›› 2018, Vol. 45 ›› Issue (9): 2368-2376.doi: 10.16431/j.cnki.1671-7236.2018.09.003

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Comparative Analysis of VIL-10 Gene Between the Vaccine and Wild Strains of Orf Virus

FENG Qian, WU Jinyan, CHEN Yan, LIU Yongjie, ZHANG Keshan, SHANG Youjun, LIU Xiangtao   

  1. Key Laboratory of Animal Virology of the Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China
  • Received:2018-03-01 Online:2018-09-20 Published:2018-09-26


To analyze the different characteristics between VIL-10 gene from the vaccine and wild strains of orf virus (ORFV),a pair of specific primers was designed according to ORFV NZ2 strain VIL-10 gene sequence reported in GenBank.The genomic DNA was taken as templates extracted from the vaccine and wild strains of ORFV.The full lengths of VIL-10 gene were obtained by PCR and then sequenced.The variation of nucleic acids,amino acids and protein structures were predicted and analyzed using bioinformatics software.The results showed that VIL-10 gene from ORFV vaccine strain and wild strain shared 94.4% identity at nucleotide level and the main difference was a single base mutation,deletion in the nucleotide sequence which occurred at 132 to 134 bp.92.5% identity at amino acid level and a mutation of 15 amino acid sites occurred,of which the asparagine was missing at 42 amino acid position.There were some differences in primary structure and physical and chemical properties of protein,secondary structure,tertiary structure,physicochemical properties,epitope parameters and the presence or absence of signal peptide.However,no transmembrane domains were identified both in the vaccine and virulent strains of ORFV.Phylogenetic analysis revealed that the wild strain and the vaccine strain belonged to different branches and the genetic relationship was far away.The results suggested that the VIL-10 gene of wild virus strains and vaccine strains had obvious mutations,and these mutations might be related to the virulence of the vaccine strains.

Key words: orf virus (ORFV); vaccine strain; wild strain; VIL-10 gene; comparative analysis

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