金定鸭神经症状相关致病菌分离和鉴定

doi:10.16431/j.cnki.1671-7236.2023.12.033

Abstract

Abstract: 【Objective】 The aim of the present study was to determine the pathogenic bacteria responsible for neurologic signs of Jinding ducks.【Method】 Twenty-two sick ducks,which displayed neurologic signs and lesions of fibrinous pericarditis,perihepatitis and airsacculitis,were randomly selected from four 24- to 48-day-old flocks of Jinding ducks from a region with intensive egg-laying duck production in Liaoning province.Brain tissues were sampled and bacterial isolation were performed by using tryptic soy agar (TSA) and MacConkey’s agar (MCA),and identified by Gram and Wright’s staining,biochemical testing,and 16S rDNA gene sequencing.【Result】 The 19 strains of bacteria isolated from the brain tissue of Jinding duck were all Gram-negative bacteria.Among them,17 (named H1-H17) failed to grow in MCA and tested negative in the biochemical assays,whereas other 2 isolates (named H18 and H19) formed red colonies in MCA,fermented glucose,lactose,and maltose,and were methyl red and indole positive.On the basis of comparative analyses of the 16S rDNA amplicon sequences,the 17 isolates represented by H1 shared 99.1%-100% identities with reference strains of RA serotypes 1 to 19.The H18 and H19 isolates were 99.2%-99.9% identical to the selected eight reference Escherichia coli strains.Phylogenetic analysis based on the amplified 16S rDNA amplicon sequences further demonstrated that the 17 isolates represented by H1 belonged to RA and that isolates H18 and H19 belonged to Escherichia coli.【Conclusion】 In the studied cases,RA was responsible for neurologic signs in Jinding ducks,and that Escherichia coli could also cause neurologic signs in Jinding ducks.

Key words: Jinding duck; Riemerella anatipestifer; Escherichia coli; neurologic signs

CLC Number:

S855.1

LIU He, LI Qiong, HUANG Jingjing, HU Xiaoyang, WANG Xiaoyan, ZHAO Hui. Isolation and Identification of Pathogenic Bacteria Associated with Neurological Signs of Jinding Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5114-5122.

References

[1] 张大丙.鸭病图鉴[M].北京:中国农业科学技术出版社,2019. ZHANG D B.Illustration of Duck Disease[M].Beijing:China Agriculture Science and Technology Press,2019.(in Chinese)
[2] WEI B,CHA S Y,KANG M,et al.Development and application of a multiplex PCR assay for rapid detection of 4 major bacterial pathogens in ducks[J].Poultry Science,2013,92:1164-1170.
[3] 郭玉璞,陈德威,范国雄,等北京小鸭传染性浆膜炎的调查研究[J].畜牧兽医学报,1982,13(2):107-112. GUO Y P,CHEN D W,FAN G X,et al.Studies on infections serositis in white Beijing ducklings[J].Acta Veterinarial et Zootechnica Sinica,1982,13(2):107-112.(in Chinese)
[4] 马芹,汪建华,张梦亚,等.一株致种鸭瘸腿的肠炎沙门氏菌分离与鉴定[J].中国家禽,2022,44(12):40-45. MA Q,WANG J H,ZHANG M Y,et al.Isolation and identification of a strain of Salmonella Enteritidis isolated from lame breeder duck[J]. China Poultry,2022,44(12):40-45.(in Chinese)
[5] JORTNER B S,PORRO R,LEIBOVITZ L.Central-nervous-system lesions of spontaneous Pasteurella anatipestifer infection in ducklings[J].Avian Diseases,1969,13(1):27-35.
[6] DAVID E S.Diseases of Poultry[M].14th edition.New Jersey:John Wiley&Sons,Incorporated,2020.
[7] 郭玉璞,蒋金书.鸭病[M].北京:北京农业大学出社,1988. GUO Y P,JIANG J S.Duck Diseases[M].Beijing:Beijing Agriculture University Press,1988.(in Chinese)
[8] CHIKUBA T,UEHARA H,FUMIKURA S,et al.Riemerella anatipestifer infection in domestic ducks in Japan,2014[J].Journal of Veterinary Medical Science,2016,78(10):1635-1638.
[9] 温俊板,李永明,周碧君,等.以神经症状为主征的鸡白痢沙门氏菌病[J].西南农业学报,1992,3:116-118. WENG J B,LI Y M,ZHOU B J,et al. Salmonella Pullorum disease characterized by nervous symptom[J].Southwest China Journal of Agriculture Sciences,1993,3:116-118.(in Chinese)
[10] 刘富来,冯翠兰,王林川.鸭肠炎沙门氏菌分离鉴定与药敏试验[J].动物医学进展,2004,3:76-80. LIU F L,FENG C L,WANG L C.Isolation,identification and drug sensitive test of Salmonella Enteritidis in duck[J]. Progress in Veterinary Medicine,2004,3:76-80.(in Chinese)
[11] 费恩阁,李德昌,丁壮.动物疫病学[M].北京:中国农业出版社,2004. FEI E G,LI D C,DING Z.Animal Epidemiology[M].Beijing:Beijing Agriculture Press,2004.(in Chinese)
[12] LE GUENNEC L,COUREUIL M,NASSIF X,et al.Strategies used by bacterial pathogens to cross the blood-brain barrier[J].Cell Microbiology,2020,22(1):e13132.
[13] LI Z,BRUNO V M,KIM K S.Central nervous system-infecting pathogens Escherichia coli and cryptococcus neoformans exploit the Host Pdlim2 for intracellular traversal and exocytosis in the blood-brain barrier[J].Infection and Immunity,2021,89(10):e0012821.
[14] NEUWELT E A.Mechanisms of disease:The blood-brain barrier[J]. Neurosurgery,2004,54(1):131-140.
[15] WANG L,PAN F,GENG J.Inhibition of Escherichia coli induced bacterial meningitis by ansamycin loaded polymeric nanoparticles[J].Indian Journal of Pharmaceutical Education and Research,2022,56(4):976-983.
[16] ONO M,TANAKA M.Sanitization of a duck house contaminated with Moraxella (Pasteurella) anatipestifer[J]. Journal of the Japanese Society for Food Science and Technology-nippon Shokuhin,1988,24:138-141.
[17] 曲丰发.依据16S rRNA基因和16S-23S rRNA基因间隔区对鸭疫里默氏菌进行系统发育分析[D].北京:中国农业大学,2006. QU F F.Phylogenetic analysis of Riemerella anatipestifer based on 16S rRNA gene and 16S-23S rRNA intergenic spacer region[D].Beijing:China Agriculture University,2006.(in Chinese)
[18] 周煜.16S rRNA序列分析法在医学微生物鉴定中的应用[J].生物技术通讯,1999,10(4):297-305. ZHOU Y.Application of 16S ribosomal RNA approach for the identification of medical microbe[J].Letters in Biotechnology,1999,10(4):297-305.(in Chinese)
[19] 刘伯承,王慧,刘俊琦,等.牛源肠聚集性大肠杆菌的分离鉴定[J].中国兽医杂志,2022,58(6):45-51. LIU B C,WANG H,LIU J Q,et al.Isolation and identification of bovine-originated enteroaggregative Escherichia coli[J].Chinese Journal of Veterinary Medicine,2022,58(6):45-51.(in Chinese)
[20] 董洪燕,宋海港,朱善元,等.鸭源大肠杆菌的分离鉴定及致病性分析[J].中国畜牧兽医,2022,49(5):1977-1984. DONG H Y,SONG H G,ZHU S Y,et al.Isolation,identification and pathogenicity analysis of Escherichia coli from ducks[J].China Animal Husbandry&Veterinary Medicine,2022,49(5):1977-1984.(in Chinese)
[21] SEGER P,MNNHEIM W,VANCANNEYT M,et al. Riemerella anatipestifer gen.nov.,comb.nov.,the causitive agent of septicemia anserum exsudativa,and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group[J].Journal of Systematic Bacteriology,1993,43:768-776.
[22] 刘朝军,沈定霞.16S rDNA序列测定在细菌鉴定中的应用[J].军医进修学院学报,2011,32(7):774-776. LIU C J,SHEN D X.Application of 16S rDNA sequencing for identification of bacteria[J].Journal of Chinese PLA Postgraduate Medical School,2011,32(7):774-776.(in Chinese)
[23] 詹銮峰,高鹏.16S rDNA检测在临床细菌检验中的应用[J].中国实验诊断学,2011,15(7):1140-1142. ZHAN L F,GAO P.Application of 16S rDNA for the detection of clinical bacteria[J]. Chinese Journal of Laboratory Diagnosis,2011,15(7):1140-1142.(in Chinese)
[24] 方光远,茅慧华,蒋加进,等.不同病例动物源大肠杆菌的分离鉴定及16S rDNA同源性分析[J].中国畜牧兽医,2015,42(12):3126-3132. FANG G Y,MAO H H,JIANG J J,et al.Isolation,identification and 16S rDNA homology from animals in different analysis of Escherichia coli cases[J].China Animal Husbandry&Veterinary Medicine,2015,42(12):3126-3132.(in Chinese)
[25] SUBRAMANIAM S,CHUA K L,TAN H M,et al.Phylogenetic position of Riemerella anatipestifer based on 16S rRNA gene sequences[J].International Journal of Systematic Bacteriology, 1997,47:562-565.
[26] 邹荣华,吴晓妮,陈启伟,等.Enolase在鸭疫里默氏杆菌侵袭鸭脑微血管内皮细胞中的作用[J].畜牧兽医学报,2022,53(12):4389-4397. ZOU R H,WU X N,CHEN Q W,et al.Effect of enolase on Riemerella anatipestifer invading duck brain microvascular endothelial cells[J]. Acta Veterinaria et Zootechnica Sinica,2022,53(12):4389-4397.(in Chinese)
[27] 冯雅婷,朱敏,刘丹,等.鸭疫里默氏杆菌流行菌株的分离鉴定及生物学特性[J].微生物学通报,2022,11:4778-4785. FENG Y T,ZHU M,LIU D,et al.Isolation,identification,and biological characterization of Riemerella anatipestifer epidemic strains[J].Microbiology China,2022,11:4778-4785.(in Chinese)
[28] LI S,GONG X,CHEN Q, et al.Threshold level of Riemerella anatipestifer crossing blood-brain barrier and expression profiles of immune-related proteins in blood and brain tissue from infected ducks[J].Veterinary Immunology and Immunopathology, 2018,200:26-31.
[29] SUN J,ZHU D,XU J,et al.Rifampin resistance and its fitness cost in Riemerella anatipestifer[J].BMC Microbiology,2019,19(1):107.
[30] 刘马峰,田琇,程安春.鸭疫里默氏杆菌毒力及耐药机制研究进展[J].微生物学报,2019,59(7):1222-1231. LIU M F,TIAN X,CHENG A C.Research advances in the virulence and antibiotics resistance mechanism of Riemerella anatipestifer[J]. Acta Microbiologica Sinica,2019,59(7):1222-1231.(in Chinese)
[31] 顾庆林,包涛涛,鲜思美,等.23株鸭疫里默氏杆菌贵州株的耐药及耐消毒剂基因分析[J].中国预防兽医学报,2023,45(1):95-99. GU Q L,BAO T T,XIAN S M,et al.Gene analysis of antimicrobial resistance and disinfectant resistance of 23 Riemerella anatipestifer Guizhou strains[J]. Chinese Journal of Preventive Veterinary Medicine 2023,45(1):95-99.(in Chinese)
[32] SADIGH-ETEGHAD S,SHAHI S,MAHMOUDI J,et al.Application of nano-based drug loading systems in the treatment of neurological infections:An updated review[J].Current Pharmaceutical Design,2022,28(28):2330-2342.
[33] HERSH A M,ALOMARI S,TYLER B M.Crossing the blood-brain barrier:Advances in nanoparticle technology for drug delivery in neuro-oncology[J].International Journal of Molecular Sciences,2022,23(8):4153.
[34] VAN DE BEEK D,CABELLOS C,DZUPOVA O,et al.ESCMID guideline:Diagnosis and treatment of acute bacterial meningitis[J].Clinical Microbiology and Infection,2016,22:S37-62.

Isolation and Identification of Pathogenic Bacteria Associated with Neurological Signs of Jinding Ducks

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHU Kaiqing, ZHU Yue, LIU Yingying, CUI Yanan, JIANG Bingyu, LI Yan. Screening of Differentially Expressed cricRNA of Porcine Small Intestinal Epithelial Cells at Early ETEC infection [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3490-3498.
[2]	SUN Yue, WANG Qi, MAO Wei, WANG Bo, DONG Haiyan, FAN Hongliang, HAO Puguo, ZHAO Hongxia. Drug Resistance Analysis and Virulence Gene Detection of Escherichia coli Causing Calf Diarrhea in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3811-3822.
[3]	ZOU Hong, LUO Gan, CHEN Ling, DU Pengfei, ZHANG Qianyi, YANG Chenghuai, TIAN Ye. Research Progress on HPI Virulence Island and Its Horizontal Transfer in Pathogenic Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3430-3437.
[4]	LIU Chunyan, TANG Qingsong, YANG Bijing, WU Qiwen, XIONG Yunxia, HUANG Yanna, YANG Xuefen, WANG Li, JIANG Zongyong, YI Hongbo. Effects of Enterotoxigenic Escherichia coli on Intestinal Morphology, Tight Junction Protein and Extracellular Matrix of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2697-2706.
[5]	CHENG Qian, GAO Qingqing, WANG Yuhe, HUAN Changchao, GAO Song. Effects of Avian Pathogenic Escherichia coli Outer Membrane Protein OmpA on Autophagy of DF-1 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1971-1980.
[6]	WANG Leping, PAN Yan, TAN Yizhou, LI Lei, HAN Kaiou, CAO Yajie, LI Jun, ZHOU Yuqing, LIAO Yuying, MA Dongxin, GONG Yu, PENG Hao, WANG Xiaoye. Isolation,Identification and Genome Analysis of Escherichia coli Phage vB_EcoP_GN07 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1522-1531.
[7]	LI Cuihan, QU Zhina, GAO Yubin, LI Yan, WANG Juan, DUAN Xiaoxiao, WANG Lin, ZHANG Xiyue, ZHAO Ge, HUANG Xiumei, ZHAO Jianmei, ZHANG Qingqing, WANG Junwei, HUANG Baoxu, LIU Junhui. Epidemiology Investigation and Whole Genome Sequencing Analysis of Three Types of Poultry Originated Carbapenem-resistant Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1653-1662.
[8]	HUANG Qiaochen, LIAO Binru, YU Chunyan, MO Yupeng, HUANG Weiqian, LI Lei, HAN Kaiou, WANG Leping, LI Xun, WANG Xiaoye. Isolation,Identification and Biological Characteristics Analysis of a Bacteriophage EP_H11 from Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1684-1694.
[9]	ZHU Qiqi, ZHANG Jiyu, WANG Chengye, WU Lingyu, WANG Weiwei, ZHU Zhen. Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1081-1092.
[10]	ZHU Xihui, LI Yanlan, WANG Zhiwei, PANG Zheyu, LI Yubao, LIU Cheng, SI Zhenshu, LU Jianbiao, CAO Shengliang, XUE Xijuan. Whole Genome Sequencing of a Resistant Riemerella anatipestifer Strain and Analysis of Antibiotic Resistance and Genetic Evolution [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1140-1149.
[11]	CHENG Longfei, LIAO Weilian, CHEN Hongmei, JIANG Nansong, FU Qiuling, WAN Chunhe, LIU Rongchang, SHI Shaohua, HUANG Yu, FU Guanghua. Screening and Biological Characteristics of Broad-spectrum Riemerella anatipestifer Phage [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1169-1176.
[12]	CHEN Dong, ZHANG Yanhao, ZHANG Yang, LI Sen, ZHOU Zutao, LI Zili. Isolation,Identification and Drug Sensitivity of 3 Strains of Riemerella anatipestifer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1250-1258.
[13]	LUO Gan, ZOU Hong, REN Shaoke, WU Chunxia, ZOU Yao, LOU Yinying, ZHOU Yang, CHENG Fangjun, GUO Jianhua. Detection of O-antigen,Virulence Gene and Drug Sensitivity Test of Pathogenic Escherichia coli from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 713-721.
[14]	ZANG Jiajia, LI Bing, TENG Yi, YANG Yuzhu, PI Dejin, CHEN Qinyin, GUO Shuangshuang, LAI Yujiao, YI Dan. Effect of Puerarin on Growth Performance,Intestinal Antioxidant Capacity and Intestinal Flora in Broilers Challenged with Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4846-4857.
[15]	SHI Weidi, KANG Lichao, LIU Caixia, KOU Lijun, LYU Shuangfei, REN Huijie, QIAN Ruixuan, MA Xun, WANG Jing, JIANG Jianjun, GAO Shengjie. Isolation,Identification,Pathogenicity and Drug Resistance Analysis of Extraintestinal Pathogenic Escherichia coli from Calves [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5022-5031.