›› 2010, Vol. 37 ›› Issue (9): 213-216.

• 疾病防治 • Previous Articles     Next Articles

Laboratory Diagnosis of Dead Causes of Two Dogs

LIU Hua1,2, LI Gang-shan1, ZHANG Fu-qiang1, FAN Quan-shui1, YUAN Shu-you1, DU Qiang1, 
YIN Ge-fen2, LI Zuo-sheng1, ZHENG Ying1, WANG Shuang-yin1, QIU Wei1   

  1. (1.The Military Medical Institute of Center for Disease Control and Prevention,Chinese PLA Chengdu Military Command, Kunming 650032,China;2.College Animal Science and Technology, Yunnan Agricultural University, Kunming 650201,China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: QIU Wei

Abstract: The objective of this pepar was to identify the dead cause of two Kunming dogs. Canine intestinal contents were collected for bacteriological identification and virus identification. Bacteriological identification included crossed culture purification, gram staining, microscopy, biochemical tests, serum test and sensitivity test. Virus identification was done by PCR.PCR products were cloned into pMD18-T vector and then transformed into receptors bacteria DH5α. Positive clones were picked by identification and sequencing of plasmid. At last, the homology with reference strains known sequence was compared and analyzed. A resistant strain of Escherichia coli was isolated and a canine parvovirus was detected.Homology analysis showed that viral partial sequence amplified by the primers P1/P2 was 98.58%, compared with Cpv-b. The other viral partial sequence amplified by the primers P3/P4 was 92.1%. The dead causes of two dogs were the infection of Escherichia coli and canine parvovirus, respectively.

Key words: dog; Escherichia coli; canine parvovirus; laboratory diagnosis

CLC Number: