基于LC-MS/MS分析板芪清肺组方发酵代谢差异

doi:10.16431/j.cnki.1671-7236.2023.10.038

Abstract

Abstract: 【Objective】 The experiment aimed to explore the metabolic differences of traditional Chinese medicine in the fermentation of Banqi Qingfei prescription by Bacillus subtilis at the metabolomics level.【Method】 Using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) technology,multiple statistical analysis and metabolic pathway analysis such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA),the differential metabolites and metabolic pathways between the traditional Chinese medicine powder of Banqi Qingfei prescription and the traditional Chinese medicine powder of Banqi Qingfei prescription fermented by Bacillus subtilis were explored.【Result】 A total of 34 624 differential metabolites were screened out in the fermentation group of Banqi Qingfei granules (Enzyme group) and the control group of unfermented Banqi Qingfei granules (Water group) in the positive ion mode,and 15 762 differential metabolites were screened out in the negative ion mode.Cluster analysis was conducted on the differential metabolites,and the identified differential metabolites could be divided into 203 categories.The PCA diagram of the relationship between the positive and negative ion mode groups showed good differentiation effect.After fermentation,the main up-regulated differential metabolites include equol,ethynodiol diacetate,vanylglycol,D-ornithine,quinestrol,cholesterol and acetylcholine,and the down-regulated differential metabolites include Aspergillus flavus,erucic acid,myristic acid and xanthine.After enrichment analysis of KEGG metabolic pathways,27 significantly different metabolic pathways were screened,including proximal tubule bicarbonate recovery,arginine biosynthesis,biosynthesis of histidine and purine derived alkaloids,biosynthesis of plant secondary metabolites,phenylpropane biosynthesis,protein digestion and absorption,etc.【Conclusion】 After the fermentation of Banqi Qingfei prescription,amino acids such as lipids and ketones of differential metabolites increased significantly,and acid amino acids decreased significantly,involving multiple amino acid biosynthesis and purine metabolism,providing a theoretical basis for the fermentation products of Banqi Qingfei prescription.

Key words: Banqi Qingfei prescription; fermentation; LC-MS/MS; metabonomics

CLC Number:

S853.7

ZHANG Yan, LIU Xuesong, XUE Zhanmei, YANG Haotian, ZHANG Guohua, ZHANG Bei, LI Li, FENG Wanyu, WANG Zhiqiang, ZHONG Peng, SHI Tongrui. Analysis of Difference in Fermentation Metabolism of Banqi Qingfei Prescription Based on LC-MS/MS[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4243-4251.

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Analysis of Difference in Fermentation Metabolism of Banqi Qingfei Prescription Based on LC-MS/MS

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