牛乳头状瘤病毒贵州株L1基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2016.11.005

Abstract

Abstract:

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.

Key words: bovine papillomavirus（BPV); L1 gene; bioinformatics analysis; protein structure prediction

CLC Number:

Q785

ZHANG Hai, ZHOU Bi-jun, YANG Zhong-cheng, LIAO Mei, WANG Kai-gong, WEN Ming, CHENG Zhen-tao, WANG Wei, FENG Xu-fang. Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province[J]. , 2016, 43(11): 2834-2843.

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Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

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