猬迭宫绦虫线粒体cox3基因的克隆及序列分析

Abstract

Abstract: The objectives of the present study was to examine sequence variation in the mitochondrial cytochrome c oxidase subunit 3 (cox3) gene among Spirometra erinaceieuropaei isolates from Hunan province. The partial cox3 (pcox3) was amplified from each S. erinaceieuropaei sample by PCR, and pcox3 sequences were aligned using the ClustalX 1.81. MP tree was constructed using the software Phylip 3.67 version 4.0. Sequence homology analysis was performed using the Megalign program of the software DNAStar 5.0 by comparing with the corresponding sequence of S. erinaceieuropaei available in GenBank. The results showed that the lengths of pcox3 were 264 bp. The phylogenetic tree showed that the Hunan isolates and the S. erinaceieuropaei available in GenBank were clustered in the same clade. The pcox3 sequences of S. erinaceieuropaei isolates from Hunan province were slight different. The results provided a foundation for further studies of molecular identification and molecular genetics of S.erinaceieuropaei.

Key words: Spirometra erinaceieuropaei; mitochondrial DNA; cox3 gene; sequence analysis

CLC Number:

WU Hui-lan. Cloning and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 3 of Spirometra erinaceieuropaei Isolates from Hunan Province [J]. , 2010, 37(11): 73-75.

[1]	DOU Junfeng, WANG Zui, LI Li, LU Qin, JIN Xinxin, LING Xiaochun, LUO Qingping, ZHAI Xinguo. Identification of Subgroups A, B and J Avian Leukosis Virus Co-infection and Sequence Analysis of gp85 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 302-311.
[2]	KANG Danju, PENG Qiuyun, ZHOU Jiaxin, CHEN Jinjun, LIN Hongying, ZHOU Guangxian. Cloning, Bioinformatics Analysis and Tissue Expression of AvBD1 and AvBD2 Genes in Yellow-feather Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4325-4337.
[3]	XING Tianqi, XU Di, YAN Gang, WANG Yubin, WANG Mengying, LIANG Jing, LAN Ganqiu. Sequence Analysis, Eukaryotic Expression Vector Construction and Tissue Expression of ABCD4 Gene in Bama Miniature Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4338-4348.
[4]	LI Nan, MENG Jinxin, WANG Jiannie, HE Yuwen, YANG Shaochang, LYU Shunyan, WANG Jinglin. Detection of Lumpy Skin Disease Virus in Cattle and Investigation of Virus Carried by Midges and Mosquitoes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4179-4187.
[5]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[6]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[7]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[8]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[9]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[10]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[11]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[12]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[13]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[14]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[15]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.

Cloning and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 3 of Spirometra erinaceieuropaei Isolates from Hunan Province

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments