Abstract: In vitro fertilization (IVF) technique has been maturity and more perfect, so it had setting solid foundation for the development and industrialization in cow in vitro -propagation. In this study, pregnancy rate and calf sex ratio of the non-sex control IVF embryos with artificial insemination were compared, and it was found that pregnancy rate was same between two groups and the proportion of male in the IVF group was higher than internal fertilized group. Selecting the sex control and non-sex control seminal fluid to carry out IVF test, followed by molecular identification of cow SRY gene for the non-sex control and the sex control IVF embryos. The results showed that the male proportion of sex control IVF embryos was obviously lower than the non-sex control group. In addition, the authors also compared the blastocyst rate of the sex control with non-sex control IVF embryos, but there was no-remarkable difference between the two groups. It demonstrated that sex control IVF could be used to accelerate the industrialization of high-quality cow embryos. This study encouraged that sex control IVF embryos propagation will be the applied prospect of improving dairy industry, and realize the sex control embryos industrialization.

Key words: cow in vitro-propagation technique; in vitro fertilization; embryo transplantation; sex control