›› 2018, Vol. 45 ›› Issue (12): 3305-3316.doi: 10.16431/j.cnki.1671-7236.2018.12.001

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Prediction and Analysis of Structure and Function of Porcine Connexin 43 (GJA1) Protein

DAI Kaiyu1, CAO Yue1, GAO Zhongcheng1, WANG Haifei1, BAO Wenbin1,2, WU Shenglong1,2   

  1. 1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Seed-breeding and Innovation Team of Jiangsu Province Modern Agriculture(Swine) Industry Technical System, Yangzhou 225009, China
  • Received:2018-03-28 Online:2018-12-20 Published:2018-12-21

Abstract:

To explore the structure and function of porcine connexin 43 (GJA1) protein,this study compared the coding sequences of GJA1 protein in 16 different species to analyze evolution and phylogeny relationship.The amino acid sequence information of porcine GJA1 protein such as physical and chemical properties,protein modification sites,transmembrane regions,subcellular localization,secondary and tertiary structures,polymorphic sites,functional regions and interaction proteins were analyzed by the related bioinformatic softwares and databases.Phylogenetic analysis results showed the variation of GJA1 protein did not reach saturation,the protein sequence displayed small genetic distance and high homology among 16 species.The porcine GJA1 protein had the greatest genetic relationship with that of equine,and the classification in the evolutionary tree also confirmed it.The protein structure analysis results indicated that the porcine GJA1 protein was composed of 20 kinds of amino acids with the molecular weight of 43.08 ku and theoretical isoelectric point of 8.88.It was predicted that the instability index of porcine GJA1 protein was 44.06,the lipid solubility index was 82.67,and its hydrophobic average coefficient was -0.240.There were 4 transmembrane regions,6 O-GlcNAc sites,38 phosphorylation sites and no signal peptide.The GJA1 protein was most likely exist in the plasma membrane and the Golgi apparatus,and its secondary and tertiary structures were mainly comprised of α-helix and random coil.The protein function analysis results demonstrated that the porcine GJA1 protein had 2 superfamily functional regions and 6 missense mutation sites in its CDS sequence.In addition,the porcine GJA1 protein interacted with ZO-1,Cx36 and many protein kinases.This study systematically revealed the structure and function of porcine GJA1 protein by bioinformatics analysis,which could provide a theoretical reference for further researches on the genetic regulatory mechanism of GJA1 protein in pigs.

Key words: porcine; connexin 43(GJA1); protein structure; protein function

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