基于16S rDNA测序分析不同剩余采食量皖南黄兔盲肠中微生物菌落多样性

doi:10.16431/j.cnki.1671-7236.2024.02.012

Abstract

Abstract: 【Objective】 The aim of this study was to explore the relationship between the level of residual feed intake in Wannan Yellow rabbits and the diversity of cecal microbiota.【Method】 A total of 110 rabbits with similar body weight and similar days of age were selected for the feeding experiment lasting 60 days.The residual feed intake (RFI) was measured after the experiment.Ten rabbits with high (n=5) and low (n=5) RFI were selected and divided into the H (high RFI) and L (low RFI) groups.16S rDNA amplification sequencing technique was used to compare and analyze the cecal contents of H and L groups.【Result】 There was no significant difference in the abundance between these two groups (P>0.05).However, the bacterial diversity was different.The relative abundance of Fusicatenibacter, Blautia, Lachnospira, Oxalobacter and Intestinibacter in L groups was significantly higher than that in the H groups (P＜0.05), while the abundance of paludicola, Mailhella, Helicobacter, Pseudomonas was significantly higher in L groups (P＜0.05).【Conclusion】 There were some differences in the diversity and structure of cecal microflora in different RFI of Wannan Yellow rabbits, including Lachnospira, Fusicatenibacter, Colidextribacter, which were related to lipid metabolism and blood glucose regulation.Adjusting the structure and abundance of intestinal microflora may be an effective way to improve the feed efficiency of rabbits.

Key words: caecum microorganism; 16S rDNA sequencing; Wannan Yellow rabbit; residual feed intake

CLC Number:

S816.5⁺3

WANG Yuanlang, QI Pingping, DING Haisheng, ZHAO Huiling, HUANG Dongwei. Analysis of Microbial Colony Diversity in Cecum of Wannan Yellow Rabbits for Different Residual Feed Intake Based on the 16S rDNA Sequencing[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 549-557.

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Analysis of Microbial Colony Diversity in Cecum of Wannan Yellow Rabbits for Different Residual Feed Intake Based on the 16S rDNA Sequencing

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