猫泛白细胞减少症病毒北京株NS1基因克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.12.003

Abstract

Abstract:

In order to study the molecular characteristics and biological function of NS1 gene of feline parvovirus (FPV),the NS1 gene of FPV-BJ 05 strain was amplified,cloned and sequenced.Nucleotide and amino acid homology analysis,signal peptide,transmembrane domain,B-cell preponderant epitope,phosphorylation site subcellular localization and protein structure and fuction,advanced structure of NS1 gene were predicted using bioinformatic softwares.The results showed that the length of NS1 gene was 2 007 bp,encoding 668 amino acids.The NS1 gene of FPV-BJ 05 shared 98.8% to 99.3% nucleotide homology with that of the other FPV strains,and the amino acid homology was 97.9% to 98.8%.Phylogenetic tree analysis showed that the NS1 protein of FPV-BJ 05 strain was classified as the same major branch with 3 FPV reference strains registered in GenBank,but it was a small independent branch due to amino acid mutation.The NS1 protein had neither a signal peptide nor a transmembrane domain,which was a non-secretory hydrophobic protein.The prediction results of B cell antigen parameters showed that NS1 protein had good flexibility,high antigenicity and surface accessibility,and it was predicted that the protein contained 13 dominant antigenic sites.62 phosphorylation sites,27 O-glycosylation sites and 2 N-glycosylation sites were found in NS1 protein.The result of subcellular localization showed that the probability of NS1 protein in the cytoplasm and nucleus was higher,which were 69.6% and 17.4% respectively.The prediction of secondary structure revealed that α-helix (39.37%),extended chain (15.42%),random coil (39.37%) and β-turn (5.84%),and the tertiary structure of NS1 protein was curved spiral structure.This study provided theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of FPV in Beijing.

Key words: feline panleukopenia virus (FPV); NS1 gene; bioinformatics; protein structure prediction

CLC Number:

S852.65⁺9.2

LIU Qi, SHI Lijun, YUAN Weifeng, LIANG Ruiying, LI Jinxiang, CUI Shangjin. Cloning and Bioinformatic Analysis of NS1 Gene of Feline Parvovirus in Beijing[J]. , 2018, 45(12): 3327-3336.

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Cloning and Bioinformatic Analysis of NS1 Gene of Feline Parvovirus in Beijing

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