China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (11): 4759-4767.doi: 10.16431/j.cnki.1671-7236.2023.11.042

• Basic Veterinary Medicine • Previous Articles     Next Articles

Metabolomic Analysis of Ileum Co-infected with Duck Enteritis Virus and Clostridium perfringens Type A in Ducks

YANG Yunyun1, ZHANG Qiandong1, YUAN Yang1, WANG Wei1, TIAN Qin3, CAI Haiqing1, LIU Xin1, WEN Guilan1,2, YANG Ying1,2, CHENG Zhentao1,2, WEN Ming1,2   

  1. 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. Guizhou Engineering Research Center of Animal Biological Products, Guiyang 550025, China;
    3. Agriculture and Rural Bureau of Xixiu District, Anshun 561000, China
  • Received:2023-04-23 Online:2023-11-05 Published:2023-10-27

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Abstract: 【Objective】 The purpose of this study was to investigate the pathogenic mechanism of Duck enteritis virus (DEV) and Clostridium perfringens type A (CpA) co-infection.【Method】 Healthy non-immune ducklings at 37 days of age were randomly divided into control and co-infected groups.The co-infected group were gavaged once a day in the morning and evening, with 8 mL of CpA bacterial solution (1×108 CFU/mL) for 5 consecutive days, and after 72 h of initial gavage, the DEV-GZ strain virus solution (0.2 mL, TCID50=3.16×10-9 TCID50/0.1 mL) was inoculated into the leg muscles, and control group was inoculated with sterilized saline 0.2 mL.The clinical lesions and ileal dissection lesions of the ducks were observed.Bacterial isolation and identification and PCR were used to detect whether the co-infection model was successfully established by DEV and CpA.The ileum of control and co-infected ducks was collected at 90 h of infection, and liquid chromatography-mass spectrometry (LC-MS) was used for metabolomic analysis of the ileum of control and co-infected ducks at 90 h of infection to screen and analyze potential differential metabolites and related signaling pathways.【Result】 A duck DEV and CpA co-infection model was successfully constructed after testing. A total of 36 different metabolites were found in the ileum of ducks in control and co-infected groups.16 different metabolites were found in the positive ionization mode, mainly including 2'-deoxyinosine, putrescine, 8(z), 11(z), 14(z)-eicosatrienoic acid, cytidine, and 1-oleoyl racemic glycerol, and 20 different metabolites were found in the negative ionization mode, mainly including oleic acid, arachidonic acid, 11(z), 14(z)-docosa-2-dienoic acid, docosa-2-trienoic acid and linoleic acid.The main metabolic pathways enriched in differential metabolites were 10, mainly tryptophan metabolism, pyrimidine metabolism, purine metabolism and so on.【Conclusion】 11(z), 14(z)-docosadienoic acid, docosatrienoic acid, and arachidonic acid could be sensitive biomarkers in the ileum of ducks co-infected with DEV and CpA, suggesting that the co-infection of DEV and CpA exacerbated the inflammation of the ileum in ducklings, and that tryptophan metabolism pathway suggested a link between the changes in the immunity in ducks co-infected with DEV and CpA.This study laid a foundation for elucidating the pathogenic mechanism of DEV and CpA infection.

Key words: DEV; CpA; co-infection; metabonomics

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