鸡环状RNA circWWP1的鉴定及组织表达研究

doi:10.16431/j.cnki.1671-7236.2023.10.005

Abstract

Abstract: 【Objective】 The purpose of the experiment was to identify the expression of circular RNA (circRNA) WW domain-containing E3 ubiquitin protein ligase 1 (circWWP1) in chickens,and to further explore its tissue expression characteristics,its role in chicken muscle growth and development and its possible mechanism,so as to lay a foundation for the study of the regulatory mechanism of circWWP1 on chicken muscle development.【Method】 The authenticity and stability of circWWP1 were determined by PCR amplification,DNA sequencing,RNase R enzyme digestion and actinomycin D treatment,and the tissue expression characteristics of circWWP1 were detected by Real-time quantitative PCR.The circWWP1 miRNA binding site and its target genes were predicted by online software.The circWWP1-miRNA-mRNA network was constructed by Cytoscape 3.9.1 software,and GO function and KEGG pathway enrichment analysis were performed.【Result】 circWWP1 was present and stably expressed in chickens,which were resistant to RNase R enzyme digestion and actinomycin D treatment.circWWP1 was widely expressed in all tissues of adult chickens,and the expression level in breast muscle was significantly higher than that in other tissues (P＜0.05).It was only expressed in breast muscle and leg muscle of 22-day-old chicks.The expression level of circWWP1 in breast muscle and leg muscle of adult chickens was significantly higher than that of 22-day-old chicks (P＜0.05).The analysis of circWWP1-miRNA-mRNA network regulation obtained three target miRNAs (gga-miR-454-3p,gga-miR-130a-3p and gga-miR-301b-3p) and their downstream targets of 208 genes.GO function enrichment showed that the circWWP1 target genes were significantly enriched in 38 GO items.KEGG pathway enrichment analysis showed that circWWP1 target genes were mainly involved in Hedgehog signaling pathway related to cell proliferation and embryonic development.【Conclusion】 circWWP1 existed and was stably expressed in chickens,and was highly expressed in breast muscle and leg muscle tissues of chickens at different ages,which might play a role in chick embryo development and muscle cell proliferation and differentiation through Hedgehog signaling pathway.

Key words: chicken; circRNA; circWWP1; tissue expression; muscle development

CLC Number:

S831

YANG Yaling, SHEN Xuemei, YU Shigang, WANG Gang, GULIPARI Aikebai, LIU Wujun. Identification and Tissue Expression of Circular RNA circWWP1 in Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 3917-3927.

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Identification and Tissue Expression of Circular RNA circWWP1 in Chicken

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