新型鸭呼肠孤病毒QY株σB蛋白基因特征、二级结构预测及其细胞抗原表位分析

doi:10.16431/j.cnki.1671-7236.2019.10.005

Abstract

Abstract: The purpose of this study was to analyze the genetic variation,structure and function of σB protein of a new duck reovirus (NDRV) QY strain.The coding sequences of σB protein of QY strain and 25 reference strains were obtained from GenBank database.Sequence alignment analysis and phylogenetic tree construction were carried out by Mega 6.0 software.Selective pressure analysis was carried out by Datamonkey software.Secondary structure and function of σB protein of QY strain and antigen epitopes of B and T cells were predicted by bioinformatics software.Similarity analysis showed that the amino acid similarity of NDRV QY strain with duck reovirus (DRV) isolated from other regions in China reached 94.9% to 98.9%,and the similarity with avian reovirus (ARV) and Muscovy duck reovirus (MDRV) were only 66.5% to 68.4% and 67.6% to 68.4%,respectively.Selective pressure analysis showed that σB protein was subjected to purification selective pressure,but one positively selected site was detected.The σB protein belonged to hydrophilic protein,had no signal peptide and transmembrane region,and contained potential O-glycosylation sites.Structural prediction analysis showed that σB protein had abundant secondary structures such as alpha helix,beta fold,beta turn and random coil.Epitope analysis showed that σB protein contained potential B- and T-cell epitopes.This study successfully predicted the gene characteristics,structure and function of σB protein of QY strain and analyzed its cell epitope,which laid a foundation for further understanding the immunological characteristics of the protein and developing a new NDRV vaccine.

Key words: new-type duck reovirus (NDRV); σB protein; genetic characteristics; secondary structure; epitope

CLC Number:

S852.65

WU Yangkai, FAN Wensheng, ZHANG Xuelian, LI Zhili, GUO Jinyue, LI Wenfeng, HUANG Shujian. Analysis for Characteristics of the New-type Duck Reovirus σB Protein and Prediction of Secondary Structure and B- and T-cell Epitopes[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2851-2859.

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Analysis for Characteristics of the New-type Duck Reovirus σB Protein and Prediction of Secondary Structure and B- and T-cell Epitopes

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