PRRSV美洲型经典株、变异株和疫苗株多重RT-PCR鉴别检测方法的建立及应用

doi:10.16431/j.cnki.1671-7236.2017.03.036

Abstract

Abstract:

To establish a rapid method for differential detection of classical, highly pathogenic and TJM-F92 vaccine strains of North American genotype porcine reproductive and respiratory syndrome virus (PRRSV), a multiple RT-PCR assay was established. In this assay, two pairs of primers were designed according to the genomic sequences of classical, highly pathogenic and TJM-F92 vaccine strains of PRRSV. The assay could only detect PRRSV, but not detect CSFV, FMDV, PRV and PCV2. The detection limit of the method was as little as 1.13×10³ copies/μL of templates. The established assay was successfully used to detect 349 clinical samples and 119 samples were positive for PRRSV, of which 5 samples were positive for classical PRRSV (C-PRRSV), 107 samples for highly pathogenic PRRSV (HP-PRRSV) and 7 samples for TJM-F92 vaccine strain (V-PRRSV), while 7 samples were positive for HP-PRRSV and V-PRRSV. The results indicated that the established multiple RT-PCR assay could be used for differential detection and epidemiological investigation of PRRSV.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); North American genotype; multiplex RT-PCR; wild-type strain; vaccine strain

CLC Number:

S862.65⁺1

SHI Kai-chuang, XU Xin-ting, HU Jie, SU Yan-qiong, LU Wen-jun, CHEN Han-zhong. Establishment and Application of a Multiplex RT-PCR Assay for Differential Detection of Classical, Highly Pathogenic and Vaccine Strains of North American Genotype PRRSV[J]. , 2017, 44(3): 879-887.

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Establishment and Application of a Multiplex RT-PCR Assay for Differential Detection of Classical, Highly Pathogenic and Vaccine Strains of North American Genotype PRRSV

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