China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (9): 52-56.

• 生物技术 • Previous Articles     Next Articles

Molecular Cloning and Characteristics of cDNA Encoding Porcine nectin-2 for PRV Receptor

DU Wei-guo1, GENG Yu-jing2, SU Hui-long1, HU Jing-jing1, LIU Yu1, ZHANG Gui-hong1   

  1. (1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2.College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: ZHANG Gui-hong

Abstract: In order to understand the structure and function of porcine nectin-2 gene, we cloned and sequenced the cDNA encoding porcine nectin-2 from the brain of pig. The 1440 bp cDNA of porcine nectin-2 encoded a polypeptide of 479 amino acids consisting of a 32-residue putative signal peptide, a 330-residue ectodomain, a 23-residue transmembrane domain, and a 94-residue cytoplasmic domain. The ectodomain contained 2 potential N-linked glycosylation sites and 6 cysteine residues. The nucleotide sequence similarity of nectin-2 between pig and dog, horse, house mouse, human, rhesus monkey, cattle, Chimpanzee were 85.4%, 85.7%, 78.6%, 82.1%, 82.1%, 81.9%, 82.1%, and the amino acid sequence similarity were 84.5%, 83.0%, 74.7%, 75.7%, 76.4%, 78.4%, 75.5% respectively. This study will lay a foundation for understanding the interactions of PRV with host.

Key words: PRV; receptor; porcine nectin-2 gene; molecular characteristics

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