高精料饲粮条件下添加苦豆子生物碱对杜蒙羔羊生长性能和血清生化指标的影响

doi:10.16431/j.cnki.1671-7236.2024.11.012

Abstract

Abstract: 【Objective】 This experiment was conducted to investigate the effects of Sophora alopecuroides alkaloids on growth performance and blood indexes of Dumeng lambs fed with high-concentrate diet. 【Method】 Thirty-two Dumeng lambs with body weight of (25.76±2.35) kg were randomly divided into 4 groups with 8 lambs in each group.The basal diet of each experimental group was the same,which was a high-concentrate diet with a concentrate to forage ratio of 7∶3,and the lambs in control group were fed with the basal diet.The lambs in groupⅠ were fed with the diet added 0.2% Sophora alopecuroides,in group Ⅱ were fed with the diet supplemented with 0.121 g/kg Sophora alopecuroides alkaloids (low-dose alkali group),and in group Ⅲ were fed with the diet suplemented with 0.169 g/kg Sophora alopecuroides alkaloids (high-dose alkali group).The pre-feeding period was 15 days,and the feeding period was 60 days.The lambs were fed in a single cage and fed ad libitum.During the experiment,the daily feed intake of each sheep was recorded.The lambs were weighed with an empty stomach on days 0,30 and 60 of the experiment to calculate growth performance indicators.After weighing on the 60th day,blood was collected from the jugular vein of the lambs for measurement of serum biochemical index. 【Result】 The average daily gain of lambs in groups Ⅰ and Ⅱ were significantly higher than that in control group and group Ⅲ from 0 to 30 d (P＜0.05).From 30 to 60 d,the average daily gain and daily feed intake of lambs in group Ⅱ were significantly higher than those in group Ⅲ (P＜0.05).From 0 to 60 d,the average daily gain of lambs in group Ⅱ was significantly higher than that in group Ⅲ (P＜0.05).On the 60 th day of the experiment,compared with group Ⅲ,the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in groups Ⅰ and Ⅱ were significantly decreased (P＜0.05),and the contents of total protein (TP) and albumin (ALB) in serum of lambs in group Ⅱ were significantly increased (P＜0.05).Compared with control group,the serum glucose (GLU) contents of groups Ⅰ, Ⅱ and Ⅲ were significantly decreased (P＜0.05),the content of serum immunoglobulin M (IgM) in group Ⅱ was extremely significantly increased (P＜0.01),and the content of serum IgA in group Ⅱ was significantly higher than that in group Ⅲ (P＜0.05). 【Conclusion】 The addition of 0.121 g/kg Sophora alopecuroides alkaloids under high-concentrate diet could improve the growth performance of Dumeng lambs,alleviate liver damage,reduce GLU content and regulate humoral immune function.

Key words: Sophora alopecuroides; alkaloid; lambs; growth performance; serum biochemical indexes

CLC Number:

S826.42

ZHAO Jianxin, LI Shufang, LU Henan, LI Shaojun, GAO Aiwu, WANG Hairong. Effects of Alkaloids from Sophora alopecuroides on Growth Performance and Serum Biochemical Indexes of Dumeng Lambs Fed with High Concentrate Diet[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4774-4782.

References

[1] OETZEL G R.Diagnosis and management of subacute ruminal acidosis in dairy herds[J]. Veterinary Clinics of North America Food Animal Practice, 2017,33(3):463-480.
[2] 谢明欣.苦豆子对蒙古羔羊体外发酵参数、微生物种群及血液中生理生化指标的影响[D].呼和浩特：内蒙古农业大学,2018.
XIE M X.Effects of Sophora alopecuroides on in vitro fermentation parameters,microbial population and physiological and biochemical indexes in blood of Mongolian lambs[D].Hohhot：Inner Mongolia Agricultural University,2018.(in Chinese)
[3] 刘艳琴.苦豆子综合开发利用生产工艺的研究[D].西安：西北大学,2015.
LIU Y Q.Study on the comprehensive development and utilization of the production process of Sophora alopecuroides[D].Xi’an：Northwest University,2015.(in Chinese)
[4] 游菁菁,李月英,沙碧莹,等.中药苦豆子生物碱的研究进展[J].江西中医药大学学报,2015,27(2):109-113.
YOU J J,LI Y Y,SHA B Y,et al.Research progress of alkaloids in Sophora alopecuroides [J]. Journal of Jiangxi University of Chinese Medicine,2015,27(2):109-113.(in Chinese)
[5] WANG C,CHOI Y H,XIAN Z M,et al.Aloperine suppresses allergic airway inflammation through NF-κB,MAPK,and Nrf2/HO-1 signaling pathways in mice[J].International Immunopharmacology,2018,65:571.
[6] 范秋雨,武建文,李春晓,等.苦豆子抗炎机制的网络药理学分析及试验验证[J].中国畜牧兽医,2023,50(7):2951-2965.
FAN Q Y,WU J W,LI C X,et al.Network pharmacological analysis and experimental verification of the anti-inflammatory mechanism of Sophora alopecuroides[J].China Animal Husbandry & Veterinary Medicine,2023,50(7):2951-2965.(in Chinese)
[7] 冷晓红,王志强,李军.苦豆子生物碱对奶牛乳房炎致病菌体外抗菌活性的研究[J].中国兽药杂志,2013,47(9):31-33.
LENG X H,WANG Z Q,LI J.Study on in vitro antibacterial activity of Sophora alopecuroides alkaloids against pathogenic bacteria of dairy cow mastitis [J].Chinese Journal of Veterinary Drug,2013,47(9):31-33.(in Chinese)
[8] 刘静,杨树鑫.苦豆子生物碱抗肿瘤作用的分子机制[J].海峡药学,2015,27(6):43-46.
LIU J,YANG S X.Molecular mechanism of anti-tumor effect of Sophora alopecuroides alkaloids [J].Strait Pharmaceutical Journal,2015,27(6):43-46.(in Chinese)
[9] 朱金华,万红娇,熊秋迎,等.槐定碱对内毒素致小鼠肺损伤的抗氧化作用及对NF-κB表达的影响[J].中国实验方剂学杂志,2011,17(20):182-186.
ZHU J H,WAN H J,XIONG Q Y,et al.Antioxidant effect of sophoridine on endotoxin-induced lung injury in mice and its effect on NF-κB expression [J]. Chinese Journal of Experimental Traditional Medicine Formulae,2011,17(20):182-186.(in Chinese)
[10] 王一帆,范恒.氧化苦参碱调控RhoA/ROCK信号通路介导溃疡性结肠炎E-cadherin及TGF-β的影响[J].中国实验方剂学杂志,2019,25(6):73-80.
WANG Y F,FAN H.The effect of oxymatrine on E-cadherin and TGF-β in ulcerative colitis mediated by RhoA/ROCK signaling pathway [J].Chinese Journal of Experimental Traditional Medicine Formulae,2019,25(6):73-80.(in Chinese)
[11] 李述方,马甜,杨金丽,等.高精料饲粮条件下苦豆子对育肥羔羊瘤胃上皮c-Jun氨基末端蛋白激酶/p38丝裂原活化蛋白激酶信号通路及紧密连接蛋白表达的影响[J].动物营养学报,2022,34(6):3940-3952.
LI S F,MA T,YANG J L,et al.Effects of Sophora alopecuroides on c-Jun N-terminal protein kinase/p38 mitogen-activated protein kinase signaling pathway and tight junction protein expression in rumen epithelium of fattening lambs under high-concentrate diet [J].Chinese Journal of Animal Nutrition,2022,34(6):3940-3952.(in Chinese)
[12] 安雅雯,杨晓东,高智雄,等.高精饲粮中添加苦豆子对羔羊生长与血液生化指标的影响[J].浙江农业学报,2022,34(5):908-914.
AN Y W,YANG X D,GAO Z X,et al.Effects of adding Sophora alopecuroides to high-precision diet on growth and blood biochemical indexes of lambs[J].Acta Agricultural Zhejiangensis,2022,34(5):908-914.(in Chinese)
[13] 杨文辉.中草药饲料添加剂在养殖业中的应用研究[J].中兽医学杂志,2018,1:74-75.
YANG W H.Application of Chinese herbal feed additives in animal husbandry[J].Chinese Journal of Traditional Veterinary Science,2018,1:74-75.(in Chinese)
[14] WANG M，SUN J，JIANG Z，et al.Hepatoprotective effect of kaempferol against alcoholic liver injury in mice[J].The American Journal of Chinese Medicine，2015,43(2):241-254.
[15] ZHOU Y，TAN F，LI C，et al.White peony (fermented Camellia sinensis) polyphenols help prevent alcoholic liver injury via antioxidation[J]. Antioxidants (Basel,Switzerland) ,2019,8(11):524.
[16] 郭峻菲.亚急性瘤胃酸中毒(SARA)对反刍动物肝脏的炎性损伤与SARA的调控研究[D].南京：南京农业大学,2019.
GUO J F.Subacute rumen acidosis (SARA) on inflammatory injury of ruminant liver and regulation of SARA[D].Nanjing:Nanjing Agricultural University,2019.(in Chinese)
[17] 苏永霞.苦豆子种子成分分析与毒性评价及生物碱抗小鼠结肠炎的作用研究[D].杨凌：西北农林科技大学,2022.
SU Y X.Study on the composition analysis and toxicity evaluation of Sophora alopecuroides seeds and the effect of alkaloids on colitis in mice[D].Yangling:Northwest A&F University,2022.(in Chinese)
[18] 徐爱萍,陈新梅,郭辉，等.氧化苦参碱磷脂复合物对CCl₄致大鼠慢性肝损伤的影响[J].山东中医药大学学报,2020,44(1):73-78.
XU A P,CHEN X M,GUO H,et al.Effect of oxymatrine phospholipid complex on CCl₄-induced chronic liver injury in rats[J].Journal of Shandong University of Traditional Chinese Medicine,2020,44(1):73-78.(in Chinese)
[19] 郭东清,王雪飞,孙晓磊,等.苦豆籽粕-两岐双歧杆菌-唾液乳杆菌合生元对早期断奶仔猪血清生化指标及免疫球蛋白含量的影响[J].动物医学进展,2014,35(10):38-42.
GUO D Q,WANG X F,SUN X L,et al.Effects of bitter bean seed meal-Bifidobacterium bifidum-Lactobacillus salivarius synbiotics on serum biochemical indexes and immunoglobulin content in early weaned piglets[J].Progress in Veterinary Medicine ,2014,35(10)：38-42.(in Chinese)
[20] 吴旋,曾申明.血清白蛋白的功能、应用及纯化工艺研究进展[J].中国畜牧兽医,2023,50(6):2265-2275.
WU X,ZENG S M.Research progress on the function,application and purification process of serum albumin[J].China Animal Husbandry & Veterinary Medicine,2023,50(6):2265-2275.(in Chinese)
[21] 陈立波.注射用盐酸苦参碱对化学性及免疫性肝损伤的影响[D].沈阳：沈阳药科大学,2007.
CHEN L B.Effect of matrine hydrochloride for injection on chemical and immunological liver injury[D].Shenyang:Shenyang Pharmaceutical University,2007.(in Chinese)
[22] 张育浩,张得钧,张本印.具有降血糖活性的生物碱及其作用机制[J].中草药,2018,49(15):3692-3702.
ZHANG Y H,ZHANG D J,ZHANG B Y.Alkaloids with hypoglycemic activity and their mechanism of action[J].Chinese Traditional and Herbal Drugs,2018,49(15):3692-3702.(in Chinese)
[23] 王德珍.黄连生物碱降糖作用及构效关系初探[D].重庆：西南大学,2014.
WANG D Z.Preliminary study on hypoglycemic effect and structure-activity relationship of coptis alkaloids[D].Chongqing: Southwest University,2014.(in Chinese)
[24] 李昊.日粮中添加苦豆子对滩羊体外发酵参数、营养物质消化、生长及肉品质影响的研究[D].银川：宁夏大学,2020.
LI H.Effects of dietary addition of Sophora alopecuroides on in vitro fermentation parameters,nutrient digestion,growth and meat quality of Tan sheep[D].Yinchuan:Ningxia University,2020.(in Chinese)
[25] CHEN D, CHEN X,WANG B,et al.Effects of mulberry leaf flavonoid and resveratrol on methane emission and nutrient digestion in sheep[J].Animal Nutrition,2015,1(4):362-367.
[26] 董晓丽,张乃锋,周盟,等.复合菌制剂对断奶仔猪生长性能、粪便微生物和血清指标的影响[J].动物营养学报,2013,25(6):1285-1292.
DONG X L,ZHANG N F,ZHOU M,et al.Effects of compound bacteria preparation on growth performance,fecal microorganisms and serum indexes of weaned piglets[J].Chinese Journal of Animal Nutrition,2013,25(6):1285-1292.(in Chinese)
[27] YAN F,POLK D B.Probiotics and immune health[J].Current Opinion in Gastroenterology,2011,27(6):496-501.
[28] RAHMAN T,GIER C D,ORAMI T,et al.PCV10 elicits protein D IgG responses in Papua New Guinean children but has no impact on NTHi carriage in the first two years of life[J].Vaccine,2021,39(26):3486-3492.
[29] SUN P,WANG J Q,ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of preweaning calves[J].Journal of Dairy Science,2010,12:93.
[30] TSAI Y T,CHENG P C,PAN T M,et al.The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits[J].Applied Microbiology and Biotechnology,2012,96:853-862.
[31] WEII S J M.Immunomodulatory mechanisms of lactobacilli[J].Microbial Cell Factories,2011,10(Suppl 1):S17.
[32] 郭东清.合生元对早期断奶仔猪血液指标、肠黏膜免疫及肠道菌群影响的研究[D].呼和浩特：内蒙古农业大学,2014.
GUO D Q.Effects of synbiotics on blood parameters,intestinal mucosal immunity and intestinal flora of early weaned piglets[D].Hohhot:Inner Mongolia Agricultural University,2014.(in Chinese)
[33] 安雅雯,杨晓东,郭绍乾,等.高精饲粮中添加苦豆子对杜蒙羔羊血清免疫及抗氧化指标的影响[J].云南农业大学学报(自然科学),2022,37(5):804-811.
AN Y W,YANG X D,GUO S Q,et al.Effects of adding Sophora alopecuroides in high-precision diet on serum immune and antioxidant indexes of Dumeng lambs[J].Journal of Yunnan Agricultural University (Natural Science),2022,37(5):804-811.(in Chinese)

Effects of Alkaloids from Sophora alopecuroides on Growth Performance and Serum Biochemical Indexes of Dumeng Lambs Fed with High Concentrate Diet

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