A型猪流感病毒实时荧光定量PCR检测方法的建立及应用

›› 2010, Vol. 37 ›› Issue (9): 75-77.

• 生物技术 • Previous Articles Next Articles

Establishment and Application of a Real-time Quantitative PCR Method for Rapid Detection of Swine Influenza Virus A-type

OU YANG-Zhen yu1,TANG Lian-fei1, ZHU Zhong-wu1, XIAO Jia-yong1,MENG Fang1, HUANG Ping1, WU Yu-bai2

(1.Inspection and Quarantine Technology Center, Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410004,China;2.Animal Quarantine Department, Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410004,China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
Contact: TANG Lian-fei

Abstract

Abstract: The specific primers and probe were designed according to the region of genes of swine influenza virus A-type that recommend by WHO. A real-time fluorescent quantitative PCR method was established for detection of swine influenza virus A-type. Series of dilutions of recombinant plasmids were prepared and used to generate standard curves. The results showed that the real-time fluorescent quantitatine PCR was capable of detecting 100 copies of swine influenza virus A-type genes per 25 μL reaction system. The method was very sensitive and reprducibilitive, had been used for clinical laboratory testing of nasal swab samples, and could be used for rapid quantitative detection of swine influenza virus A-type.

Key words: swine influenza virus A-type; real-time quantitative PCR; H1N1 subtype

CLC Number:

S852.65⁺9.5

OU YANG-Zhen yu;TANG Lian-fei;ZHU Zhong-wu;XIAO Jia-yong;MENG Fang;HUANG Ping;WU Yu-bai. Establishment and Application of a Real-time Quantitative PCR Method for Rapid Detection of Swine Influenza Virus A-type[J]. , 2010, 37(9): 75-77.

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Establishment and Application of a Real-time Quantitative PCR Method for Rapid Detection of Swine Influenza Virus A-type

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