China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (4): 1216-1224.doi: 10.16431/j.cnki.1671-7236.2020.04.028

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Epidemiological Investigation and VP1 Gene Sequence Analysis of Porcine Enterovirus Type G in Guangxi

YANG Chunjie, LIU Xueting, MI Xue, CHEN Ying, WEI Zuzhang, HUANG Weijian, OUYANG Kang   

  1. College of Veterinary Science and Techology, Guangxi University, Nanning 530005, China
  • Received:2019-10-22 Online:2020-04-20 Published:2020-04-17

Abstract: To analyze the prevalence and molecular genetic evolution of porcime enterovirus G (EV-G) in Guangxi,222 clinical diarrhea samples were collected in Guangxi from 2017 to 2018 in this study,EV-G detection and epidemiological investigation were conducted,as well as VP1 gene amplification,cloning and sequence analysis.According to the results of epidemiological survey,the positive rate of EV-G samples in Guangxi from 2017 to 2018 was 6.76% (15/222),while the positive rate of pig farms was as high as 16.98% (9/53).It was noteworthy that the positive rate of EV-G samples in Guangxi had increased from 4.55% in 2017 to 10.00% in 2018,which showed a significant increase.At the same time,the detection rate of this virus was higher in winter and spring.Sequence homology analysis showed that the nucleotide homology between the two EV-G VP1 genes obtained in this study was 99.2% and the amino acid homology was 98.8%.Compared with domestic and foreign strains,the nucleotide and amino acid identity was 62.4% to 80.1% and 45.7% to 71.7%,respectively.Genetic evolution analysis showed that the two EV-G VP1 sequences obtained in this study both belonged to the G1 subtype in the same branch,and were closely related to the American isolate 13-03212. The results of amino acid sequence comparison showed that the two strains of EV-G VP1 had great variation at multiple sites.Antigen index analysis showed that the antigenicity of EV-G in Guangxi had a large variation.The results of this study indicated that there was a certain degree of EV-G infection in Guangxi during 2017 to 2018.This study provided a reference for the EV-G epidemic profile and the molecular characteristics of EV-G.

Key words: porcine enterovirus G (EV-G); epidemiology; sequence analysis; antigenicity

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