L型氨基酸转运载体1对奶牛乳腺中β-酪蛋白表达的影响

doi:10.16431/j.cnki.1671-7236.2016.10.007

Abstract

Abstract:

In order to investigate the effect of LAT1 on β-casein expression in mammary epithelial cells of dairy cows,the LAT1 gene of dairy cow was amplified by PCR and pIRES2-EGFP-LAT1 expression vector was constructed.Then pIRES2-EGFP-LAT1 expression vector was transfected into mammary epithelial cells.The effects of LAT1 overexpression on expression of LAT1,4F2hc and β-casein were detected by Western blotting after 48 h of transfection.The results showed that pIRES2-EGFP-LAT1 was transfected into mammary epithelial cells successfully.The expression of LAT1 was extremely significantly increased (P<0.01) and β-casein was significantly increased (P<0.05) when LAT1 was overexpressed,while the expression of 4F2hc was not significantly changed (P>0.05).These results suggested that LAT1 had an important role in promoting milk protein synthesis in mammary epithelial cells of dairy cow.

Key words: dairy cow; mammary epithelial cell; LAT1; β-casein

CLC Number:

Q786

XING Wei-nan, DUAN Xiao-yu, YANG Yang, HOU Xiao-ming, LIN Ye. Effect of L Type Amino Acid Transporter 1 on β-casein Expression in Mammary Epithelial Cells of Dairy Cows[J]. , 2016, 43(10): 2553-2559.

References

[1] Hediger M A,Aromero M F,Peng J B,et al.The ABCs of solute carriers:Physiological,pathological and therapeutic implications of human membrane transport proteins introduction[J].Pflugers Arch,2004,447(5):465-468.
[2] Haining Z,Kawai N,Miyake K,et al.Relation of LAT1/4F2hc expression with pathological grade,proliferation and angiogenesis in human gliomas[J].BMC Clin Pathol,2012,12:4-11.
[3] Storey B T,Fugere C,Lesieur-Brooks A,et al.Adenoviral modulation of the tumor-associated system L amino acid transporter,LAT1,alters amino acid transport,cell growth and 4F2/CD98 expression with cell-type specific effects in cultured hepatic cells[J].Int J Cancer,2005,117(3):387-397.
[4] 史良会,张才全,彭小壮.LAT1、CD98在胃癌组织中表达与临床病理特征的关系[J].中国临床药理学与治疗学,2011,6(12):1361-1366.
[5] 董艳,武卫党,慈小燕,等.L-型氨基酸转运体1在肿瘤诊断和治疗中的研究进展[J].天津医药,2015,43(12):1456-1459.
[6] Hayashi K,Jutabha P,Endou H,et al.LAT1 is a critical transporter of essential amino acids for immune reactions in activated human T cells[J].J Immunol,2013,191(8):4080-4085.
[7] 冯丽丽,林叶,李庆章.奶牛不同发育时期乳腺组织LAT1和4F2hc的表达[J].中国乳品工业,2011,39(4):4-7.
[8] 陆东林,张丹凤,刘新丽,等.牛奶中的氨基酸含量及其营养价值[J].新疆畜牧业,2001,4:12-14.
[9] 王俊锋,黄静,梁国义.泌乳反刍动物乳蛋白合成机理及调控途径研究[J].中国乳业,2005,2(9):24-27.
[10] Nakamura E,Sato M,Yang H,et al.4F2(CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer[J].J Biol Chem,1999,274(5):3009-3016.
[11] Smith Q R.Transport of glutamate and other amino acids at the blood-brain barrier[J].J Nutr,2000,130(Sl):1016-1022.
[12] Napolitano L,Scalise M,Galluccio M,et al.LAT1 is the transport competent unit of the LAT1/CD98 heterodimeric amino acid transporter[J].Int J Biochem Cell Biol,2015,67:25-33.
[13] 林叶,胡红柳,侯晓明.小鼠乳腺发育泌乳过程中L型氨基酸转运载体1的表达研究[J].中国畜牧兽医,2014,41(4):143-147.
[14] Alemán G,López A,Ordaz G,et al,Changes in messenger RNA abundance of amino acid transporters in rat mammary gland during pregnancy,lactation,and weaning[J].Metabolism,2009,58(5):594-601.
[15] Roos S,Kanai Y,Prasad P D,et al.Regulation of placental amino acid transporter activity by mammalian target of rapamycin[J].Am J Physiol Cell Physiol,2009,296(1):142-150.
[16] 马婧,谭毅,谭冬梅,等.氨基酸转运载体LAT1对小鼠胎盘早期建立的影响[J].中国实验动物学报,2015,23(3):256-260.
[17] Chen Z,He P,Ding X,et al.PPARγ stimulates expression of L-type amino acid and taurine transporters in human placentas:The evidence of PPARγ regulating fetal growth[J].Sci Rep,2015,5:12650-12662.
[18] Rosario F J,Dimasuay K G,Kanai Y,et al.Regulation of amino acid transporter trafficking by mTORC1 in primary human trpphoblast cells[J].Clin Sci,2016,130(7):499-512.
[19] Poncet N,Mitchell F E,Ibrahim A F,et al.The catalytic subunit of the system L1 amino acid transporter (Slc7a5) facilitates nutrient signalling in mouse skeletal muscle[J].PLoS One,2014,9(2):e89547.
[20] Rosario F,Kanai Y,Powell T,et al.Mammalian target of rapamycin signalling modulates amino acid uptake by regulating transporter cell surface abundance in primary human trophoblast cells[J].J Physiol,2013,591(3):609-625.
[21] Mikereit R,Persaud A,Vanoaica L,et al.LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosome and promotes mTORC1 activation[J].Nat Commun,2015,6:7250.
[22] Fotiadis D,Kanai Y,Palacín M.The SLC3 and SLC7 families of amino acid transporters[J].Mol Aspects Med,2013,34(2-3):139-158.

Effect of L Type Amino Acid Transporter 1 on β-casein Expression in Mammary Epithelial Cells of Dairy Cows

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