北京五区犬猫肠道冠状病毒感染的监测与分析

doi:10.16431/j.cnki.1671-7236.2023.12.028

Abstract

Abstract: 【Objective】 This study was aimed to investigate the intestinal Coronavirus infection in dogs and cats in Beijing,and to explore the correlation between canine and feline Coronavirus infection and age,season,etc.,in order to provide reference for the prevention and treatment of canine and feline Coronavirus disease.【Method】 Specific primers were designed according to the conserved regions of each pathogen.PCR or RT-PCR were used to detect canine and feline Coronavirus from 402 canine and feline anal swabs (192 canine and 210 feline samples),and to detect the mixed infection of canine and feline Parvovirus and Canine astrovirus (CaAstV).Chi-square test was used to analyze the correlation between Canine coronavirus (CCoV) and Feline coronavirus (FCoV) infection and season.Regression statistics were used to analyze the relationship between CCoV and FCoV infection and age.According to the clinical sample information,the immunization situation of dogs and cats in the five districts of Beijing was analyzed.【Result】 In 192 canine samples,the positive rate of CCoV was 44.27% (85/192),and the mixed infection rate of CCoV and Canine parvorius (CPV) was 34.12% (29/85), no CaAstV positive samples were detected.In 192 feline samples,the positive rate of FCoV was 45.71% (96/210),and the mixed infection rate of FCoV and Feline panleukopenia virus (FPV) was 60.42% (58/96).Among 192 canine samples,the positive rate of CCoV was 19.39% (19/98) in healthy dogs and 70.21% (66/94) in diseased dogs.Among 210 feline samples,the positive rate of FCoV was 24.41% (31/127) in healthy cats and 78.31% (65/83) in sick cats.The highest detection rate of CCoV in winter was 42.35% (36/85).The detection rate of FCoV in four seasons was similar,and the detection rate in winter was relatively high (29.17% (28/85)).In different age groups,the positive rate of CCoV was 28.6%-87.8%,and the positive rate of CCoV was 87.8% in dogs aged 0 to 3 months,which was the main group of CCoV incidence.The positive rate of FCoV was 36.4% to 53.8% in different age groups,and there was no significant difference among different age groups (P>0.05).【Conclusion】 The infection rate of intestinal coronavirus in dogs and cats in Beijing was relatively higher,and it was more prevalent in winter.There was no significant difference in the detection rates of CCoV and FCoV among dogs and cats of different age groups.The coinfection of Coronavirus and Parvovirus was more common in clinical practice.

Key words: dogs and cats; Coronavirus; Parvovirus; Astrovirus; epidemiology

CLC Number:

S852.74^＋6

CHEN Xin, ZHANG Yichen, LIU Ming, LI Shaohan, QIN Tong. Surveillance and Analysis of Intestinal Coronavirus Infection in Dogs and Cats in Five Districts of Beijing[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5067-5074.

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Surveillance and Analysis of Intestinal Coronavirus Infection in Dogs and Cats in Five Districts of Beijing

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