鹅细小病毒安徽分离株NS和VP基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2018.05.007

Abstract

Abstract:

To investigate the variation status of goose parvovirus (GPV) gene in Anhui,the gene of GPV was cloned by PCR,which we named as AH gene,and we compared AH gene with GPV genes of swan,Muscovy ducks,Yan goose and so on.The results showed that Anhui GPV AH gene included the complete NS and VP genes,which were 1 884 and 2 199 bp in length,and coding two nonstructural proteins and three structural proteins,respectively.Evolution analysis revealed that AH gene and Chongqing isolate RC16,Anhui isolates Y,E and Yan-2 had a close genetic relationship.The duck parvovirus strain DS15 isolated from Anhui and the virulent B strain of Hungary,in particular the vaccine strain SYG61v,were far away from AH gene.Similarity analysis showed that the nucleotide homology between AH-NS gene and the isolate RC16 was highest (99.6%).It showed the lowest homology between AH-NS gene and the vaccine strain SYG61v,which was 94.3%.The nucleotide sequences of AH-VP and the isolate RC16 were completely consistent.The homology was 95.2% between AH-VP gene and the isolate DS15.The nucleotide sites of the VP3 gene 797,1 141,1 144,1 169 and 1 185 bp were G,A,G,C and T in GPV AH gene and virulent strains,respectively.And the Anhui GPV isolate was considered as a virulent strain with the characteristics of the severe infection.The results showed that the GPV had the higher homology and a wide host range,the different waterfowls could be infected.

Key words: goose parvovirus (GPV); nonstructural protein; structural protein; sequence analysis

CLC Number:

S852.65

DAI Yin, ZHANG Danjun, ZHAO Ruihong, HU Xiaomiao, SHEN Xuehuai, HOU Hongyan, PAN Xiaocheng, ZHOU Xueli. Cloning and Sequence Analysis of NS and VP Genes of Goose Parvovirus Strain in Anhui[J]. , 2018, 45(5): 1177-1183.

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Cloning and Sequence Analysis of NS and VP Genes of Goose Parvovirus Strain in Anhui

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