以16S rDNA高通量测序法研究果寡糖对奶牛瘤胃菌群结构及多样性的影响

doi:10.16431/j.cnki.1671-7236.2018.10.013

Abstract

Abstract:

This experiment was aimed to study the effect of fructooligosaccharides on the structure and diversity of rumen bacterial community in dairy cows by 16S rDNA sequencing technology.Four lactating dairy cows with similar lactation stages and the same parity were randomly divided into two groups using two-phase cross design method.The cows in control group were fed with basal diet and that in test group were fed with the basal diet with 60 g/(d·head) fructooligosaccharides.The experiment was designed through 2×2 cross-over test with 21 d in each stage (14 d for pre-experiment and 7 d for test) and 14 d transitional period of cross test.The rumen fluid samples were collected at 0 (before feeding),2,4,6,9 and 12 h after feeding by cattle catheter.Each phase was collected continuously for 3 days,and the structure and diversity of rumen bacterial community were measured by 16S rDNA sequencing technology.The results of Alpha diversity indexes (Simpson,Shannon),richness indexes (Chao,Ace) and Beta diversity profile showed that the addition of fruitooligosaccharide decreased the bacterial diversity in the rumen of dairy cows.And the results of 16S rDNA sequencing showed that according to the analysis at phylum level,Bacteroidetes,Firmicutes and Proteobacteria accounted for more than 95% of the total bacterial population in cow rumen of two groups.The addition of fructooligosaccharides significantly decreased the abundance of Bacteria_NA (P < 0.05),and the abundance of Synergistetes showed an increasing trend (P=0.075).According to the analysis at genus level,fructooligosaccharides significantly increased the abundance of the Pseudomonas (P < 0.05),extremely significantly increased the abundance of Bacteroides (P < 0.01),while the abundance of Dehalobacterium significantly decreased (P < 0.05).Compared with the control group,the abundance of Ruminococcus,Butyrivibrio,Succiniclasticum and Lachnospiraceae_NA in test group was increased by 80.0%,7.5%,127.9% and 20.0%,respectively,but these differences were not significant (P > 0.05).Under the test conditions,the addition of fructooligosaccharides had a certain effect on the diversity and structure of rumen bacterial flora in dairy cows,which significantly increased the abundance of Pseudomonas which unfermented sugar,extremely significantly increased the abundance of Bacteroides which used carbohydrates as a source of fermentation,had an accelerating effect on the abundance of rumen fiber degrading bacteria.

Key words: fructooligosaccharides; Chinese Holstein dairy cows; rumen bacterial flora; 16S rDNA sequencing technology

CLC Number:

S816.7

HU Dandan, GUO Tingting, LI Na, ZHANG Lili, XU Xiaofeng. Effect of Fructooligosaccharide on the Structure and Diversity of Rumen Bacterial Community in Dairy Cow by 16S rDNA Sequencing Technology[J]. , 2018, 45(10): 2761-2771.

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Effect of Fructooligosaccharide on the Structure and Diversity of Rumen Bacterial Community in Dairy Cow by 16S rDNA Sequencing Technology

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