Abstract: In order to explore the mechanism of Taihu goose pituitary specific transcription factor 1 (Pit1) gene transcription regulation by studying the promoter region of goose Pit1 gene，the 5′upstream sequence of Taihu goose Pit1 gene was attained by Genome Walking PCR, sequencing and sequences alignment analysis. A 3.0 kb upstream sequence of Pit1 gene was cloned firstly by Genome Walking from goose genomic template and sequenced. The sequence analysis showed that the cloned region contained two promoters， and had typical TATA box and CAAT box， but GC box was not found. Many potential transcription factors binding sits were identified by TESS analysis， which might be regulate transcription and expression the Pit1 gene potentially. The alignment results showed that the conservative region spanned from transcription start site to -200 bp of upstream，the sequence was strongly conservative and it was potential core promoter. Meanwhile，goose and chicken had higher conservative. The results showed that the cloned sequence may be considered as the promoter region of Taihu goose Pit1 gene.

Key words: goose; Pit1; Genome Walking; gene cloning; sequence analysis