To provide a scientific basis for the preparation of dairy diet, the dried distillers grains with soluble (DDGS) samples collected were used to evaluate the ruminal degradation rate in cow. Four ruminally cannulated Chinese Holstein dairy cows in dry period were used to determine the ruminal degradation rate of DDGS of various sources with nylon bag technique. Results showed that dry matter (DM) was about 90% for most of the DDGS samples, crude protein (CP) differed from different plants (26.4% to 32.5% CP).Ruminal degradation parameter of DM of DDGS differed between different pants. The water-soluble fraction of DM was greatest for HB2 and HLJ2, lowest for HN and TJ and mid for other samples. The degradable fraction of DM was lower for HLJ1, HLJ2, HB1 and HB2 with about 60%, and higher for TJ, AH and HN with about 75%.〖HT〗〖ST〗〖WT〗〖HJ〗

〖WT5”BZ〗The degradation rate of degradable fraction ranged from 0.025% to 0.044 h-1.Effective degradation rate of DM ranged from 41.0% to 58.2%. Water-soluble fraction of CP was about 17%, except TJ with 3.3%. Degradable fraction of CP was the least for HB1 and HLJ1 with 58.7%, and greatest for HB2 and HLJ2 with 82.2% and 82.1%. The degradation rate of degradable fraction of CP ranged from 0.013 to 0.035 h-1. The effective degradation rate of CP ranged from 22.5% to 53.4%. In concern with the nutritional characteristic and ruminal degradation parameter of DDGS differed largely in various plants, it would be effective to develop a quick and easy test technique to determine the feed value of the DDGS that could be used by the farmer. Then a more efficient use of nitrogen was to reduce wastage of feed resources.

The aim of the present study was to explore the genetic polymorphisms of myogenin (MyoG)gene in Cervidae. With the blood of 56 Gansu wapiti as experimental materials, PCR-SSCP and DNA sequencing approaches were applied to detect the single nucleotide polymorphisms (SNPs) and analyze the sequence variation at partial 5′UTR of MyoG gene. The results indicated that: ① The products amplified by primer 1 displayed polymorphism, three genotypes(AA, AB and BB) were detected in Gansu wapiti. The sequencing results showed that there was one single nucleotide mutation (G-237→A-237) at 5′UTR of MyoG gene. The polymorphism site was controlled by two alleles of A and B. Analysis of population genetics suggested that 〖WT〗〖ST〗〖HT〗〖HJ〗

〖WT5”BZ〗the genotype frequency of AA was the highest and A was the dominant allele gene. They were in low polymorphism (PIC=0.194) in PIC mutation site in 56 Gansu wapiti. There would be one Sp1 transcription factor missing at the mutation site by applying an analysis tool of TFSEARCH 1.3. ② Three genotypes (CC, CD and DD) determined by a SNP at -46 bp (G→A) were identified using primer 2, the genotype frequencies were in the order of CC＞CD＞DD, the PIC value (0.266) was intermediate polymorpgism (0.25＜PIC＜0.50). However, the potential elements and protein factors were not changed. This work would lay the foundation for further study on relationship between SNPs and carcass and meat quality traits in Gansu wapiti.

According to the heart fatty acid-binding protein (H-FABP) gene sequence of yak from GenBank, 13 pairs of primers were designed to amplify the whole gene sequence. The H-FABP gene of BLACK cattle was amplified, sequenced and analyzed. The nucleotide sequence was detected and the amino acid sequence of this gene was deduced. Then we compared to deduce amino acid sequence with the homologous H-FABP gene of other species. A phylogenetic tree of the H-FABP gene of BLACK cattle and other species was constructed with MEGA 4.0. The results showed that H-FABP gene of BLACK cattle(FJ756345) had 4 exons (73, 173, 102 and 54 bp) and 3 introns (3463, 1892 and 1494 bp). After comparison, the identities of the nucleotide sequence of BLACK cattle H-FABP gene with that of yak, general cattle, goat, pig, horse, human, rat, mouse and chicken were 99.3%, 99.0%, 96.3%, 92.5%, 89.8%, 88.3%, 83.3%, 82.8%, 75.6%, respectively, and the identities of the amino acid sequence of BLACK cattle H-FABP gene with that of yak, general cattle, goat, pig, horse, hu-man, rat, mouse and chicken were 99.2%, 99.2%, 96.2%, 93.2%, 91.7%, 88.7%, 86.5%, 86.5%, 77.4%, respectively The phylogenetic results showed that these species were classified into 2 branches as a whole, in which the chicken were on one branch and BLACK cattle, yak, general cattle, goat, pig, horse, human, rat and mouse were another branch. The H-FABP gene of BLACK cattle was highly conserved, the phylogenetic tree of the H-FABP gene accords with the order of evolution.

To Jinghai yellow chicken hens as materialusing PCR-SSCP technique to study IGFBP-3 gene exon 1 and partial sequence of intron 1 polymorphic site and calculate the genotype, gene frequency, chi-square value and some indicators of genetic polymorphism. The results of exon 1 was not detected polymorphic site, in intron 1 of the partial sequence detected in more than one polymorphic site, the locus polymorphism as a moderate, in the first 160 bp took place T to G mutation, leading to BB and AB type 56 days of age, body weight was significantly greater than AA type (P<0.05), AA type egg in November was significantly greater than the number of BB and AB type (P<0.05). From this initial inference of intron 1 on the egg-laying and growth was likely to have a certain role in promoting, A gene for the advantage of egg-laying, B gene for the advantage of weight.