猪源病毒细胞毒性T淋巴细胞表位研究进展

doi:10.16431/j.cnki.1671-7236.2024.07.033

Abstract

Abstract: The major histocompatibility complex (MHC) class Ⅰ molecules,also known as swine leukocyte antigen (SLA) class Ⅰ molecules,play an important role in antigen presentation,organ transplantation,immune response and regulation.Cytotoxic T lymphocyte (CTL) epitopes are linear peptides that bind and present SLA-Ⅰ molecules after antigen processing by antigen-presenting cells.With high specificity,CTL epitopes play a key role in antigen recognition and presentation and cellular immunity against viral infection.CTL epitopes can stimulate CTLs to play the role of cell killing,which is mainly manifested as killing virus.The author reviewed the current status of CTL epitopes of several important porcine viruses,such as Foot-and-mouth disease virus,African swine fever virus and Porcine reproductive and respiratory syndrome virus,and analyzed the binding motif characteristics of SLA-Ⅰ and CTL epitopes by bioinformatics,in order to provide a reference for studying CTL epitopes derived from porcine viruses and designing the epitope vaccine in future.

Key words: cytotoxic T lymphocyte (CTL) epitopes; Porcine foot-and-mouth disease virus; African swine fever virus; Porcine reproductive and respiratory syndrome virus

CLC Number:

S852.4

XIAN Yuhan, FENG Hongsheng, GAO Yongyu, LI Haiyang, YANG Siyu, SANG Chenjun, CAO Yudie, TANG Yue, LI Zibin, GAO Fengshan. Advances in Cytotoxic T Lymphocyte Epitopes of Porcine Viruses[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3086-3099.

References

[1] KNIGHT-JONES T J D,RUSHTON J.The economic impacts of foot and mouth disease-What are they,how big are they and where do they occur?[J].Preventive Veterinary Medicine,2013,112(3-4):161-173.
[2] 绪欣.口蹄疫的特点以及规模化猪场生物安全体系构建[J].中国动物保健,2023,25(2):91-92. XU X.Characteristics of foot-and-mouth disease and the construction of biosecurity system in large-scale pig farms[J].China Animal Health,2023,25(2):91-92.(in Chinese)
[3] NEUMANN E J,KLIEBENSTEIN J B,JOHNSON C D,et al.Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States[J]. Journal of the American Veterinary Medical Association,2005,227(3):385-392.
[4] ZHENG X J,NIE S M,FENG W H.Regulation of antiviral immune response by African swine fever virus (ASFV)[J].Virologica Sinica,2022,37(2):157-167.
[5] 郭良珍,苗国防.非洲猪瘟的防控技术[J].北方牧业,2023,4:33. GUO L Z,MIAO G F.Prevention and control techniques for African swine fever[J].Beifang Muye,2023,4:33.(in Chinese)
[6] YANG D,SUN C,GAO R,et al.A temperature-dependent translation defect caused by internal ribosome entry site mutation attenuates Foot-and-mouth disease virus:Implications for rational vaccine design[J]. Journal of Virology,2020,94(16):e00990-20.
[7] ZHANG H,LUO Q,HE Y,et al.Research progress on the development of porcine reproductive and respiratory syndrome vaccines[J]. Veterinary Sciences,2023,10(8):491.
[8] SEREDA A D,KAZAKOVA A S,NAMSRAYN S G,et al.Subsequent immunization of pigs with African swine fever virus (ASFV) seroimmunotype Ⅳ vaccine strain FK-32/135 and by recombinant plasmid DNA containing the CD2v derived from MK-200 ASFV seroimmunotype Ⅲ strain does not protect from challenge with ASFV seroimmunotype Ⅲ[J].Vaccines (Basel),2023,11(5):1007.
[9] STENFELDT C,ARZT J.The carrier conundrum;A review of recent advances and persistent gaps regarding the carrier state of Foot-and-mouth disease virus[J].Pathogens,2020,9(3):167.
[10] DE LOS SANTOS T,DIAZ-SAN SEGUNDO F,RODRIGUEZ L L.The need for improved vaccines against foot-and-mouth disease[J].Current Opinion in Virology,2018,29:16-25.
[11] BERGMANN I E,MALIRAT V,PEDEMONTE A,et al.Challenges in Foot-and-mouth disease virus strain selection as an input to attain broad vaccine intraserotype cross-protection[J].Expert Review of Vaccines,2021,20(1):13-22.
[12] O'DONNELL V,HOLINKA L G,SANFORD B,et al.African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge[J].Virus Research,2016,221:8-14.
[13] DIAZ-SAN SEGUNDO F,SALGUERO F J,DE AVILA A,et al.Selective lymphocyte depletion during the early stage of the immune response to Foot-and-mouth disease virus infection in swine[J].Journal of Virology,2006,80(5):2369-2379.
[14] CAI L,ZHANG J,ZHU R,et al.Protective cellular immunity generated by cross-presenting recombinant overlapping peptide proteins[J].Oncotarget,2017,8(44):76516-76524.
[15] SECOMBES C J,BIRD S,ZOU J.Adaptive immunity in teleosts:Cellular immunity[J].Developments in Biologicals (Basel),2005,121:25-32.
[16] DONG S,WANG P,ZHAO P,et al.Direct loading of iTEP-delivered CTL epitope onto MHC class Ⅰ complexes on the dendritic cell surface[J].Molecular Pharmaceutics,2017,14(10):3312-3321.
[17] KHANNA R,SILINS S L,WENG Z P,et al.Cytotoxic T cell recognition of allelic variants of HLA B35 bound to an Epstein-Barr virus epitope:Influence of peptide conformation and TCR-peptide interaction[J].European Journal of Immunology,1999,29(5):1587-1597.
[18] SZETO C,LOBOS C A,NGUYEN A T,et al.TCR recognition of peptide-MHC-Ⅰ:Rule makers and breakers[J].International Journal of Molecular Sciences,2020,22(1):68.
[19] MACNABB B W,KLINE J.MHC cross-dressing in antigen presentation[J].Advances in Immunology,2023,159:115-147.
[20] RANGA V,NIEMELA E,TAMIRAT M Z,et al.Immunogenic SARS-CoV-2 epitopes:In silico study towards better understanding of COVID-19 disease-paving the way for vaccine development[J].Vaccines (Basel),2020,8(3):408.
[21] RAND L M,WOODWARD C,MAY R,et al.Divergence between genes but limited allelic polymorphism in two MHC class Ⅱ A genes in Leach's storm-petrels Oceanodroma leucorhoa[J].Immunogenetics,2019,71(8-9):561-573.
[22] ABBAS AK,LICHTMAN A H,PILLAI S.Cellular and Molecular Immunology[M].Holland:Elsevier,2018.
[23] SVITEK N,SAYA R,ZHANG H,et al.Systematic determination of TCR-antigen and peptide-MHC binding kinetics among field variants of a theileria parva polymorphic CTL epitope[J].Journal of Immunology,2022,208(3):549-561.
[24] ZHANG L,LI Z,TANG Z,et al.Efficient identification of Tembusu virus CTL epitopes in inbred HBW/B4 ducks using a novel MHC class Ⅰ-restricted epitope screening scheme[J].Journal of Immunology,2022,209(1):145-156.
[25] MACDONALD I K,HARKIOLAKI M,HUNT L,et al.MHC class Ⅰ bound to an immunodominant Theileria parva epitope demonstrates unconventional presentation to T cell receptors[J].PLoS Pathogens,2010,6(10):e1001149.
[26] WIECZOREK M,ABUALROUS E T,STICHT J,et al.Major histocompatibility complex (MHC) class Ⅰ and MHC class Ⅱ proteins:Conformational plasticity in antigen presentation[J].Frontiers in Immunology,2017,8:292.
[27] DIXON A M,ROY S.Role of membrane environment and membrane-spanning protein regions in assembly and function of the class Ⅱ major histocompatibility complex[J].Human Immunology,2019,80(1):5-14.
[28] DEL VAL M,ANTON L C,RAMOS M,et al.Endogenous TAP-independent MHC-Ⅰ antigen presentation:Not just the ER lumen[J].Current Opinion in Immunology,2020,64:9-14.
[29] KOYANAGI N,KAWAGUCHI Y.Evasion of the cell-mediated immune response by Alphaherpesviruses[J].Viruses,2020,12(12):1354.
[30] NEEFJES J,JONGSMA M L,PAUL P,et al.Towards a systems understanding of MHC class Ⅰ and MHC class Ⅱ antigen presentation[J].Nature Reviews Immunology,2011,11(12):823-836.
[31] ZHOU X,JIA X,HUANG Z,et al.MHC class Ⅱ regulation of CD8⁺ T cell tolerance and implications in autoimmunity and cancer immunotherapy[J].Cell Reports,2023,42(11):113452.
[32] GRUBMAN M J,BAXT B.Foot-and-mouth disease[J].Clinical Microbiology Reviews,2004,17(2):465-493.
[33] MATEU M G,CAMARERO J A,GIRALT E,et al.Direct evaluation of the immunodominance of a major antigenic site of Foot-and-mouth-disease virus in a natural host[J].Virology,1995,206(1):298-306.
[34] FERNANDEZ-SAINZ I,GAVITT T D,KOSTER M,et al.The VP1 G-H loop hypervariable epitope contributes to protective immunity against Foot and mouth disease virus in swine[J].Vaccine,2019,37(26):3435-3442.
[35] MARTINEZ M A,HERNANDEZ J,PICCONE M E,et al.Two mechanisms of antigenic diversification of Foot-and-mouth disease virus[J].Virology,1991,184(2):695-706.
[36] 李阳,马园,王国华,等.口蹄疫病毒O型泛亚株VP1蛋白的二级结构及抗原表位预测[J].中国动物检疫,2021,38(11):101-109. LI Y,MA Y,WANG G H,et al.Prediction of the secondary structure and epitopes of VP1 protein of FMDV-O PanAsia strain[J].China Animal Health Inspection,2021,38(11):101-109.(in Chinese)
[37] 尚延丽,冯霞,靳野,等.口蹄疫病毒A/GDMM/CHA/2013株结构蛋白VP1 H-2d限制性CTL表位预测[J].安徽农业科学,2015,43(16):146-149. SHANG Y L,FENG X,JIN Y,et al.Prediction of H-2d restricted CTL epitope of structural protein VP1 of FMDV A/GDMM/CHA/2013 strains[J].Journal of Anhui Agriculture Science,2015,43(16):146-149.(in Chinese)
[38] 巴利民,王振豹,齐鹏,等.猪SLA-1体外表达及其结合多肽的筛选[J].中国兽医杂志,2020,56(12):32-35. BA L M,WANG Z B,QI P,et al.Expression of swine SLA-1in vitro and screening the peptides[J].Chinese Journal of Veterinary Medicine,2020,56(12):32-35.(in Chinese)
[39] 巴利民,王振豹,齐鹏,等.猪SLA-1分子结合多肽特性分析及功能验证[J].中国兽医杂志,2020,56(10):46-48. BA L M,WANG Z B,QI P,et al.Characterization and functional verification of porcine SLA-1 molecular binding peptides[J].Chinese Journal of Veterinary Medicine,2020,56(10):46-48.(in Chinese)
[40] FENG L,GAO Y Y,SUN M,et al.The parallel presentation of two functional CTL epitopes derived from the O and Asia 1 serotypes of Foot-and-mouth disease virus and swine SLA-2*HB01:Implications for universal vaccine development[J].Cells,2022,11(24):4017.
[41] YUE C,XIANG W,HUANG X,et al.Mooring stone-like Arg (114) pulls diverse bulged peptides:First insight into African swine fever virus-derived T cell epitopes presented by swine major histocompatibility complex class Ⅰ[J].Journal of Virology,2022,96(4):e0137821.
[42] GAO F S,ZHAI X X,JIANG P,et al.Identification of two novel Foot-and-mouth disease virus cytotoxic T lymphocyte epitopes that can bind six SLA-Ⅰ proteins[J].Gene,2018,653:91-101.
[43] 李玲,夏应菊,宋新宇,等.非洲猪瘟病毒细胞嗜性的研究进展[J].中国兽医科学,2023,53(4):514-519. LI L,XIA Y J,SONG X Y,et al.Research progress on cytotoxicity of African swine fever virus[J].Chinese Veterinary Science,2023,53(4):514-519.(in Chinese)
[44] ANDRES G,ALEJO A,SALAS J,et al.African swine fever virus polyproteins pp220 and pp62 assemble into the core shell[J].Journal of Virology,2002,76(24):12473-12482.
[45] KAY-JACKSON P C,GOATLEY L C,COX L,et al.The CD2v protein of African swine fever virus interacts with the actin-binding adaptor protein SH3P7[J].Journal of General Virology,2004,85:119-130.
[46] MALOGOLOVKIN A,BURMAKINA G,TULMAN E R,et al.African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity[J].Journal of General Virology,2015,96:866-873.
[47] ROS-LUCAS A,CORREA-FIZ F,BOSCH-CAMOS L,et al.Computational analysis of African swine fever virus protein space for the design of an epitope-based vaccine ensemble[J].Pathogens,2020,9(12):1078.
[48] 王泽平,王飞飞,张芮铭,等.苏紫猪SLA-1基因多态性及其抗病潜能分析[J].中国畜牧兽医,2023,50(5):1918-1927. WANG Z P,WANG F F,ZHANG R M,et al.Analysis on polymorphism of SLA-1 gene and its disease resistance potential in Suzi pig[J].China Animal Husbandry&Veterinary Medicine,2023,50(5):1918-1927.(in Chinese)
[49] 蔡建秋,曹雪莲,杨旸,等.非洲猪瘟病毒Pig/HLJ/2018分离株MGF多基因家族优势B、T细胞抗原表位分析[J].现代畜牧科技,2021,8:1-5. CAI J Q,CAO X L,YANG Y,et al.Analysis of dominant B and T cell antigenic epitopes of the MGF multigene family of African swine fever virus Pig/HLJ/2018 isolates[J].Modern Animal Husbandry Science&Technology,2021,8:1-5.(in Chinese)
[50] 高瞻,邵军军,常艳燕,等.非洲猪瘟病毒p72蛋白抗原表位预测分析及多表位疫苗的构建[J].中国兽医杂志,2020,56(1):13-17. GAO Z,SHAO J J,CHANG Y Y,et al.Epitope prediction analysis of African swine fever virus p72 protein and construction of multi-epitope vaccine[J].Chinese Journal of Veterinary Medicine,2020,56(1):13-17.(in Chinese)
[51] HERRERA L R M,BISA E P.In silico analysis of highly conserved cytotoxic T-cell epitopes in the structural proteins of African swine fever virus[J].Veterinary World,2021,14(10):2625-2633.
[52] ZAJAC M D,SANGEWAR N,LOKHANDWALA S,et al.Adenovirus-vectored African swine fever virus pp220 induces robust antibody,IFN-γ,and CTL responses in pigs[J].Frontiers in Veterinary Science,2022,9:921481.
[53] BOSCH-CAMÓS L,LOPEZ E,NAVAS M J,et al.Identification of promiscuous African swine fever virus T-cell determinants using a multiple technical approach[J].Vaccines,2021,9(1):29.
[54] LUNNEY J K,FANG Y,LADINIG A,et al.Porcine reproductive and respiratory syndrome virus (PRRSV):Pathogenesis and interaction with the immune system[J].Annual Review of Animal Biosciences, 2016,4:129-154.
[55] VAN BREEDAM W,DELPUTTE P L,VAN GORP H,et al.Porcine reproductive and respiratory syndrome virus entry into the porcine macrophage[J].Journal of General Virology,2010,91:1659-1667.
[56] YE N,WANG B,FENG W,et al.PRRS virus receptors and an alternative pathway for viral invasion[J].Virus Research,2022,320:198885.
[57] XIE C Z,HA Z,SUN W C,et al.Construction and immunological evaluation of recombinant Adenovirus vaccines co-expressing GP3 and GP5 of EU-type Porcine reproductive and respiratory syndrome virus in pigs[J].Journal of Veterinary Medical Science,2019,81(12):1879-1886.
[58] TANG T,WANG C,PU Q,et al.Vaccination of mice with Listeria ivanovii expressing the truncated M protein of Porcine reproductive and respiratory syndrome virus induces both antigen-specific CD4⁺ and CD8⁺ T cell-mediated immunity[J]. Journal of Molecular Microbiology and Biotechnology,2019,29(1-6):74-82.
[59] LIANG C,XIA Q,ZHOU J,et al.Identification of potential SLA-Ⅰ-restricted CTL epitopes within the M protein of Porcine reproductive and respiratory syndrome virus[J]. Veterinary Microbiology,2021,259:109131.
[60] 康溥,赵梦坡,黄育浩,等.猪繁殖与呼吸综合征病毒GP5蛋白的结构与功能研究进展[J].广东农业科学,2023,50(2):104-114. KANG P,ZHAO M P,HUANG Y H,et al.Progress in structure and function of GP5 protein of Porcine reproductive and respiratory syndrome virus[J].Guangdong Agricultural Sciences,2023,50(2):104-114.(in Chinese)
[61] ZHANG M,HAN X,OSTERRIEDER K,et al.Palmitoylation of the envelope membrane proteins GP5 and M of Porcine reproductive and respiratory syndrome virus is essential for virus growth[J].PLoS Pathogens,2021,17(4):e1009554.
[62] 李奇润,魏孝辉,夏春.大白猪和长白猪新复等位基因及其多态性与结合肽谱分析[J].中国兽医杂志,2021,57(3):1-5. LI Q R,WEI X H,XIA C.Analysis of SLA-Ⅰ new alleles polymorphism and binding polypeptide spectrum in Large White and Landrace pig[J].Chinese Journal of Veterinary Medicine,2021,57(3):1-5.(in Chinese)
[63] 刘祥,陈琛,陈春琳,等.猪重要感染病毒蛋白的二级结构、抗原表位分析及三联表位多肽疫苗的重组预测[J].生物学杂志,2017,34(3):18-23. LIU X,CHEN C,CHEN C L,et al.The secondary structure,antigen epitope analysis and recombination prediction triple epitope peptide vaccine of porcine important infection virus protein[J].Journal of Biology,2017,34(3):18-23.(in Chinese)
[64] 李红.猪繁殖与呼吸综合征病毒GP5蛋白与M蛋白抗原表位筛选及应用[D].郑州:郑州大学,2021. LI H.Epitope screening and application of GP5 protein and M proteinof Porcine reproductive and respiratory syndrome virus[D].Zhengzhou:Zhengzhou University,2021.(in Chinese)
[65] PAN X,ZHANG N,WEI X,et al.Illumination of PRRSV cytotoxic T lymphocyte epitopes by the three-dimensional structure and peptidome of swine lymphocyte antigen class Ⅰ(SLA-Ⅰ)[J]. Frontiers in Immunology,2019,10:2995.
[66] LIANG C,XIA Q H,ZHOU J M,et al.Identification of potential SLA-Ⅰ-restricted CTL epitopes within the M protein of Porcine reproductive and respiratory syndrome virus[J].Veterinary Microbiology,2021,259:109131.
[67] GAO C,HE X,QUAN J,et al.Specificity characterization of SLA class Ⅰ molecules binding to swine-origin viral cytotoxic T lymphocyte epitope peptides in vitro[J].Frontiers in Microbiology,2017,8:2524.
[68] YU S,OPRIESSNIG T,KITIKOON P,et al.Porcine circovirus type 2(PCV2) distribution and replication in tissues and immune cells in early infected pigs[J].Veterinary Immunology and Immunopathology,2007,115(3-4):261-272.
[69] TRIBLE B R,ROWLAND R R R.Genetic variation of Porcine circovirus type 2(PCV2) and its relevance to vaccination,pathogenesis and diagnosis[J].Virus Research,2012,164(1-2):68-77.
[70] KARUPPANNAN A K,OPRIESSNIG T.Porcine circovirus type 2(PCV2) vaccines in the context of current molecular epidemiology[J].Viruses-Basel,2017,9(5):99.
[71] SIDLER X,SYDLER T,MATEOS J M,et al.Porcine circovirus type 2 pathogenicity alters host's central tolerance for propagation[J].Pathogens,2020,9(10):839.
[72] 董茂丽.猪圆环病毒2型小鼠T细胞免疫应答与表位疫苗初步鉴定[D].扬州:扬州大学,2021. DONG M L.Preliminary study of T cell epitopes-based vaccine against Porcine circovirus type 2 in mice[D].Yangzhou:Yangzhou University,2021.(in Chinese)
[73] 周文锋,张英婕,白一涵,等.猪圆环病毒2型全基因组序列分析及Cap蛋白CTL表位预测[J].中国畜牧兽医,2023,50(2):656-665. ZHOU W F,ZHANG Y J,BAI Y H,et al.Complete genome sequence analysis and Cap CTL epitopes prediction of Porcine circovirus type 2[J].China Animal Husbandry&Veterinary Medicine,2023,50(2):656-665.(in Chinese)
[74] 李海玲,张世伟,冯亚莉,等.欧亚类禽H1N1猪流感病毒研究进展[J].沈阳农业大学学报,2022,53(4):492-503. LI H L,ZHANG S W,FENG Y L,et al.Research progress in the study of Eurasian avian-like H1N1 Swine influenza viruse[J].Journal of Shenyang Agricultural University,2022,53(4):492-503.(in Chinese)
[75] ZHU R,XU S,SUN W,et al.Correction:HA gene amino acid mutations contribute to antigenic variation and immune escape of H9N2 Influenza virus[J].Veterinary Research,2022,53(1):112.
[76] CUI P,SHI J,WANG C,et al.Global dissemination of H5N1 Influenza viruses bearing the clade 2.3.4.4b HA gene and biologic analysis of the ones detected in China[J]. Emerging Microbes&Infections,2022,11(1):1693-1704.
[77] 陈艳,王飞飞,杨焕良,等.H3N2亚型猪流感病毒血凝素单克隆抗体的制备及其抗原表位的鉴定[J].中国预防兽医学报,2021,43(6):633-638. CHEN Y,WANG F F,YANG H L,et al.Preparation and epitope identification of monoclonal antibody against hemagglutinin protein of H3N2 Swine influenza virus[J].Chinese Journal of Preventive Veterinary Medicine,2021,43(6):633-638.(in Chinese)
[78] 乔传玲,陈艳,杨焕良,等.一种杂交瘤细胞株、类禽型H1N1猪流感病毒HA蛋白MAb、抗原表位及应用:202011244388.1[P].2021-07-20. QIAO C L,CHEN Y,YANG H L,et al.A hybridoma cell line,avian H1N1-like Swine influenza virus HA protein MAb,antigenic epitopes and applications:2202011244388.1[P].2021-07-20.(in Chinese)
[79] 张璞,陈建凯,赖月辉,等.猪瘟病毒检测和猪瘟疫苗研究进展[J].广东农业科学,2022,49(4):106-115. ZHANG P,CHEN J K,LAI Y H,et al.Research progress in Classical swine fever virus detection and classical swine fever vaccines[J].Guangdong Agricultural Sciences,2022,49(4):106-115.(in Chinese)
[80] ZHANG Y,NA D,ZHANG W,et al.Development of stable HEK293T cell pools expressing CSFV E2 protein:A potential antigen expression platform[J].Vaccine,2023,41(9):1573-1583.
[81] DESSELBERGER U.Rotaviruses[J].Virus Research,2014,190:75-96.
[82] 黄正阳,陈宇婧,黄克,等.猪A组轮状病毒HN2019-01株的分离鉴定及VP6基因序列分析[J].河南农业科学,2021,50(6):125-133. HUANG Z Y,CHEN Y J,HUANG K,et al.Isolation and VP6 gene sequence analysis of porcine group A Rotavirus HN2019-01 strain[J].Journal of Henan Agricultural Sciences,2021,50(6):125-133.(in Chinese)
[83] ZHANG N,QI J,FENG S,et al.Crystal structure of swine major histocompatibility complex class Ⅰ SLA-10401 and identification of 2009 pandemic swine-origin Influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides[J].Journal of Virology,2011,85(22):11709-11724.
[84] FAN S,WU Y,WANG S,et al.Structural and biochemical analyses of swine major histocompatibility complex class Ⅰ complexes and prediction of the epitope map of important Influenza A virus strains[J].Journal of Virology,2016,90(15):6625-6641.
[85] NING S,WANG Z B,QI P,et al.Crystallization of SLA-2*04:02:02 complexed with a CTL epitope derived from FMDV[J].Research in Veterinary Science,2020,128:90-98.
[86] TUN G S,ORECCHIA M A,SWALLOW K,et al.Selection of phage display designer whole gene fragment library and random peptide library to identify linear and conformational epitopes on a biosimilar infliximab molecule[J].Gastroenterology,2022,162(7):S1091.
[87] HAZEBROUCK S,PATIL S U,GUILLON B,et al.Immunodominant conformational and linear IgE epitopes lie in a single segment of Ara h 2[J].Journal of Allergy and Clinical Immunology,2022,150(1):131-139.
[88] MOISE L,GUTIERREZ A H,KHAN S,et al.New immunoinformatics tools for swine:Designing epitope-driven vaccines,predicting vaccine efficacy,and making vaccines on demand[J].Frontiers in Immunology,2020,11:563362.
[89] SRIVASTAVA P,JAIN C K.Computer aided reverse vaccinology:A game-changer approach for vaccine development[J]. Combinatorial Chemistry&High Throughput Screening,2023,26(10):1813-1821.
[90] 何玉龙,李高建,舒金琪,等.一种基于反向疫苗学技术的猪肺炎支原体多表位基因工程亚单位疫苗的制备方法及其应用:202111664581.5[P].2023-06-13. HE Y L,LI G J,SU J Q,et al.Preparation of a multi-epitope genetically engineered subunit vaccine against Mycoplasma pneumoniae in pigs based on reverse vaccinology technology and its application:202111664581.5[P].2023-06-13.(in Chinese)

Advances in Cytotoxic T Lymphocyte Epitopes of Porcine Viruses

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