Abstract: 【Objective】 A high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-MS/MS) method was developed for the determination of pentachlorophenol (PCP) in swine feed,feed additives and water.【Method】 The samples were extracted with aqueous alkaline methanolic solution,enriched and cleaned up using an anionic solid phase extraction column,reconstituted with 2% formate methanol after nitrogen drying.With 5 mmol/L ammonium acetate solution-methanol as mobile phase,Poroshell 120 EC-C18 (3 mm×100 mm,2.7 μm) column was used to separate PCP.Under the negative ionization,multiple reaction monitoring mode (MRM) was used to determine the content of PCP.Matrix-matched external quantification method was utilized to quantitate PCP.Linear relationship,limits of detection (LOD),limits of quantification (LOQ),recovery,accuracy,precision,matrix effect and other indicators of the established method were investigated.【Result】 The LOD and LOQ were 0.1 and 0.5 μg/kg in livestock feed,feed ingredients and feed additives,while 5 and 12.5 ng/L in farm water,respectively.The linear range of PCP in livestock feed,feed ingredients and feed additives was from 0.5 to 20 μg/kg,and the linear range of farm water was from 12.5 to 500 ng/L.The linear coefficient (R²) was more than 0.999,showing the good linearity.In addition,the recoveries ranged from 69.8% to 119.5% and the relative standard deviation (RSD) were from 1.62% to 9.86%.The matrix effect (ME) was 0.62-1.29.【Conclusion】 The method established in this study with high sensitivity,high accuracy and good robustness was suitable for the screening of PCP in swine formula feed and other farming inputs to ensure the quality and safety of meat products.

Key words: pentachlorophenol; feed; solid phase extraction; HPLC-MS/MS