葛根素对大肠杆菌感染肉鸡生长性能和肠道抗氧化功能及菌群的影响

doi:10.16431/j.cnki.1671-7236.2023.12.007

Abstract

Abstract: 【Objective】 The experiment was conducted to study the effect of puerarin on growth performance,blood biochemical indexes,antioxidant capacity and caecum microflora of broilers challenged with Escherichia coli.【Method】 216 healthy 1-day-old White-feathered broilers were randomly divided into 3 groups with 6 replicates in each group and 12 birds in each replicate (6 males and 6 females).The experiment lasted for 42 d.Broilers in control group (CON group) and Escherichia coli challenge group (EC group) were fed basal diet,and the broilers in Escherichia coli challenge + puerarin group (ECPR group) were fed the basal diet supplemented with 20 mg/kg puerarin.At the age of 10 day,broilers in EC and ECPR groups were fed 1 mL Escherichia coli K88 solution (1×10⁸ CFU/mL) per chicken,while chickens in CON group were fed with the same volume of sterilized medium.On the 21st day of the experiment,blood was collected from broiler chickens.After slaughter,different intestinal segments,immune organs,and cecal chyme were collected to measure the blood biochemical indicators,immune organ indexes,blood and intestinal antioxidant indicators,and cecal microbiota of broiler chickens.【Result】 ①Puerarin had no significant effect on the growth performance and immune organ indexes of broiler challenged with Escherichia coli (P＞0.05).②Compared with CON group,the content/activity of plasma albumin (ALB),alanine aminotransferase (ALT),alkaline phosphatase (ALP),high-density lipoprotein (HDL),and uric acid (UA) in EC group were significantly increased,but the content of plasma glucose (GLU) was significantly decreased (P＜0.05).Compared with EC group,the plasma ALP activity in ECPR group was significantly decreased,while the plasma contents of calcium (Ca) and phosphorus (P) were significantly increased (P＜0.05).③Compared with CON group,plasma hydrogen peroxide (H₂O₂) content in EC group was significantly increased,whereas the duodenal glutathione peroxidase (GSH-Px) and catalase (CAT) activities,jejunal GSH-Px,superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) activities,and ileal GSH-Px activities were significantly decreased (P＜0.05).Compared with EC group,plasma malondialdehyde (MDA) and H₂O₂ contents in ECPR group were significantly decreased,but duodenal GSH-Px and CAT activities,jejunal GSH-Px and iNOS activities,and ileal GSH-Px activity in ECPR group were significantly increased (P＜0.05).④Compared with CON group,the content of Escherichia coli in the cecum in EC group was significantly increased (P＜0.05).【Conclusion】 Dietary supplemented with 20 mg/kg puerarin could effectively mitigate the adverse effects on broiler chickens challenged with Escherichia coli by regulating blood biochemical indexes and improving intestine antioxidant capacity.

Key words: puerarin; Escherichia coli; broiler; growth performance; caecum microflora

CLC Number:

S816.7

ZANG Jiajia, LI Bing, TENG Yi, YANG Yuzhu, PI Dejin, CHEN Qinyin, GUO Shuangshuang, LAI Yujiao, YI Dan. Effect of Puerarin on Growth Performance,Intestinal Antioxidant Capacity and Intestinal Flora in Broilers Challenged with Escherichia coli[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4846-4857.

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Effect of Puerarin on Growth Performance,Intestinal Antioxidant Capacity and Intestinal Flora in Broilers Challenged with Escherichia coli

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