,apoptosis and milk protein synthesis were detected by Real-time PCR,Western blotting,cell proliferation analysis.The results indicated that after miR-142-3p being silenced,prolactin receptor (PRLR) protein was increased,at the same time the expressions of related pathways protein AKT,mTOR,STAT5 and cyclinD1 were increased,the ability of cell proliferation was increased.The results suggested that in human mammary epithelial cells,the silence of miR-142-3p could increase the expression of PRLR protein,miR-142-3p could promote the synthesis of milk protein and increase the proliferation of mammary epithelial cells by regulating related pathways proteins AKT,mTOR,STAT5 and cyclinD1.

Key words: miR-142-3p; prolactin receptor (PRLR); mammary gland development; proliferation

CLC Number:

Q527

XIE Xue-jiao, LUAN Sheng, WANG Yuan, ZHANG Li, CUI Ying-jun, WANG Chun-mei, LI Qing-zhang. Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein[J]. , 2015, 42(7): 1680-1685.

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Effect of miR-142-3p on Cell Proliferation of Human Mammary Epithelial Cells MCF-10A and Synthesis of Milk Protein

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