长途运输影响安格斯育肥牛肝脏功能关键基因的筛选

doi:10.16431/j.cnki.1671-7236.2023.10.023

Abstract

Abstract: 【Objective】 This experiment was conducted to study the effects of long-distance transportation on body weight,slaughter rate and liver mRNA expression profile of fattening cattle and to screen for key genes affecting liver function in long-distance transportation.【Method】 Twenty-two Angus fattening bulls of similar weight were randomly divided into 2 groups,9 of which were slaughtered on the spot in the control group,and 13 of which were slaughtered after long-distance transportation in the experimental group,and the effect of long-distance transportation on body weight and slaughter rate was observed.In addition,three bovine liver tissues of similar weight were collected in the two groups.Transcriptome sequencing (RNA-Seq) was performed to screen differentially expressed genes,and GO functional annotation and KEGG pathway enrichment analysis were performed on them.【Result】 The live body weight of cattle decreased by 4.5% after long-distance transportation,and there was no significant difference in slaughter rate compared with the control group (P＞0.05).The RNA-Seq results showed that 182 genes were significantly upregulated and 91 genes were significantly downregulated in liver tissues of the long-distance transportation group.GO enrichment and KEGG pathway analysis showed that 182 up-regulated genes were significantly enriched in the terms of regulation of response to stimulus,peroxisome,oxidoreductase activity,cAMP signaling pathway,MAPK signaling pathway,and longevity regulating pathway.According to the results of GO enrichment and KEGG pathway analysis,four genes (ACOX3,FOS, HSPA1A,HSPA6) were screened out that were closely related to the changes of liver energy metabolism,liver cell damage and post-injury adaptive protection mechanism of fattening cattle after long-distance transportation.【Conclusion】 The live weight of Angus fattening cattle was reduced by 4.5% after long-distance transportation.Four key genes ACOX3,FOS,HSPA1A and HSPA6 affecting liver function of Angus fattening cattle after long-distance transportation were screened.The results provided a reference for future research on the mechanism,prevention and treatment of transport stress in beef cattle.

Key words: long-distance transportation; slaughter rate; liver function; beef cattle

CLC Number:

S811.2

SUN Wenyang, XIE Jianliang, SHI An, CHEN Zhilong, JIANG Wu, WANG Ruigang, HOU Pengxia, LIANG Xiaojun. Screening of Key Genes Affecting Liver Function in Angus Fattening Cattle After Long-distance Transportation[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4096-4105.

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Screening of Key Genes Affecting Liver Function in Angus Fattening Cattle After Long-distance Transportation

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