重庆地区羊口疮病毒的分离鉴定及F1L基因的原核表达

doi:10.16431/j.cnki.1671-7236.2015.06.011

Abstract

Abstract: The assay was aimed to isolate,culture and identify Orf virus (ORFV) in Chongqing area,and investigate the expression of the F1L gene sequence in E.coli,the virus in the scab samples collected from suspected ORFV infected lamb was isolated in MDBK cell.Moreover,the F1L gene was amplified by PCR and ligated into pET-32a for expression in E.coli.The recombinant plasmid pET-32a-F1L was constructed with the introduction of restriction enzymes NotⅠand EcoRⅠ.The isolated virus was ORFV.SDS-PAGE analysis and Western blotting showed that the target protein was highly expressed with 57.4 ku in lenght at 5 h in E.coli as inclusion bodies and had good reactivity.

Key words: Orf virus (ORFV); isolation and identification; F1L gene; prokaryotic expression

CLC Number:

YANG Yu, FENG Jie, LIU Ai, ZHANG Su-hui, XU Guo-yang, FENG Jiang, LIU Zong-sheng, XIAN Si-mei. Isolation and Identification of Orf Virus in Chongqing Area, and Prokaryotic Expression of F1L Gene[J]. , 2015, 42(6): 1396-1401.

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Isolation and Identification of Orf Virus in Chongqing Area, and Prokaryotic Expression of F1L Gene

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