猪繁殖与呼吸综合征病毒流行株Nsp2基因的克隆及序列分析

›› 2010, Vol. 37 ›› Issue (9): 48-51.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of Nsp2 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains

YAN Dong-ming¹，LU Shi-ying¹，REN Hong-lin¹，ZHOU Yu¹， GONG Bin-bin¹，ZHENG Xin²，DU Yun-sheng³，WANG Guang-ming¹，LIU Zeng-shan¹

(1.Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;　2.College of Animal Science and Technology, Jinlin Agricultural University, Changchun 130118, China;　3.Kangfa Institute of Animal Drug, Changchun 130062, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: LIU Zeng-shan；WANG Guang-ming

Abstract

Abstract: The nonstructural protein 2(Nsp2) genes of porcine reproductive and respiratory syndrome virus (PRRSV) were cloned using RT-PCR from porcine tissue samples in some pig farms in Jilin province. The sequences of Nsp2 were sequenced and analyzed using DNAStar 7.0,Clustalx 1.83 and MEGA 4. Homology searches were performed by means of BLAST programs by NCBI. Identities of nuclear acid sequences and amino acid sequences deduced from Nsp2 genes cloned were 62.0% to 87.5% and 34.4% to 59.0% with PRRSV standard strains VR-2332 and CH-1a and so on, were 97.8% to 98.9% and 95.1% to 98.4% with Chinese highly pathogenic PRRSV strains JXA1 and HUB2 and so on.Therefore,PRRSVs leading to the infectious disease in some pig farms in Jilin province were high homology with highly pathogenic PRRSV strains.

Key words: porcine reproductive and respiratory syndrome virus; nonstructural protein 2 gene; sequence analysis

CLC Number:

S852.65⁺9.6

YAN Dong-ming;LU Shi-ying;REN Hong-lin;ZHOU Yu;GONG Bin-bin;ZHENG Xin;DU Yun-sheng;WANG Guang-ming;LIU Zeng-shan. Cloning and Sequence Analysis of Nsp2 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains[J]. , 2010, 37(9): 48-51.

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Cloning and Sequence Analysis of Nsp2 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains

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