Abstract: In order to study the sterilization effect of the Chinese medicine disinfectant, prepared rhubarb, mugwort and other herbs as compound disinfectant in laboratory，and quantitative bactericidal test, on-site disinfection tests and clinical experimental study were carried out. The results showed that, Chinese herbal disinfectant against Staphylococcusaureus for 10 min, the killing rate was 99.9%, Sterilization for 30 min, the killing rate was 100%; more than 10 min to Escherichia coli，the killing rate was 100%; more than 15 min to Bacillus subtilis, the killing rate was 99.96%; the killing rate of on-site disinfection bacterial was 99.96%;placed in the 54 ℃ for 14 days, sterilization effect was essentially the same, presence of organic matter had a certain influence to the disinfection effect. The results suggested that, Chinese herbal disinfectant was stable and effective, the development of disinfectant was practicable.

Key words: Chinese medicine disinfectant; Staphylococcusaureus; Escherichia coli; Bacillus subtilis; bactericidal effect