鹅细胞色素P450 2C45（CYP2C45）基因多态性及其与产肝性能的关联分析

Abstract

Abstract: In order to study the influence of genetic variation in CYP2C45 gene on fatty liver production performance, PCR and sequencing methods were used to identify polymorphism of CYP2C45 gene exons in 113 Landes goose, and the association of polymorphism with production performance of goose fatty liver were investigated. The results indicated that there were 4 SNPs in exon 5 at 111 bp of C>T transition and at 147 bp of C>T transition, and in exon 7 at 71 bp of A>G transition and at 135 bp of G>A transition, respectively. These SNPs were in the stage of complete linkage by linkage disequilibrium analysis. Combining 4 SNPs as one genotype, there were AA (CCAG/CCAG) and AB (CCAG/TTGA) genotypes, but BB genotype (TTGA/TTGA) was not found， the genotype frequencies were 0.9027 and 0.0973, respectively, and the gene frequencies of alleles A and B were 0.9513 and 0.0487, respectively. Analysis of variance showed that the body weight of AB genotype was higher significantly than AA genotype (P<0.05), although the liver weight and ratio of liver weight versus body weight of AA genotype were higher than AB genotype, there were no significant difference （P>0.05）. It suggested that, in the case of the same yield of liver production, less body weight less feeding, AA genotype maybe contribute to the improvement of production efficiency.

Key words: Landes goose; CYP2C45 gene; SNP; fatty liver

ZHANG Rui, ZHANG Yi-hui, SUN Xiao-xian, GONG Dao-qing. Association Analysis of Polymorphism in the CYP2C45 Gene with Fatty Liver Production in Geese[J]. .

References

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Association Analysis of Polymorphism in the CYP2C45 Gene with Fatty Liver Production in Geese

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