牦牛泌乳期垂体组织比较转录组学分析

doi:10.16431/j.cnki.1671-7236.2019.03.022

Abstract

Abstract:

This study was aimed to explore the transcriptome difference of pituitary gland in yak with different milk production during lactation,and provide a reference for further elucidating the mechanism of lactation pituitary gland in yak.The total RNA was extracted from pituitary gland tissue,high-throughput sequencing and analysis of pituitary tissue transcriptome during high lactation calf and low-yield calf lactation were performed using RNA-Seq technology.By the comparison and analysis of the transcriptome data for the pituitary gland tissue of high-yielding yak and low-yielding yak,114 differential genes were screened out,of which 55 were up-regulated and 59 were down-regulated.Further functional analysis indicated that these differential expressed genes involved in multiple classes based on GO analysis and KEGG pathways.The GO analysis result showed that the functions of the differential genes were closely related to the amino acids metabolism and other biological processes.KEGG pathway analysis result revealed that these genes were mostly enriched in the pathway of cell adhesion molecule pathway.Moreover,several other pathways related to immune such as Staphylococcus aureus infection and leishmaniasis also showed significant enrichment.The pituitary gland transcriptome of high-yielding yak and low-yielding yak during lactation had been compared,and the related differentially expressed genes had been screened and analyzed,which provided new ideas for improving the milk production of yaks.

Key words: yak; pituitary gland; transcriptome; comparative analysis; molecule mechanism

CLC Number:

S814.8

ZHU Yuxing, LIU Jun, AI Yi, CHEN Tong, DENG Zhihe, HE Shiming, WU Jinbo, LAN Daoliang. Comparative Transcriptome Analysis of Lactation Pituitary Gland in Yak[J]. , 2019, 46(3): 824-831.

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Comparative Transcriptome Analysis of Lactation Pituitary Gland in Yak

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