小鹅瘟病毒、鹅星状病毒Ⅰ型与鸭疫里默氏杆菌混合感染的诊断及病原分析

doi:10.16431/j.cnki.1671-7236.2024.03.030

Abstract

Abstract: 【Objective】 In April 2023,an acute outbreak of infectious disease in goslings with mortality up to 25% occurred in a commercial goose farm in Xinxiang city of Henan province.To determine the potential causative agent of this disease,anatomical examination and laboratory pathogen diagnosis of dead goslings were performed.【Method】 The tissue samples of livers,spleen,kidneys,small intestine and cardiac blood and fibrous exudate from the outer layer of the liver were collected.Bacterial isolation and cultivation,Gram staining microscopy observation,16S rRNA gene identification and drug sensitivity testing were performed on samples of cardiac blood and fibrous exudate from the outer layer of the liver to determine the bacterial infection and drug sensitivity of infected geese. PCR/RT-PCR method was used to test the nucleic acid of common gosling viral infectious disease pathogens,and sequencing analysis was performed on the main structural protein genes of positive pathogens to determine the viral pathogens and their molecular epidemiology in infected geese.【Result】 In the bacterial test,the results showed that the isolated bacteria could grow as the morphology of translucent,neat edges,smooth colonies on blood agar medium.Gram-negative single and paired bacteria could be observed after Gram staining,which were consistent with the characteristics of Riemerella anatipestifer (RA).PCR amplification of 16S rRNA gene and BLAST analysis were further confirmed the isolate was RA.The drug sensitivity test revealed that the RA strain was highly sensitive to ceftriaxone and cefotaxime,and resistance to amoxicillin,tetracycline and polymyxin B. PCR and RT-PCR detection results for the common pathogens of goslings showed that all the samples were positive for Goose parvovirus (GPV) and genotype Ⅰ Goose astrovirus (GAstV-1), and no corresponding nucleotide fragments were observed for GAstV-2,Avian influenza virus (AIV) and Goose reovirus (GRV). The two identified virus strains were designated as GPV/HN-2023 and GAstV-1/HN-2023,respectively.Furthermore,the analysis of the main structral protein gene of GPV/HN-2023 and GAstV-1/HN-2023 revealed that GPV/HN-2023 was closely related to DY-16 strain and belonged to DY-16-like strain,with two unique amino acid mutations D248E and V314L in VP3 protein.GAstV-1/HN-2023 was closely related to GAstV-1 strains and grouped into GAstV-1 branch,with three unique amino acid mutations G47R,S207G and A628T in ORF2 protein.【Conclusion】 This study identified the mixed infection of GPV,GAstV-1,and RA as the cause of gosling disease in the goose farm through comprehensive diagnostic methods.The drug resistance of pathogenic bacteria RA and the genetic evolution and variation characteristics of viral pathogens GPV and GAstV-1 were analyzed,providing theoretical reference for the scientific prevention and control of gosling disease in Henan region.

Key words: Goose parvovirus (GPV); genotypeⅠ Goose astrovirus (GAstV-1); Riemerella anatipestifer; co-infection; etiology analysis

CLC Number:

S852.6

ZHANG Xiaozhan, XING Zhongyu, LYU Nannan, DONG Xuanzhi, LI Yulin, MAO Tianji, LIANG Yaohan, GUO Yunze, SONG Yuzhen, QIAO Hongxing, BIAN Chuanzhou, YUAN Ye, LIANG Qunchao. Diagnosis and Pathogen Analysis of Co-infection of Goose Parvovirus, GenotypeⅠ Goose Astrovirus and Riemerella anatipestifer[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1183-1193.

References

[1] 侯水生,刘灵芝.2022年水禽产业现状、未来发展趋势与建议[J].中国畜牧杂志, 2023, 59(3):274-280. HOU S S, LIU L Z.Development status, future development trends and suggestions of waterfowl industry in 2022[J]. Chinese Journal of Animal Science, 2023, 59(3):274-280.(in Chinese)
[2] 刘月焕.加快推进我国水禽疫病防控技术的研究[J].中国农业科学, 2019, 52(23):4386-4389. LIU Y H.Promoting the research on prevention and control of waterfowl infectious disease in China[J].Scientia Agricultura Sinica, 2019, 52(23):4386-4389.(in Chinese)
[3] 黄远丕,黄运茂,闫俊书,等.饲粮因素诱发雏鹅痛风的机制剖析及营养调控技术[J].动物营养学报, 2020, 32(9):4029-4036. HUANG Y P, HUANG Y M, YAN J S, et al.Mechanism analysis and nutritional regulation technology of gosling gout induced by dietary factors[J].Chinese Journal of Animal Nutrition, 2020, 32(9):4029-4036.(in Chinese)
[4] 王志,胡仲皓,李思婷,等.高蛋白日粮诱发雏鹅高尿酸血症的血清代谢组学研究[J].南京农业大学学报, 2021, 44(6):1169-1176. WANG Z, HU Z H, LI S T, et al.Study on serum metabonomics of goslings with hyperuricemia induced by high protein diet[J].Journal of Nanjing Agricultural University, 2021, 44(6):1169-1176.(in Chinese)
[5] ZHANG X, DENG T, SONG Y, et al.Identification and genomic characterization of emerging Goose astrovirus in Central China, 2020[J].Transboundary and Emerging Diseases, 2022, 69(3):1046-1055.
[6] HUANG Y, ZHANG J, DONG J, et al.Isolation and characterization of a new Goose orthoreovirus causing liver and spleen focal necrosis in geese, China[J].Transboundary and Emerging Diseases, 2022, 69(5):3028-3034.
[7] 张秀芬.山东省部分地区雏鹅早期死亡原因的流行病学调查[D].泰安:山东农业大学, 2017. ZHANG X F.Epidemiological investigation of early death in goslings in Shandong province of China[D].Tai'an:Shandong Agricultural University, 2017.(in Chinese)
[8] TSAI H J, LIU Y T, TSENG C S, et al.Genetic variation of the ompA and 16S rRNA genes of Riemerella anatipestifer[J].Avian Pathology, 2005, 34(1):55-64.
[9] 刘金华,甘孟侯.中国禽病学[M].2版.北京:中国农业出版社, 2016. LIU J H, GAN M H.China Poultry Diseases[M].2nd ed.Beijing:China Agriculture Press, 2016.(in Chinese)
[10] YAO M, ZHANG X, GAO Y, et al.Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks[J].BMC Veterinary Research, 2019, 15(1):103.
[11] ZHANG X, REN D, LI T, et al.An emerging novel Goose astrovirus associated with gosling gout disease, China[J].Emerging Microbes&Infections, 2018, 7(1):152.
[12] NATIONAL COMMITTEE FOR CLINICAL LABORATORY STANDARDS.Performance standard for antimicrobial susceptibility testing;Twentieth informational supplement[S].National Committee for Clinical Laboratory Standards, 2010, M100-S20.
[13] KUMAR S, STECHER G, TAMURA K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for Bigger Datasets[J].Molecular Biology and Evolution, 2016, 33(7):1870-1874.
[14] 刘国平,王齐.科学发展肉鹅养殖业,助力乡村振兴[J].中国动物保健, 2023, 25(4):107-108. LIU G P, WANG Q.Scientific development of meat goose breeding industry to help rural revitalization[J].China Animal Health, 2023, 25(4):107-108.(in Chinese)
[15] 贾婧宇,凌珏怡,王志仙,等.鹅细小病毒DY16株的分子鉴定及重组分析[J].中国兽医学报, 2019, 39(5):848-852. JIA J Y, LING Y Y, WANG Z X, et al.Molecular characterization and recombination analysis of a Goose parvovirus isolate DY16[J].Chinese Journal of Veterinary Science, 2019, 39(5):848-852.(in Chinese)
[16] LIU P, ZHANG J, CHEN S, et al.Genome sequence of a Goose parvovirus strain isolated from an ill goose in China[J].Genome Announcements, 2017, 5(17):e00227-17.
[17] 张敏.我国部分地区规模化鹅场五种重要病毒病的流行病学调查[D].泰安:山东农业大学, 2016. ZHANG M.Epidemiological investigation of five important virus diseases on large-scale goose farms in some regions in China[D].Tai'an:Shandong Agricultural University, 2016.(in Chinese)
[18] HE D, WANG F, ZHAO L, et al.Epidemiological investigation of infectious diseases in geese on mainland China during 2018-2021[J].Transboundary and Emerging Diseases, 2022, 69(6):3419-3432.
[19] LIU H, HU D, ZHU Y, et al.Coinfection of Parvovirus and Astrovirus in gout-affected goslings[J].Transboundary and Emerging Diseases, 2020, 67(6):2830-2838.
[20] 陈栋,张言浩,张阳,等.3株鸭疫里氏杆菌的分离鉴定与药敏试验[J].中国畜牧兽医, 2023, 50(3):1250-1258. CHEN D, ZHANG Y H, ZHANG Y, et al.Isolation, identification and drug sensitivity of 3 strains of Riemerella anatipestifer[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(3):1250-1258.(in Chinese)
[21] 雍燕,刘国昌,方炳虎,等.不同地区鸭疫里氏杆菌的分离鉴定与药敏试验[J].中国畜牧兽医, 2017, 44(1):242-247. YONG Y, LIU G C, FANG B H, et al.Isolation, identification and drug-sensitivity test of Riemerella anatipestifer from different areas[J].China Animal Husbandry&Veterinary Medicine, 2017, 44(1):242-247.(in Chinese)
[22] 祝希辉,李艳兰,王志伟,等.1株鸭疫里默氏杆菌的全基因组测序及耐药性与遗传进化分析[J].中国畜牧兽医, 2023, 50(3):1140-1149. ZHU X H, LI Y L, WANG Z W, et al.Whole genome sequencing of a resistant Riemerella anatipestifer strain and analysis of antibiotic resistance and genetic evolution[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(3):1140-1149.(in Chinese)
[23] CHEN Y, AFUMBA R, PANG F, et al.Advances in research on genetic relationships of waterfowl Parvoviruses[J].Journal of Veterinary Research, 2021, 65(4):391-399.

Diagnosis and Pathogen Analysis of Co-infection of Goose Parvovirus, GenotypeⅠ Goose Astrovirus and Riemerella anatipestifer

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.
[2]	CHENG Longfei, LIAO Weilian, CHEN Hongmei, JIANG Nansong, FU Qiuling, WAN Chunhe, LIU Rongchang, SHI Shaohua, HUANG Yu, FU Guanghua. Screening and Biological Characteristics of Broad-spectrum Riemerella anatipestifer Phage [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1169-1176.
[3]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[4]	LIU He, LI Qiong, HUANG Jingjing, HU Xiaoyang, WANG Xiaoyan, ZHAO Hui. Isolation and Identification of Pathogenic Bacteria Associated with Neurological Signs of Jinding Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5114-5122.
[5]	YANG Yunyun, ZHANG Qiandong, YUAN Yang, WANG Wei, TIAN Qin, CAI Haiqing, LIU Xin, WEN Guilan, YANG Ying, CHENG Zhentao, WEN Ming. Metabolomic Analysis of Ileum Co-infected with Duck Enteritis Virus and Clostridium perfringens Type A in Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4759-4767.
[6]	XU Jinge, LI Ting, CHEN Rujia, YU Bo, ZHANG Yanan, YANG Li, ZHAO Bin, PU Ling. Preparation and Immune Efficacy of Riemerella anatipestifer Bivalent Inactivated Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 290-299.
[7]	MA Guangqiang, LIU Lijuan, CHEN Guoquan, WU Liangtao, YANG Jun, LIU Junze, LI Xiaomeng, PAN Chengwen, ZHOU Bijun. Preparation of Propolis Inactivated Vaccine of Riemerella anatipestifer Epidemic Strain in Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3163-3170.
[8]	LI Linlin, DONG Jiawen, ZHANG Junqin, HUANG Yunzhen, SUN Minhua. Construction and Main Biological Characteristics Determination of RIA_0940 Gene Deleted Strain of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4564-4572.
[9]	JIANG Ning, YIN Hang, ZHANG Yanwei, LI Zixin, CHI Xiaojuan, WANG Song. Research Progress on Co-infection of H9N2 Subtype Avian Influenza Virus with Other Pathogens [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3447-3455.
[10]	TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer Infection on the Microflora of Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2129-2139.
[11]	WU Zhengzhuo, CHEN Guoquan, YAO Biqiong, SHI Dajun, YAN Chaohua, WANG Na, WANG Kaigong, ZHOU Bijun, CHENG Zhentao, WEN Ming. Isolation, Identification, Virus Gene Detection and Antibiotic Resistance Analysis of Riemerella anatipestifer in Sansui County of Guizhou Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4274-4283.
[12]	TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer on the Morphological Structure and TLR4 Signal Pathway Related Gene Expression of Cecum in Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1702-1708.
[13]	XIAO Kun, CHEN Guang, ZHANG Jun, WEN Guilan, WEN Ming, CHENG Zhentao. Isolation,Identification and Drug Resistance Analysis of Riemerella anatipestifer from Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4085-4092.
[14]	DENG Tongwei, XUE Yanan, LU Jianzhou, XIA Yanxun, CHEN Lulu, GUO Yiwen, PENG Liming, JIANG Zenghai, QIAO Hongxing, XU Yaohui, ZHANG Xiaozhan. Diagnosis and Pathogen Analysis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus,Porcine Circovirus Type 2 and Haemophilus parasuis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3401-3409.
[15]	DAI Yin, ZHANG Danjun, ZHAO Ruihong, HU Xiaomiao, SHEN Xuehuai, HOU Hongyan, PAN Xiaocheng, ZHOU Xueli. Cloning and Sequence Analysis of NS and VP Genes of Goose Parvovirus Strain in Anhui [J]. , 2018, 45(5): 1177-1183.