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Table of Content

20 November 2010, Volume 37 Issue 11
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动物营养与饲料科学
Duodenal Infusion of Fatty Acids with 18C Affects Immunity of Dairy Cows
YANG Guang;;WANG Jia-qi;BU Deng-pan;KHAS-Erdene;LIU Qing-sheng;SUN Peng;ZHOU Ling-yun;LUO Qiu-jiang
2010, 37(11):  5-8. 
Abstract ( 1207 )  
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Evaluated effects of duodenal infusion of fatty acids with 18C (18-C FA; C18∶0，C18∶1，C18∶2 and C18∶3) on immunity of dairy cows. The total concentration of immunoglobulin (Ig) in serum，T lymphocyte subsets in peripheral blood，the acount of white blood cell (WBC) and body temperature were determined. Duodenal infusion of 18C FA did not affect Ig in serum and body temperature. Duodenal infusion of C18∶2 tended to increase the amount of CD4+T lymphocyte. Duodenal infusion of C18∶3 and C18∶2 decreased the acount of WBC significantly. Duodenal infusion of C18∶1 tended to increase the acount of monocyte. Duodenal infusion of C18∶3 and C18∶2 increase and regulate immunity of dairy cows.

Application of Pentosan Research to Feed Industry
LI Qing-feng;MA Xiang-dong
2010, 37(11):  9-12. 
Abstract ( 1288 )  
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As the main component of non-starch polysaccharides in feed stuff，pentosan is the most part of anti-nutritional components in livestock and poultry feed，but also the evaluation indicator of feed stuff performance and effect of enzyme preparations. In order to promote scientific use of non-conventional feed stuff and pentosanase，the latest research progress on pentosan can be used in feed industry was reviewed，physical and chemical properties，distribution，methods of content measurement and anti-nutritional effects were described in detail.
Effect of the Alfalfa Hay on the Production of Cows andEconomic Benefit
YAN Xu-dong;XU Yu-peng;WANG Jun-ping;YUE Ming-qiang;JI Jian-jun
2010, 37(11):  13-15. 
Abstract ( 1445 )  
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40 multiparous，lactating Holstein cows were assigned to 4 groups according to milk yield，body weight，parity and days in milk with a randomized design. No difference was existed among groups. the control group diet include straw and concentrated feed, add different levels of alfalfa hay to the test group, analysis of cows production performance and economic benefits of the impact. The results showed that the test group and control group differences in dry matter intake significantly (P＜0.05), experimental group significantly increased milk yield, the difference was significant (P＜0.01),fat percentage reduction, the difference was not significant (P＞0.05), milk protein, non-fat milk solid was significantly increased (P＜0.01). Somatic cell count decreased significantly (P＜0.05).Adding alfalfa to the diet,the quality of milk to be significantly improved. Cost-effectiveness analysis showed that diets supplemented with alfalfa dairy industry can significantly increase the overall efficiency of cultivation, breeding chains.
Progress of Application and Research of House-flies Larva
XU Li-lan;SHAN An-shan;CHEN Peng
2010, 37(11):  16-19. 
Abstract ( 1200 )  
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With the scale enlargement of livestock breeding and rapid development of feed industry，in recent years，house-flies larva and its extractive were investigated extensively，such as house-flies larva hydrolysis，grease，chitosan and antibacterial peptide. House-flies larva contains many kinds of fatty acid，mineral，vitamin，amino acid and metabolizable energy. In addition，some deep investigations such as the physical and chemical property，application，and medical value of the extracting peptide，grease and chitosan from house-flies larva have become new research areas in the 21st century. Research has shown that these extractives were useful to regulate the nutrition metabolism，improve production performance of livestock and poultry. The author reviewed the application and research advancement of house-flies larva in animal production，in order to provide theoretical and practical basis for conserving protein feed，develop new kinds of safe antibacterials and feed additives.
The Research Progress of Yeast Selenium in Swine’s Diet
KUANG Sheng-yao;TANG Ling;ZHANG Chun;ZHANG Jin-xiu
2010, 37(11):  20-21. 
Abstract ( 1250 )  
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Yeast selenium is good organic selenium in feed and it has better absorptivity and biological security compared with inorganic selenium. It can improve the growth, survival rate and reproduction ability of swine, and it can also improve the quality and nutrition of pork. The application, research and development of yeast selenium in swine’s diet are discussed in this paper.
生理生化
Molecular Structure and Function of Interferon Regulatory Factor-1
WANG Dai-wei;DENG Xue-mei
2010, 37(11):  22-24. 
Abstract ( 1309 )  
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Interferon regulatory factor 1(IRF-1) is a important multifunctional transcription factor for regulating of expression of interferon(IFN) and having cantivirus action，antineoplastic activity. Indeed，IRF-1 selectively modulates different sets of genes，depending on the cell type in order to evoke appropriate responses in each. The author summarizes the structure，the transcriptional regulation and the multiple biological effects.
Research Progress and Perspective in Skin Follicle of Cashmere Goat
LV Xue-feng;ZHENG Wen-xin
2010, 37(11):  25-28. 
Abstract ( 1232 )  
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The author reviewed research progress and perspective of skin follicle in cashmere goat from five aspects that include structure, development regularity, culture in vitro, hair follicle stem cell and genes related hair follicle development. Finally, the development of goat in future was prospected.
Effects of Conjugated Linoleic Acid on Lysozyme Levels of Macrophage in Broilers Immunosuppressed with Cyclophosphamide
CHEN Yan-hong;LIU Dan;ZHOU Lei;LI Li-min;YAN Zhong
2010, 37(11):  29-31. 
Abstract ( 1211 )  
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The experiment was carried out to study the effects of conjugated linoleic acid (CLA) on lysozyme secretion of 〖HT〗〖ST〗〖WT〗


〖WT5”BZ〗microphages in broilers immunosuppressed with cyclophosphamide (CY).Twenty-four 1-day-old male Arbor Acre broiler chickens were randomly allocated into 2 treatments，with or without CY injection. CY was injected into the femoral muscle of broilers at a dose of 80 mg/kg of body weight for three consecutive days (days 14，15 and 16).On day 21，the peritoneal exudate macrophages from 12 broilers per treatment were collected for in vitro culture. The different levels (0，25，50 and 100 μmol/L) of two CLA isomers (c9，t11-CLA and t10，c12-CLA) and their mixture (50/50) were used for the macrophage in vitro culture. The results showed that t10，c12-CLA alleviated the suppressed effects of CY on the secretion of lysozyme of macrophages，and the higher t10，c12-CLA supplementation had better effects.But c9，t11-CLA had no significant influence on lysozyme contents of macrophages.

The Oxidative Damage of High Protein Diet on Pancreas Function in Mice
GU Chun-mei;;SHI Yong-hui;LE Guo-wei
2010, 37(11):  32-35. 
Abstract ( 1110 )  
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To estimate the oxidative damage of high protein diet on of pancreas function in mice, thirty male C57BL/6J mice were randomly divided into three groups: control group, high protein group and cysteamine group. Each group was fed for 2 weeks, and then pancreas samples were collected to examine pancreas function. The results showed that ingestion of high protein diet markedly increased contents of MDA and decreased T-AOC and activities of SOD and GSH-Px (P＜0.05). Pancreas weight and concentration of protein, DNA and RNA were significantly increased. Digestive enzyme activities were significantly decreased and levels of somatostatin and insulin were enhanced (P＜0.05). Supplemented cysteamine decreased free radical levels and increased antioxidative capability, and improved pancreas function. These data demonstrated that high protein diet can increase oxidative damage of free radical on pancreas function through destroying balance of oxidant and antioxidant.
Effects of Genhuang Dispersible Tablets on Part of the Physiological and Biochemical Function of Broilers
XIN Rui-hua;LUO Yong-jiang;ZHENG Ji-fang;WANG Gui-bo;LUO Chao-ying;XIE Jia-sheng;HU Zhen-ying;LI Jin-yu;LI Wei
2010, 37(11):  36-38. 
Abstract ( 1189 )  
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The purpose of this experiment is to study the effect on part of the broiler physiological and biochemical function of Genhuang dispersible tablets. 120 19-day-old sanhuang broilers were randomly divided into four groups including the low dose group, medium dose group, high dose group and the control group, and every group had thirty broilers. The drug was continuously administrated for 30 days. At the 10th, 20th, 30th days after administration, we randomly selected 10 broilers to examine the weight, organ index, liver and kidney function indicators. The results showed that the boilers’ weight of each group had no significant differences compared with the control group (P＞0.05). There was no regularity between the organ index and drug’s dosages while the drug had little effect on the serum alanine amino transferase activity and creatinine levels. The results of the research showed that Genhuang dispersible tablets had small interference on weight, kidney and liver functions, and organ index of the broilers. So it was a low toxic and safe traditional Chinese medicine preparation which was suitable for clinical use.
生物技术
Tandem Expression and Identification of EG95s Gene of Echinococcusgranulosus and C3d Gene Using Baculovirus Expression System
YU Lin-lin;;JIA Hong;HOU Shao-hua;LIU Dan;DING Min;GUO Xiao-yu;ZHU Hong-fei
2010, 37(11):  44-49. 
Abstract ( 1172 )  
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This study was conducted to prepare for comparison of the prokaryotic and eukaryotic EG95-(C3d)3 proteins in the immune effects and for development of efficient method of detection Echinococcosis by building recombinant baculovirus containing three copies of C3d gene and EG95s gene of Echinococcus granulosus. EG95s gene of and three tandem copies of goat C3d 〖JP2〗gene were inserted to pTarget vector to obtain recombinant plasmid, named pTarget-EG95-(C3d)3, then the target gene, EG95-(C3d)3,were cloned into the pFastBac-Hta of Bac-to-Bac system by BamH I and Xba I, the recombinant plasmid,pFastBacHta-EG95-(C3d)3, were obtained and then〖JP〗 was transformed into the DH10Bac for transposon reorganization. The recombinant transposon, rBacmid-EG95-(C3d) 3, were transfected into Sf9 insect cells for obtain the EG95-(C3d)3 recombinant baculovirus and expression〖JP2〗 of EG95-(C3d)3 recombinant proteins. The proteins were identified by SDS-PAGE and Western blotting.rUsing the recombinant proteins detected sheep antibodies against Echinococcosis granulosa. The results showed that the EG95-(C3d)3 recombinant baculovirus was obtained, and the EG95-(C3d)3 recombinant protein with the size of about 132 ku were correctly expressed in insect cells in Sf9, the results of Western blotting showed that this EG95-(C3d)3 protein could response to Echinococcus granulosus-specific positive serum, indicating that EG95-(C3d)3 protein has immune activity. The recombinant proteins had well sensitivity with Echinococcosis granulosa. The EG95-(C3d)3 gene was correctly expressed in insect cells,which built the bases for comparison of the immune effects of prokaryotic and eukaryotic EG95-(C3d)3 proteins, and for development of efficient method of detection Echinococcosis.

Molecular Cloning and Sequnence Analysis of Honey bee Sacbrood Virus RNA-dependent RNA Polymerase Gene
ZHOU Liang;WANG Zhen-guo;SONG Zhan-yun;LIU Jin-hua;XUE Li-gang;WANG Quan-kai
2010, 37(11):  50-52. 
Abstract ( 1137 )  
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Honey bee sacbrood virus (SBV) is a kind of picornavirus, a small positive strand RNA virus, which is greatly threatened to the Chinese honeybee breeding. This experiment through the gene cloning of sacbrood virus disease and sequence analysis, found the high identity between the sacbrood virus sequence of Guangdong province, but it had difference in individual base, suspected that it may be a new type sacbrood virus.
Optimization of Electroporation for Transfection of Eimeria tenella
ZHAO Shi-yun;SU Hua-li;LIU Xian-yong;SUO Xun
2010, 37(11):  53-56. 
Abstract ( 1149 )  
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Low transfection efficiency has been the major hurdle for gene transfection in Eimeria tenella. In order to overcome this difficulty, the electroporation parameters were optimized for traditional electroporation apparatus of Bio-Rad Gene Pulser and newly developed Amaxa’s Nucleofector I device, respectively. By adjusting series of key parameters, which include the concentration of plasmid DNA, the electric field intensity, the ambient temperature and bult-in programs of Nucleofector I, we found the optimal condition for electroporation of E. tenella sporozoites is at a voltage of 2000 V and a capacitance of 25 μF, using 50 μg plasmids/107 sporozoites and cuvettes with a 4 mm gap, which together resulting in a transfection efficiency of 3.8×10-4.
Development of a Real-time PCR Approach for Quantification of Three Cellulolytic Bacteria in Rumen
YIN Chuan-bao;CHEN Jun;ZHU Qi;CHENG Zhen-tao;ZHOU Bi-jun;;WEN Ming;
2010, 37(11):  57-60. 
Abstract ( 1198 )  
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Study on the impact of intestinal microbial ecology of host animals by mucosal immunity with attenuated invasived bacteria，based on the 16S rRNA gene sequences of three cellulolytic bacteria including Ruminococcus albus,Ruminococcus flavefaciens and Fibrobacter succinogene,the primers were designed.Ruminal microbial total DNA was isolated from rumen content，and amplified by PCR.The PCR products was linked with pMD-18T Vector to construct recombinant plasmid and transfected into Escherichia coli.Target plasmids were selected by blue-white selection and their specificity was identified by direct PCR and DNA sequencing.The serial gradient concentrations of plasmid DNA were used to Real-time PCR.Results showed that neucleotide homology of 16S rRNA of three cellulolytic bacteria were higher than 99%.There were obvious differences in amplification curves of Real time PCR by the gradient concentrations of the recombinant plasmid，the correlation coefficient of the three standard curves were close to 1.The melting curves of Real-time PCR displayed a single peak.It was indicated that the standard plasmids and curves were constructed successfully and can be applied to the future research of mucosal immunity with attenuated Salmonella bacteria.
Cloning and Sequence Analysis of the 5′Flaning Region of Pituitary Transcription Specific Factor 1 Gene in Goose
ZHAO Rong-xue;XU Qi;ZHAO Wen-ming;QIAO Na;ZHANG Hai-bo;DUAN Xiu-jun;DONG Biao;CHEN Guo-hong
2010, 37(11):  61-65. 
Abstract ( 1188 )  
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In order to explore the mechanism of Taihu goose pituitary specific transcription factor 1 (Pit1) gene transcription regulation by studying the promoter region of goose Pit1 gene，the 5′upstream sequence of Taihu goose Pit1 gene was attained by Genome Walking PCR, sequencing and sequences alignment analysis. A 3.0 kb upstream sequence of Pit1 gene was cloned firstly by Genome Walking from goose genomic template and sequenced. The sequence analysis showed that the cloned region contained two promoters， and had typical TATA box and CAAT box， but GC box was not found. Many potential transcription factors binding sits were identified by TESS analysis， which might be regulate transcription and expression the Pit1 gene potentially. The alignment results showed that the conservative region spanned from transcription start site to -200 bp of upstream，the sequence was strongly conservative and it was potential core promoter. Meanwhile，goose and chicken had higher conservative. The results showed that the cloned sequence may be considered as the promoter region of Taihu goose Pit1 gene.
Cloning and Sequence Analysis of MDR1 Gene in Ivermectin-sensitive Collies
YU Yan-ying;LIN Yuan-qing;WU Xuan-guang;SUN Yong-xue
2010, 37(11):  66-68. 
Abstract ( 1197 )  
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The aim of this study was to investigate the feasibility and clinical significance of applying RT-PCR to the detection of the MDR1 mutation and establish a simple, economic, efficient method of genetic diagnosis. Genomic RNA was extracted from the blood of and ivermectin-nonsensitive collies. The MDR1 gene was amplified by RT-PCR, and then cloned and sequenced. The MDR1 mutation of Scotland Collies concerns an exonic 4 bp detection in the palindromic sequence GATAG and creates a nonsense frame shift at amino acid position 75 followed by a premature stop codon. RT-PCR is a simple, economic, quick, precise method of genetic diagnosis and was able to find MDR1 mutation in Scotland Collies in this study. It is helpful in clinical carries on the pharmacological treatment reasonably.
Progress on Hemagglutinin Gene Research of Swine Influenza Virus
ZHAO Meng-meng;PAN Jun-bin;WANG Heng
2010, 37(11):  69-72. 
Abstract ( 1246 )  
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Swine influenza, which is an acute, hermotropic, highly infectious viral respiratory disease of pigs, is caused by swine influenza virus. It can produce different kinds of bacterial or viral disease which is secondary and concurrency, so it is becoming one of the most hazardous dieases in the livestock husbandry gradually. The hemagglutinin gene, main antigen of swine influenza virus on the surface, is of crucial importance to the pathogenic of SIV, and the hemagglutinin protein also has rich biological effects. The research of swine influenza virus hemagglutinin gene will provide help to prevent swine influenza and offer ideas to develop new typies of vaccines.
Cloning and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 3 of Spirometra erinaceieuropaei Isolates from Hunan Province
WU Hui-lan
2010, 37(11):  73-75. 
Abstract ( 1377 )  
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The objectives of the present study was to examine sequence variation in the mitochondrial cytochrome c oxidase subunit 3 (cox3) gene among Spirometra erinaceieuropaei isolates from Hunan province. The partial cox3 (pcox3) was amplified from each S. erinaceieuropaei sample by PCR, and pcox3 sequences were aligned using the ClustalX 1.81. MP tree was constructed using the software Phylip 3.67 version 4.0. Sequence homology analysis was performed using the Megalign program of the software DNAStar 5.0 by comparing with the corresponding sequence of S. erinaceieuropaei available in GenBank. The results showed that the lengths of pcox3 were 264 bp. The phylogenetic tree showed that the Hunan isolates and the S. erinaceieuropaei available in GenBank were clustered in the same clade. The pcox3 sequences of S. erinaceieuropaei isolates from Hunan province were slight different. The results provided a foundation for further studies of molecular identification and molecular genetics of S.erinaceieuropaei.
Application of Enzyme-linked Immunosorbent Assay in Detection of Veterinary Drug Residues in Product of Animal
YU Hua;YAN Yu-bao;XIE jing;HU Juan;LIAO Dang-jin;ZHOU Min-jiang;CUI Peng-bo;YE Jian-qiang
2010, 37(11):  76-79. 
Abstract ( 1140 )  
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The negative effects of veterinary drug residues on food safety have become more and more obvious, so high requirements have been made for the rapid detection of veterinary drugs residues in food. This article introduces the basic principle of enzyme-linked immunosorbent assay (ELISA), classification and the key technologies for the establishment of ELISA. Aspects involved the application, problem and prospect of ELISA in the detection of veterinary drug residues in foods were reviewed in the paper.
Development and Application of Multiplex PCR in Differentiating B.abortus and B.melitensis
DU Tao-feng;HAN Wen-yu;LEI Lian-cheng;SUN Chang-jiang;LI Yang;GU Jing-min;XU Hui-hu
2010, 37(11):  80-83. 
Abstract ( 1196 )  
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Three pairs of primers were designed according to the specific gene BCSP31 of Brucella genus and insert sequence IS711. The DNA of B.abortus 544A, B.abortus104M and B.melitensis 16M strains were used as the positive control to establish the multiplex PCR assay. Developed a multiplex PCR procedure to identify two major species of the genus Brucella simultaneously. Bands of 301, 114 bp could be amplified from strains of B.abortus, and 301，253 bp from B.melitensis. The multiplex PCR assay could detect as low as 100 pg of mixed B.abortus 544A and B.melitensis 16M DNA. No specific bands could be amplified from control bacteria such as E.coli O157：H7 and Yersinia enterocolitica. 106 cow feces samples were determined by the developed multiplex PCR assay, and total 9 samples displied the B.abortus positive. Rose bengal plate agglutination test was used as controlled trial, and 9 samples were Brucella positive. The results indicated that the established multiplex PCR assay was highly sensitive and specific.This assay provided a molecular detection tool to differential diagnosis for brucellosis.
Progress on Genomic Selection in Animal Breeding
LI Ya-lan;;QU Hao;;SU Guo-sheng;SHU Ding-ming;
2010, 37(11):  84-89. 
Abstract ( 1217 )  
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Genomic selection （GS） is a form of marker-assisted selection in which genetic markers covering the whole genome are used so that all quantitative trait loci (QTL) are in linkage disequilibrium with at least one marker. Most research found that genomic selection had the advantages of shortening the generation interval，improving the selection efficiency, and reducing the cost remarkably, so it has been the research hotpot in the animal breeding field. This review focuses on the approaches to estimate the effects of the chromosome segments and their difference, the factors to influence the accuracy of GS, the advantages of genomic selection in animal breeding, the researches on the GS in the world. At last it points out that the challenge and the prospect of application GS in the animal breeding in China.
Proteomic Analysis of Individual Variation in Plasma of Dairy Cows Using Two-dimensional Gel Electrophoresis
YANG Yong-xin;CHENG Guang-long;ZHAO Hui-ling;JIANG Xi-chun;CHEN Sheng
2010, 37(11):  90-93. 
Abstract ( 1187 )  
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To investigate the interindividual variation of plasma proteome of dairy cows and evaluate the minimal size of samples for proteomic analysis. In this study, plasma samples in early lactation dairy cows were collected and the proteome profiles of these samples were analyzed using two-dimensional gel electrophoresis. After visualization proteins with Coomassie Blue G-250 solution, the coefficient of variation values of 56 protein spots were analyzed and ranged from 8.7% to 59.6%. The five proteins with coefficient of variation values ranged from 35% to 59.6% were identified by ion trap mass spectrometer, and three spots identified as transthyretin, apolipoprotein A1 and alpha 1 acid glycoprotein. Moreover, the one-way ANOVA feature showed a correlation between sample size and individual variations. The results showed that when the sample size exceeded 4, the individual variations were not significant to the whole pool. The results indicated that individual variations are encountered in plasma protein expression profiles and further for number of samples (sample size) needed to detect given protein expression differences with statistical significance.
遗传繁育
Study on the Diversity of Yunnan Pig in Meat characteristics Using NIR Spectroscopy Based on Principal Component Analysis and Cluster Analysis
WU Jin-liang;GAO Xin;YIN Yun-fei;CHEN Ji-hong;YANG Guo-ming
2010, 37(11):  94-99. 
Abstract ( 1252 )  
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The aim of the present study was to investigate the feasibility of identifying the relationship between the Yunnan pig breeds in meat characteristics with near-infrared (NIR) spectroscopy. One hundred and two pork samples were from eight countries of Yunnan province in China. Based on the NIR spectra of the preprocessing pork，the samples were subjected to principal component analysis (PCA) and cluster analysis (CA). The results showed that there were the common features and some differences in NIR spectra from different Yunnan pig breeds，seven pig breeds were scattered different regions of the principal components. According to the euclidean distance of NIR spectra, the relationship of the different breeds could be identified by cluster analysis，the distance of spectra was the shortest between the samples from Dahe wu pig and Dahe pig，Diqing zang pig was special significantly in meat characteristics. So applying near-infrared fingerprint spectroscopy can accurately and quickly analysis meat characteristics of local swine breeds，and find out the special resource of swine breeds in meat.
Primary Study on Identify of the Differential Proteins between Bovine Frozen and Fresh Spermatozoa Using 2-DE and MS
WU Cheng-jiang;;ZHU Hua-bin;WANG Dong;HAO Hai-sheng;DU Wei-hua;ZHAO Xue-ming;LU Yong-qiang
2010, 37(11):  100-104. 
Abstract ( 1171 )  
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Heat Trizol method was used to prepare the global protein of bovine spermatozoa, and the global proteins of fresh sperm and frozen sperm was separated by the technique of 2-DE with linear IPG (pH 3 to pH 7, 24 cm). 839±34 protein spots were obtained from the sample of frozen spermatozoa, while 564±16 from the sample of fresh spermatozoa. 19 differential proteins between frozen and fresh sperm were detected after analysis and significance test with software of ImageMasterTM 2D Platinum, 9 of which were expressed up-regulated while one of which was down-regulated in the sample of frozen spermatozoa. In addition, 9 spots were found only in frozen spermatozoa. After enzymolysis and MS detection, mass spectrum information of 3 differential proteins were obtained, and the differential proteins were considered to be neutral sphingomyelinase(N- SMase) activation associated factor(FAN), glutathione-S-transferase(Mu5) and zinc ion binding structure of bovine heart cytochrome c oxidase in the fully oxidized state, which were related to cell stress and apoptosis. It was presumed that the increasing of these proteins significantly in frozen spermatozoa were involved with cryodamage of spermatozoa.
SNP of IGF-Ⅰ Gene and its Association with Carcass Traits in Chicken
JIN Wei;ZHANG De-xiang;LU Sheng-xia;QI Xiao-hong;WANG Dai-wei;YANG Liu;DENG Xue-mei
2010, 37(11):  105-109. 
Abstract ( 1156 )  
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In this study, the genetic polymorphism of insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of 5′region in 438 yellow feather dwarf broilers was detected by PCR-RFLP method. PCR products treated by Hinf Ⅰ appeared GG，GT and TT genotypes. χ2 test showed the population was in Hardy-Weinberg equilibrium in this locus(P>0.05). Variance analysis indicated gender had extremely significant effect (P<0.01) on 9 traits (body weight and carcass weight，cutaneous fat thickness，half-eviscerated weight，eviscerated weight，breast muscle weight，drumstick weight，abdominal fate weight) and significant effect (P<0.05) on intramuscular fat，that family affected 8 traits extremely significantly (P<0.01)，while that genotype effect was suggestively associated with abdominal fat rate (P<0.05)，approximately suggestively associated with abdominal fat weight (P=0.0669). Multiple comparison of different genotypes’ least square mean displayed abdominal fat weight and abdominal fat rate of genotype TT were significantly lower than that of genotype GT (P<0.05)，while that of genotype GG was no different significantly from genotype GT and TT (P>0.05)
Progress in Proteomics Assessment of Seminal Plasma Proteins with Emphasis on the Spermatozoa Function
LI Hong-yan;ZHAO Xing-xu;ZHANG Yong;YANG Yong-xin;XU Tie-shan
2010, 37(11):  110-114. 
Abstract ( 1170 )  
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Seminal plasma proteins show striking effects on spermatozoa function in a variety of reproductive processes, including acrosome reaction, sperm capacitation, sperm storage, competition, and fertilization. Seminal plasma proteins were isolated and identified for a long history，several of major seminal plasma proteins have been identified，such as bovine seminal protein, heat shock protein, cystein-rich secretory protein. Proteome is applied successfully in seminal plasma proteins research, has given to new research strategies and improved the efficiency of researchers in exploring the unknown field of seminal plasma. This review introduced some essential information about seminal plasma proteins associated with spermatozoa function and progress in seminal plasma proteome, and provides valuable information to clarify the relationship between seminal plasma proteins and sperm capacitation, sperm storage, and fertilization function at the protein level.
Research Progress on the Roles of H-FABP Gene in the Fatty Deposition
YOU Xiao-yan;YANG Fei-yun;XIE Yue-wei
2010, 37(11):  115-118. 
Abstract ( 1075 )  
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eart fatty acid binding protein (H-FABP) is one member of fatty acid binding protein family, which has important effect in binding long-chain fatty acids and oxidation, so we researched it as one of the candidate genes affecting fatty deposition. This paper reviewed the relationship between the H-FABP gene and fatty deposition in pig, chicken and cattle, investigated the practical meaning as candidate gene. 
Study on Sex Control of Bovine Embryos in vitro Fertilization〖
LI Xiao-chen;;LI Hua;;CAI Qin;CAO Hui;HUANG Ying;
2010, 37(11):  119-122. 
Abstract ( 1140 )  
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In vitro fertilization (IVF) technique has been maturity and more perfect, so it had setting solid foundation for the development and industrialization in cow in vitro -propagation. In this study, pregnancy rate and calf sex ratio of the non-sex control IVF embryos with artificial insemination were compared, and it was found that pregnancy rate was same between two groups and the proportion of male in the IVF group was higher than internal fertilized group. Selecting the sex control and non-sex control seminal fluid to carry out IVF test, followed by molecular identification of cow SRY gene for the non-sex control and the sex control IVF embryos. The results showed that the male proportion of sex control IVF embryos was obviously lower than the non-sex control group. In addition, the authors also compared the blastocyst rate of the sex control with non-sex control IVF embryos, but there was no-remarkable difference between the two groups. It demonstrated that sex control IVF could be used to accelerate the industrialization of high-quality cow embryos. This study encouraged that sex control IVF embryos propagation will be the applied prospect of improving dairy industry, and realize the sex control embryos industrialization.
Reseach Progression about Cryoprotectant in the Process of Mammalian Sperm Cryopreservation
PING Shu-huang;YANG Shi-hua
2010, 37(11):  123-127. 
Abstract ( 1136 )  
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Sperm cryopreservation can provide a simple，economical，effective way to preserve biologic genetic resources, and it has become an important means and applied in many domains such as the treatment of human infertility，livestock breeding and biodiversity conservation. Adding cryoprotectant can be effective avoiding or decreasing sperm cryodamage and chilling in the appropriate stage of sperm cryopreservation. The review contained information relating to the effect of cryoprotectant on mammalian sperm vitality in the process of sperm cryopreservation，and the topics discussed include the types of cryoprotectant，cryoprotectant selection and methods of its addition and removal.
Progress in Reprogramming Somatic Cells into Induced Pluripotent Stem Cells
WANG Kai-yun;CHEN Li-wen;LIU De-wu;WU Zhen-fang
2010, 37(11):  128-131. 
Abstract ( 1481 )  
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Induced pluripotent stem(iPS) cells have the ability to self-renew while maintaining pluripotency. The generation of iPS cells would allow creation of patient-specific stem cells and disease-specific stem cells, they could be very useful to study various disease mechanisms and provide valuable tools for drug discovery. This article provides a review of recent progress in iPS cell research，including optimization methods to establish iPS cells，reprogrammed cell types，differentiation potential of iPS cells，elite and stochastic models for iPS cells，and applications of iPS cells.
Polymorphism Analysis of Exon5 of 2,4-dienoyl-CoA Reductase1 Gene in Sheep
LIU Dong;LIU Gui-lin
2010, 37(11):  132-135. 
Abstract ( 1178 )  
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According to exon 5 sequences published by GenBank of 2, 4-dienoyl-CoA reductase 1(DECR1) gene of bovine（NP_001068891）, one pair primer was designed to amplifiy exon 5 of DECR1 gene by using PCR technique for three sheep populations including of German MuttanMerino sheep, Dorper sheep and Texel sheep, the single nucleotide polymorphism of three sheep populations were detected by PCR-SSCP, and compared and analyzed with other species of chicken, rat, sheep, pig, human, bovine, rabbit, dog from GenBank. The results showed that the size of DECR1 gene exon 5 was 137 bp, SNPs was not found in exon 5 of DECR1 gene by PCR-SSCP. By means of sequence homology aligment between sequence of exon 5 of sheep DECR1 gene and others species, the results showed that homology of different species DECR1 gene was 81.02% to -97.08%, the highest similarity was 97.08％ between sheep and bovine. The molecular phylogenetic tree constructed by DECR1 gene exon 5 sequences showed that sheep clustered with bovine firstly，and secondly with rabbit and dog, and then human and pig. This results indicated that sheep had the most nearest relative relationship with bovine, but the far-thest with chicken. It was consistent with the orthodox taxonomic results, and DECR1 gene was very conservative in the progress of animal evolution
Research Progress of Regulation in Stem Cell Differentiation
HU Min-hua;GUO Xin-zheng;ZHANG Shou-quan
2010, 37(11):  136-139. 
Abstract ( 1134 )  
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Stem cells can sustain self-renewal and generate all cell types of the body in vitro, the special function of the stem cells makes it become the hot spot in the area of regenerating medicine, tissue engineer and traumatic repair. Understanding the regulation mechanism of differentiation in stem cells is the precondition and the theoretical basis of stem cell application. Here we summarize the research progress of stem cell differential regulation, in order to provide the reference of stem cells research.
Recent Advance of OB-R
ZHANG Shan-peng;WANG Jun;LOU Yu-jie
2010, 37(11):  140-142. 
Abstract ( 1094 )  
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Leptin encoded by obese gene（ob gene), is a protein secreted mainly by fat cells, which is mainly responsable for regulating energy metabolism and reproductive activity. Like other hormones, Leptin must combine to its receptor before it performs its specific physiological functions. In order to provide reference for further investigating the role of Leptin, structure, tissue distribution, biological functions and signal transduction of OB-R were reviewed in this paper.
疾病防治
Establishment of Indirect Haemagglutination Assays for Detection of Antibodies against Actinobacillus pleuropneumoniae Serotype 3
ZHAO Yan-zong;HE Dong-sheng;SU Dan-ping
2010, 37(11):  143-145. 
Abstract ( 1114 )  
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The indirect hemagglutination assays was obtained by sensitization of sheep red blood cell with the separated and purifitied capsular polysaccharide (CPS) of Actinobacillus pleuropneumoniae serotype 3. The result showed that the speciality of the indirect hemagglutination assays was good. It could only agglutinate the same type positive antibody and could be used to detect Actinobacillus pleuropneumoniae serotype 3 antibody. Simple fast economic way to test Actinobacillus pleuropneumoniae antibody with the indirect hemagglutination assays. It is a valid means to diagnose and prevent Actinobacillus pleuropneumoniae.
Resistance to Five Anticoccidial Drugs of Eimeria tenella Isolated from Changzhou Area
GAO Yan-feng;MAO Xue;TAO Jian-ping;XU Jin-jun
2010, 37(11):  146-149. 
Abstract ( 1222 )  
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One Eimeria tenella isolated from Changzhou district of Jiangsu province was studied in a battery test for evaluating drug resistance against five commonly used anticoccidial drugs including sulfamethoxydiazine and diaveridine permix, sulfachloropyrazine sodium, toltrazuril, maduramycin and decoquinate. Suqin yellow chickens were randomly divided into seven groups of 10 each, which contained five medicated groups, one uninfected and unmedicated control, and one unmedicated and infected control. Chickens of medicated groups and infected control were prophylactic medicated and infected with sporulated oocysts at the age of 14 days. Drug resistance was assessed for each drug by calculating the relative oocyst production (ROP), reduction of lesion scores (RLS), percentage optimum anticoccidial activity (POAA) and anticoccidial index (ACI) . Results revealed that the E. tenella isolated from Changzhou district were sensitive to decoquinate, complete resistant to other four drugs. It suggested that in Changzhou district E. tenella was seriously resistant to drugs, decoquinate can be uesd, and the other drugs should be used reasonably or not be used at present.
Reason, Detection and Prevention for Dairy Cows with Subclinical Ketosis
JIA Zong-fei;HAN Yi-chao;SHI Jian-peng;PANG Quan-hai;ZHAO Kui
2010, 37(11):  150-152. 
Abstract ( 1210 )  
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Subclinical ketosis has no obvious symptoms, and it has brought great impact on dairy cattle industry. This article reviewed from the harm, causes, detection, prevention and control of subclinical Ketosis, and provided a scientific basis for further study on diagnosis and treatment of cows with subclinical ketosis.
Analysis of Resistance for 92 Diarrheic Escherichia coli Isolates from Pig Farms
ZHANG Jun-feng;;CHEN Lin;WEI Dong-xia;HUANG Dong-zhang;FU Wen-gui;ZHAI Shao-qin;ZHOU Shu-lan
2010, 37(11):  153-155. 
Abstract ( 1104 )  
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The resistance Escherichia coli isolates from 92 diarrheic pig rectal swab samples were analyzed to 17 antibacterial agents for the rational using principle. Total of 92 diarrheic pig rectal swab samples were collected to isolate and identify Escherichia coli.Then all isolates were tested for susceptibility to 17 antimicrobial agents by the broth microdilution method. 92 Escherichia coli isolates recovered from diarrheic pigs were identified. And their resistant ratio were orderd as sulfamethoxazole（100%）,trimethoprim（100%）, kanamycin（89%）,gentamicin（80%）, chloramphenicol（78%）, streptomycin（76%）,neomycin（76%）, tetracycline（73%）, spectinomycin（72%）, enrofloxacin（66%）, ampicillin（50%）, amikacin（45%）, florfenicol（32%）, apramycin（27%）, rifampicin（23%）, cefazolin（22%）, ceftiofur（0%）. All isolates were multiple resistant isolates and 81 types of resistant spectrum were presented in 92 Escherichia coli isolates. These Escherichia coli isolates were resistant against 14 antibacterial agents at most, and 3 antibacterial agents at least. Therefore wide resistant spectrum and high resistant ratio were presented in the 92 diarrheic pig Escherichia coli isolates.
Investigation on Infection of Duck Circovirus in Part Regions of Guangxi Province
QU Su-jie;SU Yan-qiong;ZHENG Min;QIN Yong;MO Sheng-lan;LU Wen-jun;LIANG Yuan;YAN Si-gang
2010, 37(11):  156-158. 
Abstract ( 1214 )  
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To investigate the epidemic situation of duck cirovirus (DuCV) in Guangxi, 321 duck tissue samples collected from Baise, Liuzhou and Guilin cities of Guangxi province were detected by PCR. Results showed that the specific fragments of 228 bp were amplified from 58 samples, with a total positive rate of 18.07%. Furthermore, positive samples could be found in all 3 cities. And positive rate of each cities were 18% (54/300), 5.6% (1/18) and 100% (3/3), respectively. This investigation demonstrated that the existence of DuCV infection with different levels in duck populations from vary regions of Guangxi province.
The Effect of Compound Chinese Traditional Medicine Powder on Meat-miscellaneous Chicken in Immunity
YIN Wei-wei;WANG Hong-mei;WU Jia-ping;SUN Ji-guo
2010, 37(11):  161-163. 
Abstract ( 1097 )  
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300 meat-miscellaneous chicken of 1 day are divided into 3 groups randomly. They are comparison, test 1, test 2. It is 5 repetitions of 20 chickens each. The meat-miscellaneous chickens are immunised by routine. The feed with compound Chinese traditional medicine powder has effects on the weight and immunity of meat-miscellaneous chicken. The results indicate that the difference of antibody of NDV in test 1 and test 2 is obvious in 14, 21 d (P<0.05), not obvious in 3 groups in 28, 35 d (P>0.05). The powder has good effects on meat-miscellaneous chicken in NDV; test 1 and test 2 is obvious in IBDV antibody in 21 d (P<0.05), not obvious in 28, 35 d. The immune indexes indicate that the test group is higher than the contrast (P<0.05). It shows that it strengthens body fluid immunity with compound Chinese traditional medicine powder.
Antibacterial Experiment Research of Chelidonine with Different Extraction Methods
DONG Xiao-qing;GE Chen-xia;WANG Yan-zhuo;JIANG Jing
2010, 37(11):  166-167. 
Abstract ( 1246 )  
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Choosing the six common pathogenic bacteria to obtain the six kinds of extraction MIC value by detecting. The result showed that antibacterial material of Chelidonine mainly focus on the parts of polarity small, polarity large water n-buoh without bacteriostatic effect.
Research of Support System for Animal Epidemics Emergency Management Based on GIS
LI Lin;HE Jian-bin;DONG Jing;YANG Yong
2010, 37(11):  168-171. 
Abstract ( 1107 )  
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It is necessary to build a support system for animal epidemics emergency management to dynamic monitor and control the outbreak and spread of disease such as HPAI and FMD. Strong functions of data management analysis and display in the GIS can be combined with animal epidemics to build a support system, which can be used to support epidemics emergency management to decrease loss.
流行病防治
Serological Detection and Analysis of Toxoplasma gondii Infection in Dogs in Lanzhou
ZHENG Hong-xing;YE De-he;QI Shan-shan
2010, 37(11):  172-173. 
Abstract ( 1195 )  
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In order to find the infection information of Toxoplasma gondii infection in dogs in Lanzhou and the different detection results in different test methods. 272 dogs from veterinary surgeons were detected to find the masccline of Toxoplasma and the detection rates were statistical analysed; 66 pieces of blood sample were detected by indirect hemagglutination test and enzyme-linked immunosorbent test, the two methods were compared. The results showed that there was a relationship between the infection of Toxoplasma and the age of dogs, the infection rats increased corresponding to the increasing of age, the infection rate was 3.77% in less than one years old dogs, 10.59% in one to three years dogs, 15.28% in three to five years dogs, 22.58% in more than five years dogs. There were no significant deviation in different gender（P>0.05）; there was no significant deviation in detection results of HA and ELISA through t test comparison（P＞0.05）. The method of IHA was applied to epidemiological study in large scale.
兽医临床
The Pathological Analysis of Canine Bladder Transitional Cell Carcinoma
WANG Long-tao;GE Chen-xia;XIANG Xiu-li;HUANG Lei;WANG Li-li;HU Gui-xue
2010, 37(11):  174-175. 
Abstract ( 1101 )  
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The physical diagnosis which contains the general examination and B-Scan were carried out for the feeble 10-year-old female golden retriever. Because of the expectant treatment is ineffectual so we decided to carry out the operation. Then the lump was exsected and fixed by 10％ formalin. The paraffin section was made to observe the change of this abnormal tissue. Eventually, the final diagnosis showed that this old dog suffered from canine bladder transitional cell carcinoma. This thesis discussed the etiological factor, the pathologic mechanism, the diagnostic methods and treatment method of this disease.
Pathology Observation of a Wild Goose Infected by Newcastle Disease
LIU Wen;;JIN Zhao;LI Yong;;ZHANG Ning;;MA Li-min;;LI Lin;;XIA Zhi-ping
2010, 37(11):  176-179. 
Abstract ( 1109 )  
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In this experiment, we had made observations on the general pathology and histopathology changes of a wild goose, which was infected by Newcastle disease. Obvious hemorrhage and necrosis were found in the intestinal tract, glandular stomach and endocardium, in accordance to the characteristics of Newcastle disease infection. Necrotic changes were found in small intestine, spleen and liver, renal glomerulus was also serious atrophied through histopathology observation. The results have offered references for the study of pathohistology of wild grew waterfowl infected by Newcastle disease.
Measurement of Resistance Index of Renal Arteries in Healthy Dogs
ZHUANG Rui-hong;ZENG Jian-ying;ZHAN Yao-ming
2010, 37(11):  180-181. 
Abstract ( 1126 )  
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The purpose of this research was to examine healthy dogs by using color doppler ultrasonography, establish healthy dogs’ doppler ultrasound indices. Nineteen healthy dogs were tested by B-mode ultrasonic, CDFI and PW. The results were analyzed to establish the doppler ultrasound index of healthy dogs (showed as mean ± standard deviation). This study found resistance index of renal arteries was positively repeatable. The data collected form left and right kidneys were similar, which could be considered as normal ultrasound index.
Research on Behavior Test in Quarantine Detector Dog
CHENG Lu-guang;LI Zheng;PENG Jian-guo;LI Fei-xiang;LI Jie;LONG Ling
2010, 37(11):  182-184. 
Abstract ( 1108 )  
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Behavior test become more and more important in the research on quadrant detector dog’s training and working. Behavior test classifies dogs by comparing and stating stimulated dog’s behavior or non-stimulated dog’s, which is tested in a special state. It is used to assess the behavior of puppies, dogs-in-training and working dog, including two methods-qualitative analysis and quantitative analysis. Individual information may affect the veracity of behavior test. This review gives a brief summary of behavior test’s definition, use and standard.
The Treatment of Electro-acupuncture Combined with Drugs on Canine Intervertebral Disc Herniation
WANG Gui-bo;LIU Peng;DING Ming-xing;LUO Chao-ying;LI Jin-yu;WANG Dong-sheng
2010, 37(11):  185-187. 
Abstract ( 1093 )  
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In recent years, canine intervertebral disc herniation has been more and more common in pet clinic. As the pathogenesis of this disease is complex and the disease process is chronic, it’s very difficult to diagnosis. Meanwhile the cure rate will be low if surgical treatment of the disease is applied. Clinical practice showed that the application of electro-acupuncture combined with drugs for treatment of the disease would improve the cure rate substantially. In this paper, the causes and diagnosis of canine intervertebral disc herniation were described, and the mechanism of acupuncture combined with drugs for the treatment of the disease was explained. So it would provide theoretical guidance for the disease diagnosis and lay a solid foundation for the electro-acupuncture combined with drugs treatment programs to promote.
Diagnosis and Pathological Analysis of Coccidiosis in Chickens
WANG Long-tao;GE Chen-xia;HUANG Lei;MA Lei;WANG Dan;HU Gui-xue
2010, 37(11):  188-189. 
Abstract ( 1111 )  
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Pathological anatomy diagnosis was taken for the chickens natural infection coccidia cases, chickens blind mucosal scraping material was smeared and found a large number of coccidia oocysts by microscopic observation. Dead chickens cecal tissue was collected, fixed with 10% neutral formalin, embedded paraffin, HE staining, histological analysis. The results showed that the parasitic worms was main in the intestinal villus epithelial cells, a large number of intestinal villi were destructed, fractured and fell off, and cellulose- necrotic enteritis lesions was serious.
动物生产
Study on the Grazing Cattle Feeding with Non-protein Nitrogen Supplementation
GUAN Chao;YANG Xiao-min;ZHANG Xi-zhong;LI Jun;ZHANG Yuan-qing
2010, 37(11):  203-204. 
Abstract ( 1110 )  
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The experiment was conducted to investigate the effect of non-protein nitrogen supplementation on the grazing cattle. Thirty Chinese Simmental bull Taihang (weighted 150 to 170 kg) were chosen and divided into 3 groups, which were control group, experiment group 1 and experiment group 2. One kilogram corn flour was supplemented in experiment group 1 and 0.25 kg NPN supplementation was added at the basis of experiment group 1 in experiment group 2. The experiment was conducted at the time before grazing, which is the late spring and early summer, and lased 100 days. Results showed that the average daily gain was 0.622,0.839 and 1.051 kg for the control group, experiment group 1 and experiment group 2, respectively. Average daily gain was increased 217 g for the experiment group 1 than the control group and increased 429 and 212 g for experiment group 2 than control group and experiment group 1, respectively. The difference among three groups was remarkable significant, and weight gain differed significantly between experiment group 2 and control group. It was concluded that small amount of concentrate supplementation has obvious effect on the grazing cattle in northern slopes and small amount of NPN supplementation would have more significant economic benefits.
Effects of Spraying Modes on the Performance of Layer Ducks Reared in Intensive House without Pool
IANG Xiao-bing;LIN Ru-long;WANG Ji-mao;WANG Jun-hui;CHEN Hong-ping
2010, 37(11):  205-208. 
Abstract ( 1087 )  
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A total of 720 one-day old laying ducks were randomly divided into four groups to determine the laying performance, fertility and hatchability．The control group was reared in traditional mode with pool．Experimental group one was reared in intensive mode without pool but with intermittent spray by pipeline 4 times a day half an hour once．Experimental group two and group three were reared in intensive mode without pool but with intermittent spray by showerheads 4 times a day and 3 times a day,half an hour once．The results showed that the experimental group two got significantly higher laying performance and better feed conversion ratio (P<0．05)than experimental group three with same showerhead, but different spraying frequency. The total egg number of 500 day-old experimental group one was 329.4, feed conversion ratio was 2.85,which were no significant difference(P>0.05) with experimental group two with same spraying frequency, but significantly higher (P<0.05)than the control group and experimental group three. The total egg weitht of 500 day-old experimental group one was 22.513 kg,which was no significant different (P＞0.05),higher than the control group,but extremly significantly higher (P＜0.01) than the experimental group three,The spraying water consumption of experimental group one was less than experimental group two. There were no significant difference(P>0.05)in fertility and hatchability between the four groups. Thus the mode of experimental group one was selected to be the best breeding mode for the layer ducks reared in intensive house without pool but with intermittent spray by pipeline four times a day on the yards the best spraying mode .
经验交流
Development and Application of Enriched Cages of Layers〖
CHEN Dong-hua;LI Jian-hong;BAO Jun
2010, 37(11):  209-212. 
Abstract ( 1328 )  
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In order to improve the welfare conditions of laying hens in cages,enriched cages were produced and applied widely in the developed countries.This article introduced the current status of the development of the enriched cages in many countries,and the effects of the enriched cages on the layers were discussed.At last,the application of the enriched cages in China in the future was evaluated.
The Comparative Analysis of Three Local Chickens on Carcass and Body Size Traits
HAN Hai-xia;;LI Fu-wei;;CAO Ding-guo;;LEI Qiu-xia;;ZHOU Yan;;LI Gui-ming;;LIU Tao;LU Yan;;HUANG Bao-hua;
2010, 37(11):  213-215. 
Abstract ( 1017 )  
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Chickens including 30 Wenshang luhua chickens,30 Jining bairi chickens and 30 Laiwu black chickens were used for comparative analysis on carcass performance at the age of 170 d. The results showed that live body weight of the Jining bairi chickens was highest, reaching 1532.03 g, Laiwu black chickens was 1431.20 g and Wenshang luhua chicken was 1369.83 g (P<0.01).the Laiwu black chickens had the highest carcass percentage, percentage of half-eviscerated weight, percentage of eviscerated weight, and percentage of quarter, and significantly (P<0.05) or very significantly (P<0.01).From 15 min to 24 h post slaughter, the pH values of muscles displayed a declining trend, and these values were within the pH range of the normal muscles. The content of intramuscular fat of the Jining bairi chickens was the highest, the shear force of leg muscle and the content of intramuscular fat was the lowest in Jining bairi chickens. all varieties of body feature close to,the Wenshang luhua chickens had the smallest hip bone width and shank circumference, the biggest was Laiwu black chickens.
A Review: Analysis of Bovine IgG in Milk and Milk Products
ZHAO Sheng-guo;WANG Jia-qi;ZHANG Yu-tao;HU Xiao-li;BU Deng-pan;ZHOU Ling-yun
2010, 37(11):  216-218. 
Abstract ( 1105 )  
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The immunoprotective properties of bovine milk IgG have led to a recent proliferation of nutritional products incorporating this protein. These kinds of products are always priced by the content of IgG. It has therefore become critical that reliable analytical techniques for the measurement of the IgG content in such products are available. This literature summarized the universal method in the world including separation-based chromatography, electrophoresis, immunoassay and physical techniques. Meanwhile we compared the advantage and disadvantage of these kinds of methods.
Research in Chronic Toxicity Testing of Greater Celandine Alkaloids on Albinorat
QU Gui-juan;DONG Xiao-qing;WANG Yan-zhuo;CHENG Pei-ying
2010, 37(11):  222-223. 
Abstract ( 1026 )  
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For study on Chronic toxicity testing of greater celandine alkaloids to albinorat.Through inject different dose greater celandine alkaloids to albinorat,after 30 days,observe the indicator changes of albinorat’s behavior, blood physiology, biochemistry and viscera pathology.The experiment results showed that greater celandine alkaloids have no harmful effect to albinorat’s behavior, blood physiology, biochemistry and viscera pathology.
Determination of Residual Dicyclanil in Animal Food by HPLC
WU Hai-xia;FENG Pei-sheng;ZHANG Su-xia;LI Lin-xia;CHENG Lin-li;CUI Yao-wen
2010, 37(11):  224-226. 
Abstract ( 1050 )  
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A high-performance liquid chromatography method was developed for determination of dicyclanil in lamb and beef. The target compound was extracted from samples by acetonitrile, then was defatted by liquid-liquid extraction (LLE) with hexane, following cleaned up by a HLB SPE column. Finally, the prepared sample was determined by HPLC/UV at 270 nm. The calibration curve showed excellent linearity in the range of 20 to 1000 μg/L with correlation coefficient of 0.99. The mean average recoveries of dicyclanil ranged from 81.8% to 93.4% at spiked levels of 100 to 1000 μg/kg, with intra-assay (n=5) and inter-assay (n=3) coefficient of variation (CV) below 11.9% and 9.4%, respectively. The limit of detection (LOD) was 30.0 μg/kg for lamb and 28.0 μg/kg for beef. The limit of quantitation (LOQ) was 100.0 μg/kg for lamb and 93.1 μg/kg for beef. The results demonstrated that the method was simple, sensitive and accurate. It is applicable to detect dicyclanil in original food.
Study on Chinese Medicine Disinfectant Sterilization Effect
WANG Jin-he;BIAN Chuan-zhou;JIN Deng-yu;CHEN Yi
2010, 37(11):  227-229. 
Abstract ( 1504 )  
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In order to study the sterilization effect of the Chinese medicine disinfectant, prepared rhubarb, mugwort and other herbs as compound disinfectant in laboratory，and quantitative bactericidal test, on-site disinfection tests and clinical experimental study were carried out. The results showed that, Chinese herbal disinfectant against Staphylococcusaureus for 10 min, the killing rate was 99.9%, Sterilization for 30 min, the killing rate was 100%; more than 10 min to Escherichia coli，the killing rate was 100%; more than 15 min to Bacillus subtilis, the killing rate was 99.96%; the killing rate of on-site disinfection bacterial was 99.96%;placed in the 54 ℃ for 14 days, sterilization effect was essentially the same, presence of organic matter had a certain influence to the disinfection effect. The results suggested that, Chinese herbal disinfectant was stable and effective, the development of disinfectant was practicable.
Study on the Preparation and Drug Release of Tilmicosin Starch Microsphere Inclusion Compound
LI Zhong-jin;YANG Wei;LIU Yan;ZHAO Yan;XIONG Yong
2010, 37(11):  230-234. 
Abstract ( 1160 )  
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Tilmicosin starch microsphere were synthesized with soluble starch as material and N，N′-methylene-bis-acrylamide as crosslinking agent by using pack cementation method. The preparation process was optimized through the L9(34) orthogonal experimental design and regression analysis based on the evaluation indicators of the drug loading and encapsulation efficiency. Tilmicosin starch microsphere inclusion compound was characterized by laser particle analyzer，scan electron microscope and thermogravimetric analysis (TGA). The results indicated the best technological conditions was that: the quantity of soluble starch，tilmicosin，N，N′-methylene-bis-acrylamide and surfactant was
4，0.02，0.95，0.75 g respectively and the reacting time was 1 h. Influence factors were the quantity of N，N′-methylene-bis-acrylamide，the quantity ratio of tilmicosin to soluble starch，the reacting time，the quantity of emulsifier in order. The drug loading and embedding ratio were respective 2.24% and 89.6% by optimizing conditions. The retarded release could be observed and this preparation method was reasonable and feasible.