Abstract: A high-performance liquid chromatography method was developed for determination of dicyclanil in lamb and beef. The target compound was extracted from samples by acetonitrile, then was defatted by liquid-liquid extraction (LLE) with hexane, following cleaned up by a HLB SPE column. Finally, the prepared sample was determined by HPLC/UV at 270 nm. The calibration curve showed excellent linearity in the range of 20 to 1000 μg/L with correlation coefficient of 0.99. The mean average recoveries of dicyclanil ranged from 81.8% to 93.4% at spiked levels of 100 to 1000 μg/kg, with intra-assay (n=5) and inter-assay (n=3) coefficient of variation (CV) below 11.9% and 9.4%, respectively. The limit of detection (LOD) was 30.0 μg/kg for lamb and 28.0 μg/kg for beef. The limit of quantitation (LOQ) was 100.0 μg/kg for lamb and 93.1 μg/kg for beef. The results demonstrated that the method was simple, sensitive and accurate. It is applicable to detect dicyclanil in original food.

Key words: HPLC; lamb; beef; Dicyclanil