牛病毒性腹泻病毒LAMP检测方法的建立与应用

doi:10.16431/j.cnki.1671-7236.2015.12.001

Abstract

Abstract: Since the 5'UTR gene (GenBank No.:AY278459.1) had 4 isolated regions,we designed a set of 4 LAMP primers to specifically recognize target gene sequences.This study developed a loop-mediated isothermal amplification (LAMP) method for detecting BVDV,using the pyrophosphate magnesium white precipitate for Real-time detection in LAMP reaction process of turbidity instrument,Real-time monitor liquid turbidity to determine result.The whole reaction lasted only 50 minutes at a constructed temperature of 63 ℃ to evaluate specificity,sensibility and repeatability of the method.The result demonstrated that the LAMP assay could only react with BVDV,its specificity was high;It could detect at least 10^-6-fold diluted samples,which was 100 more sensitive than PCR assay;And repeatability was good.The simple,rapid,high siensitivity and specificity LAMP assay was a potential tool for the detection of BVDV in field conditions.

Key words: bovine viral diarrhea virus (BVDV); loop-mediated isothermal amplification method (LAMP); PCR

CLC Number:

LI Jia-wei, GUO Li, YANG Yong, WANG Jian-ke, ZHANG Shu-qin, ZHANG Jia-li, CHENG Shi-peng. Establishment and Application of LAMP Detection Method of Bovine Viral Diarrhea Virus[J]. , 2015, 42(12): 3111-3118.

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Establishment and Application of LAMP Detection Method of Bovine Viral Diarrhea Virus

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