2013—2022年云南地区猪肠病毒G型的分子流行病学调查

doi:10.16431/j.cnki.1671-7236.2023.12.022

Abstract

Abstract: 【Objective】 This study was aimed to understand the prevalence and molecular characteristics of Enterovirus G (EV-G) in pig farms in Yunnan,so as to provide the theoretical basis for the prevention and control of EV-G in Yunnan.【Method】 RT-PCR basing on the primers from the highly conserved region of the 5'-untranslated region (UTR) of EV-G was used for the detection of EV-G-positive samples from clinical diarrhea samples of pigs collected in Yunnan from 2013 to 2022.The farm distribution,swine age composition and the type of positive samples were analyzed to determine the infection characteristics of EV-G in Yunnan.The complete sequence of EV-G VP1 gene was amplified using nested PCR specific primers.MegAlign software was used to analyze the similarity and key amino acid mutations of VP1 between Yunnan strains and the reference strains of 20 EV-G genotypes.A genetic evolutionary tree of VP1 gene was constructed by Mega 7.0 software.Additionally,the antigenic index of VP1 protein was analyzed using the Jameson-Wolf method in Protean software.【Result】 A total positive rate of 19.42% (377/1 941) for EV-G was observed in the clinical samples collected in Yunnan from 2013-2022.Among them,the EV-G infection rates in pig populations from Dali (54.54%),Yuxi (45.45%) and Honghe (43.24%) were significantly higher than that in other regions of Yunnan.The positive infection rates in suckling piglets (25.19%) and nursery pigs (24.68%) were higher than that in other pig age groups.The detection rate of positive cloacal swab (53.58%) was higher than that of intestinal contents samples (29.71%) and fecal samples (16.71%).According to the analysis of the similarity and genetic evolution of VP1 gene of EV-G showed that 17 strains from 20 Yunnan representatives belonged to EV-G1 genotype,2 strains belonged to EV-G6 genotype,and 1 strain belonged to EV-G17 genotype.VP1 protein of 17 Yunnan isolates of EV-G1 genotype exhibited significantly enhanced antigenic activity at positions 125-150 and 170-190,where multiple hydrophilic amino acid mutations were observed (P¹²⁷S,P¹⁴³S,G¹⁴⁶R and P¹⁸⁸S).【Conclusion】 Multiple genotypes such as EV-G1,EV-G6 and EV-G17 were prevalent in pig populations in Yunnan,with the EV-G1 type being the main genotype.VP1 protein of EV-G1 genotype Yunnan isolate exhibited significant antigenic activity at 125-150 and 170-190 amino acids,which might mediate the infection and epidemic of EV-G1 genotype strains in Yunnan.

Key words: Enterovirus G (EV-G); VP1; molecular epidemiology; genetic characteristics

CLC Number:

S852.65⁺1

WANG Wei, ZHU Pei, LI Zhanhong, XUE Xiaoyan, YANG Qinhong, LI Xue, LI Suhua, YAO Jun. Molecular Epidemiological Survey of Porcine Enterovirus Type G in Yunnan from 2013 to 2022[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5000-5012.

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Molecular Epidemiological Survey of Porcine Enterovirus Type G in Yunnan from 2013 to 2022

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