›› 2018, Vol. 45 ›› Issue (7): 1942-1948.doi: 10.16431/j.cnki.1671-7236.2018.07.027

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Molecular Characteristics Analysis and Antigen Epitope Prediction of F1L Gene of Orf Virus Isolate from Sichuan

SUN Zhengnan, MAO Congjian, ZHANG Huanrong   

  1. College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China
  • Received:2018-01-08 Online:2018-07-20 Published:2018-07-20


To determine the molecular characteristics of F1L gene of orf virus (ORFV) isolate from Sichuan,and predict the antigenic epitopes,this experiment used bioinformatics software to analyze ORFV F1L gene of Sichuan isolate and predict the secondary and tertiary structure and B cell epitopes of the encoded protein.The F1L gene was cloned,transformed and sequenced.The mutation of ORFV F1L gene from other regions was analyzed by Mega 7.0 software.The secondary structure of F1L protein was predicted by SOPMA server,and the hydrophilicity was predicted by DNAStar software.Surface accessibility,flexibility,and antigenicity were validated using ABCpred protocol, and the B-cell epitopes were exported.After using SWISS-MODEL server to predict the tertiary structure,re-validated the epitopes in the modeling area,final returned to the protein sequence alignment.The results showed that the ORFV F1L gene of Sichuan isolate was 1 029 bp in size and encoded 342 amino acids.F1L gene and its encoded protein had a certain variation compared with other regions.F1L protein predicted 9 potential B cell epitopes.Its 122 to 137 peptide was the optimal epitope after comparison of tertiary structure;The predicted ORFV F1L protein antigen of Sichuan isolates had certain specificity compared with other regions,the 124,131 and 137 amino acids were different in many strains of other regions.The results of this study provided theoretical basis for the research of ORFV genetic engineering vaccines and specific detection methods in Sichuan.

Key words: orf virus (ORFV); F1L gene; protein structure; B cell antigen epitopes

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